Strategies for gene disruption and expression in filamentous fungi

Mei, Yan‐Zhen; Zhu, Yali; Huang, Pengwei; Yang, Qian; Dai, Chuan‐Chao

doi:10.1007/s00253-019-09953-2

Cited by 34 publications

(17 citation statements)

References 161 publications

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“…Different strategies reemployed in order to investigate functional genomic as shown in Table 4 and Fig. 3, including the use of selection markers, conducting transformations, and genetic manipulations (Mei et al 2019;He et al 2019).…”

Section: Strategies For a Oryzae Functional Genomicsmentioning

confidence: 99%

The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool

Daba

Mostafa

Elkhateeb

2021

Bioresour. Bioprocess.

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Aspergillus oryzae (A. oryzae) is a filamentous micro-fungus that is used from centuries in fermentation of different foods in many countries all over the world. This valuable fungus is also a rich source of many bioactive secondary metabolites. Moreover, A. oryzae has a prestigious secretory system that allows it to secrete high concentrations of proteins into its culturing medium, which support its use as biotechnological tool in veterinary, food, pharmaceutical, and industrial fields. This review aims to highlight the significance of this valuable fungus in food industry, showing its generosity in production of nutritional and bioactive metabolites that enrich food fermented by it. Also, using A. oryzae as a biotechnological tool in the field of enzymes production was described. Furthermore, domestication, functional genomics, and contributions of A. oryzae in functional production of human pharmaceutical proteins were presented. Finally, future prospects in order to get more benefits from A. oryzae were discussed.

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Section: Strategies For a Oryzae Functional Genomicsmentioning

confidence: 99%

The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool

Daba

Mostafa

Elkhateeb

2021

Bioresour. Bioprocess.

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“…The Cas9–sgRNA CRISPR array, with a marker (RNP mediated) or marker‐free, needs a host‐specific vector for delivery to the fungal cell [124]. Both CRISPR‐editing strategies have been used in P. chrysogenum .…”

Section: Major Challenges In Designing Crispr/cas9 For Filamentous Fungimentioning

confidence: 99%

CRISPR/Cas9‐based genome engineering: A new breakthrough in the genetic manipulation of filamentous fungi

Ullah

Lin

Xie

et al. 2020

Biotech and App Biochem

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Filamentous fungi have several industrial, environmental, and medical applications. However, they are rarely utilized owing to the limited availability of full‐genome sequences and genetic manipulation tools. Since the recent discovery of the full‐genome sequences for certain industrially important filamentous fungi, CRISPR/Cas9 technology has drawn attention for the efficient development of engineered strains of filamentous fungi. CRISPR/Cas9 genome editing has been successfully applied to diverse filamentous fungi. In this review, we briefly discuss the use of common genetic transformation techniques as well as CRISPR/Cas9‐based systems in filamentous fungi. Furthermore, we describe potential limitations and challenges in the practical application of genome engineering of filamentous fungi. Finally, we provide suggestions and highlight future research prospects in the area.

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“…There is significant growth in the number of catalogued fungal strains, with an estimated 95% of fungal species estimated to be undescribed yet [2], and the scientific community is building an increasingly vast body of knowledge around these complex and valuable organisms. The large and growing interest in filamentous fungi mostly stems from the fact that their metabolic processes can be used to produce and refine a wide range of products and solutions [3] that create value for science, industries, and consumers.…”

Section: Filamentous Fungimentioning

confidence: 99%

Recent advances in the intellectual property landscape of filamentous fungi

Hüttner

Johansson²,

Teixeira³

et al. 2020

Fungal Biol Biotechnol

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For centuries, filamentous fungi have been used in the making of food and beverages, and for decades for the production of enzymes and pharmaceuticals. In the last decades, the intellectual property (IP) landscape for fungal technology has seen an ever increasing upward trend, introducing new and promising applications utilising fungi. In this review, we highlight fungi-related patent applications published during the last 5 years (2015–2020), identify the key players in each field, and analyse future trends. New developments in the field of fungal technology include the increased use of filamentous fungi as a food source (mycoprotein), using fungi as biodegradable materials, in wastewater treatment, in integrated biorefineries and as biological pest agents. Biotechnology companies in Europe and the US are currently leading when it comes to the number of patents in these areas, but Asian companies and research institutes, in particular in China, are becoming increasingly important players, for example in pesticide formulation and agricultural practices.

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Strategies for gene disruption and expression in filamentous fungi

Cited by 34 publications

References 161 publications

The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool

The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool

CRISPR/Cas9‐based genome engineering: A new breakthrough in the genetic manipulation of filamentous fungi

Recent advances in the intellectual property landscape of filamentous fungi

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