Strategies for increasing lipid accumulation and recovery fromY. lipolytica: A review

Abstract

Microbial-based biodiesel is produced by transesterification of lipids extracted from microbial cells, and is considered as a potential replacement of fossil fuel due to its advantages in reducing greenhouse gas emissions. Yarrowia lipolytica is one of the most studied oleaginous yeasts able to produce lipids under some fermentation conditions and is considered as a potential industrial host for biodiesel production. Several approaches have been evaluated to increase the economical attraction of biodiesel production from Y. lipolytica lipids. In this review, we highlighted the different strategies reported in the literature, allowing this yeast to achieve high lipid accumulation. These include metabolic engineering strategies, the use of low-cost effective substrates, and the optimization of the cultivation conditions for higher lipid productivity and less operating cost. We also summarized the most effective cell disruption technologies that improve the extraction efficiencies of lipids from Y. lipolytica.

Lipid Readjustment in Yarrowia lipolytica Odd-Chain Fatty Acids Producing Strains

“…Over the past several years, great progress has been made to improve lipid and lipid-derivatives production by Y. lipolytica [ 48 , 49 , 50 ]. Different strategies have been used for increasing lipid accumulation and recovery from Y. lipolytica [ 51 , 52 ].…”

“…Similar lipid content was previously reported by Sekova and coworkers [ 22 ] and in our previous reports [ 1 ]. Additional genetic modifications could be introduced to further improve the lipid content in odd-chain-producing strains, as those described in the recent review by El kantar and Koubaa [ 52 ].…”

Yarrowia lipolytica as an Alternative and Valuable Source of Nutritional and Bioactive Compounds for Humans

“…It is known that Y. lipolytica growing in oily substrates accumulates huge amounts of lipids intracellularly as storage lipid bodies (more than 40%), referred to as single-cell oils (SCOs) [ 1 , 119 , 120 ]. However, the production de novo and the accumulation ex novo of SCOs depends on the type of strains and the culture substrates, and the two processes can occur simultaneously [ 121 ].…”