The functioning and connectivity of the olfactory system, which produces our sense of smell, has recently been attracting increased attention from neuroscientists, particularly since the award of the 2004 Nobel Prize to Buck and Axel for their research in this field. To study the olfactory system further, it would be advantageous to develop methods for keeping isolated brain tissue viable for long periods of time. This study examines the morphology, electrophysiology and survival of cells in brain slices that have been kept in culture, in comparison to acute slices. We prepared parasagittal slices that contained the olfactory bulb (OB), the piriform cortex (PC) and the lateral olfactory tract (LOT). Such slices would be particularly useful in studies that depend upon intact connectivity between the OB and the PC. In this study, we identified differences in the synaptic and intrinsic properties between pyramidal neurons in the acute and cultured slices. Also, the GABAergic interneurons appear not to have been affected. Therefore, we concluded that these slices would be suitable for use in studying some aspects of the olfactory system but only once the differences identified have been investigated further.