Strategies for periodontal ligament cell viability: An overview

Abstract

The long-term success of the reimplanted teeth is related to the maintenance of periodontal ligament (PDL) cell viability. Dental tissues are unique in comparison to most other tissues in the body due to their marked capacity for regeneration. Understanding the circumstances leading to repair and regeneration in oral tissues has been a formidable challenge. Numerous storage media have been introduced by many authors that help to maintain the PDL cell viability. To present an overview of the various available storage media. A literature search for the past 20 years was performed across the Internet database (Pubmed) and relevant citations using the keywords PDL cell viability, tooth avulsion, storage media, and the combination of all to retrieve around (n=225) citations. Articles that included follow-up of intervention for avulsed and re-implanted teeth were considered (n=44) and some literature review from well-known text books were considered. Literature supports that moist storage appears to be a more productive approach to optimize PDL cell survival. However, no medium is ideal and in vivo studies are inadequate.

Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

“…In this sense, various storage media have been investigated for their ability to maintain the viability of PDL cells for as long as possible. These storage media include milk, culture media, Viaspan, Hank's Balanced Salt Solution (HBSS), Gatorade®, propolis 8,9 , soy milk 10,11 , and coconut water (CW) [11][12][13][14][15] .…”

Avulsed Tooth - A Storage Medium Dilemma an Update

“…Considering the unphysiologic media, tap water although is the most convenient medium, it causes rapid cell lysis similar to dry storage due to its hypotonicity, unphysiological pH and osmolality and microbial contamination [8,13,14] and therefore Ozan et al [15] in their study concluded that water was worse in comparison to saline [15]. Likewise saliva emphasizes similar properties as water and therefore inefficient to maintain the cellular integrity [16][17][18][19].…”

“…Likewise saliva emphasizes similar properties as water and therefore inefficient to maintain the cellular integrity [16][17][18][19]. Even though saline solution has a physiological pH and osmolality, it does not contain the essential ions and cell nutrients for cell survival [13].…”

Various Transport Media for Avulsed Tooth: A Review.

“…Nonetheless, in the absence of another storage medium, they may be used instead of water or saline for short periods of time. 22,26 Viaspan:…”