Strategies for Preparing Albumin-based Nanoparticles for Multifunctional Bioimaging and Drug Delivery

An, Feifei; Zhang, Shun

doi:10.7150/thno.19365

Cited by 378 publications

(239 citation statements)

References 257 publications

(270 reference statements)

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“…These results suggested that uncoated ZnO NMs could induce cytotoxicity and alterations of expression of ER stress‐apoptosis genes, which could be dependent on the size and shape of ZnO NPs. To reduce the toxicity associated with ZnO NM exposure, it may be necessary to use surface coating to change the interactions between ZnO NMs and cells (Cao et al, ; Liu et al, ), or use NMs based on biocompatible materials other than ZnO for biomedical purposes (An & Zhang, ; Wang et al, ). Alternatively, targeted drug delivery to realize accumulation of toxic ZnO NMs at specific sites could also be considered (Cai et al, ; Feng, Ding, Gref, & Shen, ; Shi et al, ; Wang et al, ).…”

Section: Discussionmentioning

confidence: 99%

The toxicity of ZnO nanomaterials to HepG2 cells: the influence of size and shape of particles

Yan

Jiang

Liu

et al. 2018

J of Applied Toxicology

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Understanding the possible role of physicochemical properties in determining the toxicity of ZnO nanomaterials (NMs) is crucial for the safe use of ZnO-based materials. In this study, we synthesized four types of ZnO NMs, and characterized them as ZnO nanorods (NRs; length 400-500 nm, diameter 150-200 nm), ZnO Mini-NRs (length 50-100 nm, diameter 15-20 nm), amorphous ZnO microspheres (a-ZnO MS) and crystalline ZnO MS (c-ZnO MS; the a/c-ZnO MS are nanoflowers with an extensive growth of sheet-like structures). ZnO NMs and ZnO Mini-NRs were significantly more cytotoxic than a/c-ZnO MS, and this trend was similar in both HepG2 cells and human umbilical vein endothelial cells. Intracellular reactive oxygen species was only modestly induced by c-ZnO MS, whereas intracellular Zn ions were dose-dependently increased in HepG2 cells by the exposure of all types of ZnO NMs. The expression of endoplasmic reticulum stress marker DDIT3 was induced following an order of ZnO NRs > a-ZnO MS > c-ZnO MS > ZnO Mini-NRs, and the apoptosis gene CASP12 was induced following an order of a-ZnO MS > ZnO NRs > c-ZnO MS > ZnO Mini-NRs. Combined, these results suggested that ZnO NM-induced cytotoxicity and expression of endoplasmic reticulum stress-apoptosis genes could be influenced by the size and shape of ZnO NMs.

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Section: Discussionmentioning

confidence: 99%

The toxicity of ZnO nanomaterials to HepG2 cells: the influence of size and shape of particles

Yan

Jiang

Liu

et al. 2018

J of Applied Toxicology

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“…We used our novel nanoparticles coated with elastin antibody that only targets degraded elastin while sparing healthy elastin to deliver PGG to the AAA site 3,6,13,26 . We chose albumin-based NPs as they are proven to be non-toxic 27 and are used to deliver paclitaxel in patients (Abraxane). We previously demonstrated that EL-PGG-NPs have no hepatic toxicity for systemic delivery and these particles are targeted at the aneurysmal site 3 .…”

Section: Discussionmentioning

confidence: 99%

Nanoparticle-based targeted delivery of pentagalloyl glucose reverses elastase-induced abdominal aortic aneurysm and restores aorta to the healthy state in mice

Dhital

Vyavahare

2019

Preprint

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Abbreviations AAA -Abdominal aortic aneurysm BSA -Bovine serum albumin DIR -1,1-Dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide EL-GNP -elastin antibody conjugated gold nanoparticle EL-NP -Elastin antibody-conjugated blank nanoparticles EL-PGG-NP -Elastin antibody-conjugated and PGG-loaded albumin NPs FACS -Fluorescence-activated cell sorting MMP-Matrix metalloproteinase NPs -Nanoparticles PGG-Pentagalloyl glucose TGF--Tumor Growth Factor beta IL-Interleukine ABSTRACT Aim: Abdominal aortic aneurysms (AAA) is a life-threatening weakening and expansion of the abdominal aorta due to inflammatory cell infiltration and gradual degeneration of extracellular matrix (ECM). There are no pharmacological therapies to treat AAA. We tested the hypothesis that nanoparticle (NP) therapy that targets degraded elastin and delivers anti-inflammatory, anti-oxidative, and ECM stabilizing agent, pentagalloyl glucose (PGG) will reverse advance stage aneurysm in an elastase-induced mouse model of AAA. Method and Results:Porcine pancreatic elastase (PPE) was applied periadventitially to the infrarenal aorta in mice and AAA was allowed to develop for 14 days. Nanoparticles loaded with PGG (EL-PGG-NPs) were then delivered via IV route at 14-day and 21-day (10 mg/kg of body weight). A control group of mice received no therapy. The targeting of NPs to the AAA site was confirmed with fluorescent dye marked NPs and gold NPs.Animals were sacrificed at 28-d. We found that targeted PGG therapy reversed the AAA by decreasing matrix metalloproteinases MMP-9 and MMP-2, and the infiltration of macrophages in the medial layer. The increase in diameter of the aorta was reversed to healthy controls. Moreover, PGG treatment restored degraded elastic lamina and increased the circumferential strain of aneurysmal aorta to the healthy levels. Conclusion:Our results support that site-specific delivery of PGG with targeted nanoparticles can be used to treat already developed AAA. Such therapy can reverse inflammatory markers and restore arterial homeostasis.

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“…Drug release from nanoparticles is a process attributed to diffusion following dissociation of the albumin molecules forming the nanoparticle. 31 The initial drug release can be considered a burst release of deposited or weakly bound drug molecules on the surface of nanoparticles. The cumulative release was greater in ANPs+ATP than in EM-ANPs+ATP, which likely could be due to the fact that the EMs coating contribute to the stability of the nanoparticle by affecting the dissociation of albumin molecules in the nanoparticles due to its lipidic component.…”

Section: In Vitro Drug Releasementioning

confidence: 99%

<p>ATP-loaded biomimetic nanoparticles as controlled release system for extracellular drugs in cancer applications</p>

Díaz-Saldívar

Huidobro‐Toro

2019

IJN

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Purpose The antitumoral effect of ATP requires its accumulation in the extracellular space to interact with membrane receptors in target cells. We propose the use of albumin nanoparticles (ANPs) coated with erythrocyte membranes (EMs) to load, deliver, release, and enhance the extracellular anticancer activity of ATP. Materials and methods ANPs were synthesized by desolvation method and optimal values of pH, albumin concentration, and ethanol volume were determined. EMs were derived from erythrocyte lysates and were coated on to ANPs using an extruder. Size was determined by transmission electron microscopy (TEM) and hydrodynamic size and zeta potential were determined by dynamic light scattering. Coating of the ANPs with the EMs was verified by TEM and confocal microscopy. Nanoparticle cell uptake was analyzed by confocal microscopy using HeLa and HEK-293 cell cultures treated with nanoparticles stained with 1,1′-diocta-decyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) for EM-ANPs and Alexa 488 for ANPs. Cell viability was analyzed by [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) and Annexin V/propidium iodide assays. Results Optimal values of ANP preparation were as follows: pH=9, 10 mg/mL albumin concentration, and 2.33±0.04 mL ethanol volume. Size distributions as analyzed by TEM were as follows: ANPs =91.9±4.3 nm and EM-ANPs =98.3±5.1 nm; hydrodynamic sizes: ANPs =180.5±6.8 nm and EM-ANPs =197.8±3.2 nm; and zeta potentials: ANPs =17.8±3.5 mV, ANPs+ATP =−13.60±0.48 and EM-ANPs =−13.7±2.9 mV. The EMs coating the ANPs were observed by TEM and confocal microscopy. A fewer number of internalized EM-ANPs+ATP compared to non-coated ANPs+ATP was observed in HeLa and HEK-293 cells. Cell viability decreased up to 48.6%±2.0% with a concentration of 400 µM ATP after 72 hours of treatment and cell death is caused mainly via apoptosis. Conclusion Our current results show that it is possible to obtain nanoparticles from highly biocompatible, biodegradable materials and that their coating with EMs allows the regulation of the internalization process in order to promote extracellular activity of ATP.

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Strategies for Preparing Albumin-based Nanoparticles for Multifunctional Bioimaging and Drug Delivery

Cited by 378 publications

References 257 publications

The toxicity of ZnO nanomaterials to HepG2 cells: the influence of size and shape of particles

The toxicity of ZnO nanomaterials to HepG2 cells: the influence of size and shape of particles

Nanoparticle-based targeted delivery of pentagalloyl glucose reverses elastase-induced abdominal aortic aneurysm and restores aorta to the healthy state in mice

<p>ATP-loaded biomimetic nanoparticles as controlled release system for extracellular drugs in cancer applications</p>

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