Strategies for protein-based nanofabrication: Ni2+-NTA as a chemical mask to control biologically imposed symmetry

Dabrowski, Michael J.; Chen, Jing Ping; Shi, Huaiqiu; Chin, Wei Chun; Atkins, William M.

doi:10.1016/s1074-5521(98)90662-7

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…In addition, bis-NTA tethers MBP-VLDLR-His 6 via its C-terminal His 6 tag, which orients the virus-binding moiety of the receptor away from the surface, rendering it highly accessible, while the coupling of protein to the NHS surface can occur for all exposed lysine residues leading to a more randomized orientation of the receptor molecules on the substrate. The advantage of oriented immobilization for protein function has been demonstrated in the context of other studies − . Here, we have shown a particular realization of this principle, which can be employed in the orthogonal biofunctionalization of micropatterned surfaces.…”

Section: Resultsmentioning

confidence: 65%

Receptor Arrays for the Selective and Efficient Capturing of Viral Particles

et al. 2009

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We describe microarrays of receptors on gold/glass substrates for the selective capturing of viral particles at high density. Microscale gold squares were surface-modified with alkanethiol derivatives which enabled the immobilization of the His(6)-tagged virus-binding domain from the very-low density lipoprotein (VLDL) receptor. The free glass areas surrounding the gold squares were passivated with a dense film of poly(ethylene glycol) (PEG). As assessed by atomic force microscopy, human rhinovirus particles were captured onto the VLDL-receptor patches with a high surface coverage but were effectively repelled by the PEG layer, resulting in a 330 000-fold higher density of the particles on the gold as compared to the glass surfaces. The metal chelate-based coupling strategy was found to be superior to two alternative routes, which used the covalent coupling of viral particles or viral receptors to the substrate surface. The high-density receptor arrays were employed for sensing and characterizing viral particles with so far unprecedented selectivity.

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Section: Resultsmentioning

confidence: 65%

Receptor Arrays for the Selective and Efficient Capturing of Viral Particles

et al. 2009

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confidence: 99%

“…The materials are suspended in solution or gas phase and are assembled at desired locations (receptors) on a substrate using specific interactions. Most actively investigated areas, currently, use protein recognition, 19,20 DNA hybridization, 9,21,22 hydrophobicity/hydrophilicity, surface tension and self-assembled monolayers, 10 topography-directed concepts, [23][24][25] magnetic 11 and dielectrophoretic assembly and transport, 22,[26][27][28] and electrostatic forces. [12][13][14][15][16][17][18]25,27 In recent years there has been an increased focus on the use of long range electrostatic forces to direct the assembly of nanomaterials since it can potentially be used to assemble a vast variety of materials (magnetic, nonmagnetic, insulating, semiconducting, conducting, organic, and inorganic materials) without altering the surface chemistry of the materials.…”

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confidence: 99%

Charging Process and Coulomb-Force-Directed Printing of Nanoparticles with Sub-100-nm Lateral Resolution

Barry

Jacobs

2005

Nano Lett.

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This article reports on a new charging process and Coulomb-force-directed assembly of nanoparticles onto charged surface areas with sub-100-nm resolution. The charging is accomplished using a flexible nanostructured thin silicon electrode. Electrical nanocontacts have been created as small as 50 nm by placing the nanostructured electrode onto an electret surface. The nanocontacts have been used to inject charge into 50 nm sized areas. Nanoparticles were assembled onto the charge patterns, and a lateral resolution of 60 nm has been observed for the first time. A comparison of the nanoparticle patterns with the surface potential distribution recorded by Kelvin probe force microscopy (KFM) revealed a mismatch in the lateral resolution. One possible explanation is that nanoparticles may visualize charge patterns at a sub-60-nm length scale that is not well resolved using KFM.Inorganic, metallic, and semiconducting nanomaterials in the form of nanoparticles, nanowires, nanobelts, or nanodisks are considered key building blocks in the design of novel high-performance nanotechnological devices. The process to fabricate such devices, however, will require new additive concepts to integrate, orient, and assemble such building blocks at desired locations on a substrate. Current approaches that address the integration of nanomaterials at desired locations on a substrate include serial scanning probe based concepts to print 1,2 or manipulate 3 nanomaterials at the sub-100-nm length scale, semiparallel inkjet-based concepts 4,5 to print materials from suspensions with 10 µm scale resolution, parallel nanotransfer methods 6,7 to transfer nanomaterials from one substrate to another retaining a copy of the order, and a vast variety of programmable or "receptor based" assembly concepts 8-18 that use unordered nanomaterials as an input. Scanning probe and inkjet-based methods enable rapid reconfiguration of the patterns and the formation of heterogeneous assemblies at an early stage but remain too slow to print materials over large areas at the sub-10-µm length scale. Nanotransfer concepts are most suitable to transfer nanomaterials from one substrate to another over large areas. Nanotransfer maintains the arrangement of the nanomaterials on a donor substrate; i.e., it does not order or rearrange the materials as part of the process."Receptor"-based concepts focus on the directed assembly of randomly oriented nanomaterials. The materials are suspended in solution or gas phase and are assembled at desired locations (receptors) on a substrate using specific interactions. Most actively investigated areas, currently, use protein recognition, 19,20 DNA hybridization,9,21,22 hydrophobicity/hydrophilicity, surface tension and self-assembled monolayers, 10 topography-directed concepts, 23-25 magnetic 11 and dielectrophoretic assembly and transport, 22,[26][27][28] and electrostatic forces. [12][13][14][15][16][17][18]25,27 In recent years there has been an increased focus on the use of long range electrostatic forces to direct th...

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