Strategies for the improvement of salidroside production in cell suspension cultures of Rhodiola sachalinensis

Xu, J. F.; Liu, C. B.; Han, A. M.; Peng, Feng; Su, Zhiguo

doi:10.1007/s002990050394

Cited by 33 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, salidroside was biosynthesized from tyramine via p-hydroxyphenylacetoaldehyde and tyrosol (p-hydroxyphenylethanol). It has been reported that, in cell suspension cultures of Rhodiola sachalinensis, external tyrosol improved salidroside production [19]. In cultured olive cells, the 13 C-labeled tyramine incorporation into acteoside was also observed, but the incorporation ratio for acteoside was less than that for salidroside.…”

Section: Resultsmentioning

confidence: 88%

Biosynthesis of acteoside in cultured cells of Olea europaea

Saimaru

Orihara

2009

J Nat Med

View full text Add to dashboard Cite

Five phenylethanoid glycosides (acteoside, isoacteoside, beta-oxoacteoside, beta-hydroxyacteoside, and salidroside) were isolated from a cell suspension culture of Olea europaea. We examined the biosynthesis of acteoside in olive cell cultures by using feeding experiments with stable isotope labeled precursors. The hydroxytyrosol moiety of acteoside is biosynthesized from tyrosine through dopamine, whereas the caffeoyl moiety of acteoside is biosynthesized from phenylalanine via a cinnamate pathway. Dopamine is incorporated into acteoside through oxidation to the corresponding aldehyde, reduction to the alcohol, and then beta-glycosylation.

show abstract

Section: Resultsmentioning

confidence: 88%

Biosynthesis of acteoside in cultured cells of Olea europaea

Saimaru

Orihara

2009

J Nat Med

View full text Add to dashboard Cite

show abstract

“…The inoculation with the yeast extract for six days of culture more effectively induced the mitragynine content when compared to twelve days of culture (Figure 5). These differences demonstrate that the biochemical changes that are associated with the induction of secondary metabolism following the addition of elicitors are very complex; it is likely that different metabolic systems can be affected, and there is a large amount of biochemical alteration [28]. In other experiments, the elicitation with all of the salicylic acid treatments did not significantly affect mitragynine production with respect to the control (Figure 6).…”

Section: Resultsmentioning

confidence: 94%

Induction and Analysis of the Alkaloid Mitragynine Content of a Mitragyna speciosa Suspension Culture System upon Elicitation and Precursor Feeding

Zuldin

Said

Noor

et al. 2013

The Scientific World Journal

View full text Add to dashboard Cite

This study aimed to determine the effects of different concentrations and combinations of the phytohormones 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin, 6-benzylaminopurine (BAP), and 1-naphthaleneacetic acid (NAA) on callus induction and to demonstrate the role of elicitors and exogenous precursors on the production of mitragynine in a Mitragyna speciosa suspension culture. The best callus induction was achieved from petiole explants cultured on WPM that was supplemented with 4 mg L−1 2, 4-D (70.83%). Calli were transferred to liquid media and agitated on rotary shakers to establish Mitragyna speciosa cell suspension cultures. The optimum settled cell volume was achieved in the presence of WPM that contained 3 mg L−1 2,4-D and 3% sucrose (9.47 ± 0.4667 mL). The treatment of cultures with different concentrations of yeast extract and salicylic acid for different inoculation periods revealed that the highest mitragynine content as determined by HPLC was achieved from the culture treated with 250 mg L−1 yeast extract (9.275 ± 0.082 mg L−1) that was harvested on day 6 of culturing; salicylic acid showed low mitragynine content in all concentrations used. Tryptophan and loganin were used as exogenous precursors; the highest level of mitragynine production was achieved in cultures treated with 3 μM tryptophan and harvested at 6 days (13.226 ± 1.98 mg L−1).

show abstract

“…This information supports the opinion that salidroside biosynthesis begins with the decarboxylation of Tyr by TyrDC, which produces tyramine. More supporting facts show that the addition of 1 mM Tyr or 1 mM tyrosol increased 9-fold the salidroside content in cell cultures of R. sachalinensis, resulting in the highest ever reported salidroside content by Rhodiola cell suspension culture (154.95 mg/g), while the Phe did not exhibit the same effect (Xu et al 1998b). expressed, but when the competitive pathways and negative regulation were eliminated as well (Bai et al 2014).…”

Section: Para-coumaricmentioning

confidence: 82%