Strategies for the production of cell wall‐deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production

Park, Sang‐Hyuck; Ong, Rebecca G.; Sticklen, Mariam B.

doi:10.1111/pbi.12505

Cited by 35 publications

(22 citation statements)

References 137 publications

(228 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the most interesting groups in the present study includes genes whose expression is only detected in one tissue. This kind of genes and their promoters are of particular interest because they can be used as expression markers and to drive tissue‐specific expression in transformed organisms as illustrated by the production of cell wall‐deconstructing enzymes in planta (Park et al ., ).…”

Section: Discussionmentioning

confidence: 97%

The gene expression landscape of pine seedling tissues

et al. 2017

View full text Add to dashboard Cite

Conifers dominate vast regions of the Northern hemisphere. They are the main source of raw materials for timber industry as well as a wide range of biomaterials. Despite their inherent difficulties as experimental models for classical plant biology research, the technological advances in genomics research are enabling fundamental studies on these plants. The use of laser capture microdissection followed by transcriptomic analysis is a powerful tool for unravelling the molecular and functional organization of conifer tissues and specialized cells. In the present work, 14 different tissues from 1-month-old maritime pine (Pinus pinaster) seedlings have been isolated and their transcriptomes analysed. The results increased the sequence information and number of full-length transcripts from a previous reference transcriptome and added 39 841 new transcripts. In total, 2376 transcripts were ubiquitously expressed in all of the examined tissues. These transcripts could be considered the core 'housekeeping genes' in pine. The genes have been clustered in function to their expression profiles. This analysis reduced the number of profiles to 38, most of these defined by their expression in a unique tissue that is much higher than in the other tissues. The expression and localization data are accessible at ConGenIE.org (http://v22.popgenie.org/microdisection/). This study presents an overview of the gene expression distribution in different pine tissues, specifically highlighting the relationships between tissue gene expression and function. This transcriptome atlas is a valuable resource for functional genomics research in conifers.

show abstract

Section: Discussionmentioning

confidence: 97%

The gene expression landscape of pine seedling tissues

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The use of different promoters in recombinant protein expression systems, in general, has been reviewed extensively [96][97][98][99]. The promoters used in heterologous expression systems vary significantly in their nature, activity, location, and mode of action.…”

Section: Promoters Signal Peptides and Targeted Expressionmentioning

confidence: 99%

“…The promoters used in heterologous expression systems vary significantly in their nature, activity, location, and mode of action. Studies revealed that in plants, the signal peptides and promoters are available to express, guide and store the protein in almost all cellular and extracellular targets [99]. Among the different types of promoters, the constitutive [ One of the major disadvantages, when plants are used as an expression platform, is the plant-specific secondary modifications added on to the proteins, which may make them immunogenic and hence useless in many applications.…”

Section: Promoters Signal Peptides and Targeted Expressionmentioning

confidence: 99%

Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update

Satheeshkumar

2020

Mol Biotechnol

View full text Add to dashboard Cite

Single chain variable fragments (scFvs) are generated by joining together the variable heavy and light chain of a monoclonal antibody (mAb) via a peptide linker. They offer some advantages over the parental mAb such as low molecular weight, heterologous production, multimeric form, and multivalency. The scFvs were produced against more than 50 antigens till date using 10 different plant species as the expression system. There were considerable improvements in the expression and purification strategies of scFv in the last 24 years. With the growing demand of scFv in therapeutic and diagnostic fields, its biosynthesis needs to be increased. The easiness in development, maintenance, and multiplication of transgenic plants make them an attractive expression platform for scFv production. The review intends to provide comprehensive information about the use of plant expression system to produce scFv. The developments, advantages, pitfalls, and possible prospects of improvement for the exploitation of plants in the industrial level are discussed.

show abstract

“…Importantly, we include only enzymes that are active in planta concurrent with cell wall development. The engineering of plants to accumulate hyperthermophilic or inactive cell wall-degrading enzymes has been well reviewed in other works (Mir et al, 2014;Damm et al, 2016;Park et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass

Brandon

Scheller

2020

Front. Plant Sci.

View full text Add to dashboard Cite

Large-scale, sustainable production of lignocellulosic bioenergy from biomass will depend on a variety of dedicated bioenergy crops. Despite their great genetic diversity, prospective bioenergy crops share many similarities in the polysaccharide composition of their cell walls, and the changes needed to optimize them for conversion are largely universal. Therefore, biomass modification strategies that do not depend on genetic background or require mutant varieties are extremely valuable. Due to their preferential fermentation and conversion by microorganisms downstream, the ideal bioenergy crop should contain a high proportion of C6-sugars in polysaccharides like cellulose, callose, galactan, and mixed-linkage glucans. In addition, the biomass should be reduced in inhibitors of fermentation like pentoses and acetate. Finally, the overall complexity of the plant cell wall should be modified to reduce its recalcitrance to enzymatic deconstruction in ways that do no compromise plant health or come at a yield penalty. This review will focus on progress in the use of a variety of genetically dominant strategies to reach these ideals. Due to the breadth and volume of research in the field of lignin bioengineering, this review will instead focus on approaches to improve polysaccharide component plant biomass. Carbohydrate content can be dramatically increased by transgenic overexpression of enzymes involved in cell wall polysaccharide biosynthesis. Additionally, the recalcitrance of the cell wall can be reduced via the overexpression of native or non-native carbohydrate active enzymes like glycosyl hydrolases or carbohydrate esterases. Some research in this area has focused on engineering plants that accumulate cell wall-degrading enzymes that are sequestered to organelles or only active at very high temperatures. The rationale being that, in order to avoid potential negative effects of cell wall modification during plant growth, the enzymes could be activated post-harvest, and post-maturation of the cell wall. A potentially significant limitation of this approach is that at harvest, the cell wall is heavily lignified, making the substrates for these enzymes inaccessible and their activity ineffective. Therefore, this review will only include research employing enzymes that are at least partially active under the ambient conditions of plant growth and cell wall development.

show abstract

Strategies for the production of cell wall‐deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production

Cited by 35 publications

References 137 publications

The gene expression landscape of pine seedling tissues

The gene expression landscape of pine seedling tissues

Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update

Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass

Contact Info

Product

Resources

About