Strategies for Transferring Mixtures of Organic Contaminants from Aquatic Environments into Bioassays

Jahnke, Annika; Mayer, Philipp; Schäfer, Sabine; Witt, Gesine; Haase, Nora; Escher, Beate I.

doi:10.1021/acs.est.5b04687

Cited by 49 publications

(46 citation statements)

References 59 publications

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“…Overall, with additional data of HHOCs, our study validated the hypothesis by Jahnke et al (2016) that partition coefficients between SL and PDMS are largely independent of chemical hydrophobicity, promoting direct use of HOC concentrations in passive samplers for estimating their bioaccumulation potential with average KSL,PDMS value of 23.9 g/g. Moreover, the ML and protein had a weaker affinity for HOCs than SL, and KML,PDMS (0.286-11.8 g/g) and Kpro,PDMS (0.067-2.62 g/g) were also less dependent of hydrophobicity than their partition coefficients from water.…”

Section: Determination Of Kslpdms Kmlpdms and Kpropdmssupporting

confidence: 73%

Determining equilibrium partition coefficients between lipid/protein and polydimethylsiloxane for highly hydrophobic organic contaminants using preloaded disks

Pei

You

2017

Science of The Total Environment

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Bioaccumulation of hydrophobic organic contaminants is of great concern and understanding their partitioning to biological phases is crucial for estimating their bioaccumulation potential. The estimation, however, was of large uncertainty for highly hydrophobic organic contaminants (HHOCs) with log K>9 due to the challenge of quantifying their water concentrations. In the present study, partition coefficients between polydimethylsiloxane (PDMS) and storage lipid (K), membrane lipid (K) and protein (K) were measured for 21 polychlorinated biphenyls (PCBs), 14 polybrominated diphenyl ethers (PBDEs), dechlorane plus (DP) and decabromodiphenyl ethane (DBDPE), covering log K from 5.07 to 11.6, using a preloaded PDMS depletion method. The values of K, K and K were in the ranges of 5.36-52.5, 0.286-11.8 and 0.067-2.62g/g, respectively, being relatively constant although their K values extend more than six orders of magnitude. The relative sorption capacity of the biological phases showed storage lipid was the dominant sorption phase in biota, followed by membrane lipid and protein was the lowest. The K values of the compounds with log K<9 were similar (0.382-14.9g/g) regardless of the thickness of preloaded PDMS disks (58-209μm). For HHOCs, however, K values dropped when thinner PDMS disks were used, as a result of slow diffusion of HHOCs in PDMS. The K values of HHOCs measured by 58-μm PDMS disks ranged from 1.78 to 6.85g/g, which was consistent with compounds with log K<9. This validated that partition coefficients between PDMS and biological phases were independent of chemical hydrophobicity, showing the advantage of using PDMS-based methods to directly estimate bioaccumulation potential of HHOCs.

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Section: Determination Of Kslpdms Kmlpdms and Kpropdmssupporting

confidence: 73%

Determining equilibrium partition coefficients between lipid/protein and polydimethylsiloxane for highly hydrophobic organic contaminants using preloaded disks

Pei

You

2017

Science of The Total Environment

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“…Passive samplers are recognized analytical tools for measuring freely dissolved hydrophobic organic compounds (Mayer et al ). Interest has grown in applying such devices in reverse for passively dosing exposure media in aquatic toxicity tests (Jahnke et al ). Passive dosing has an advantage over traditional dosing methods in maintaining well‐controlled exposures that do not exceed solubility limits of individual or multiconstituent substances.…”

Section: Introductionmentioning

confidence: 99%

Passive dosing yields dissolved aqueous exposures of crude oil comparable to the CROSERF (Chemical Response to Oil Spill: Ecological Effects Research Forum) water accommodated fraction method

Bera

Parkerton

Redman

et al. 2018

Enviro Toxic and Chemistry

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The Chemical Response to Oil Spill: Ecological Effects Research Forum's water accommodated fraction procedure was compared with 2 alternative techniques in which crude oil was passively dosed from silicone tubing or O-rings. Fresh Macondo oil (MC252) was dosed at 30 mg/L using each approach to investigate oil dissolution kinetics, which was monitored by fluorometry as estimated oil equivalents (EOEs). Subsequent experiments with each dosing method were then conducted at multiple oil loadings. Following equilibration, test media were analytically characterized for polyaromatic hydrocarbons (PAHs) using gas chromatography (GC)-mass spectrometry and dissolved oil using biomimetic solid-phase microextraction (SPME). The results showed that equilibrium was achieved within 72 h for all methods. Measured PAH concentrations were compared with oil solubility model predictions of dissolved exposures. The concentration and composition of measured and predicted dissolved PAHs varied with oil loading and were consistent between dosing methods. Two-dimensional GC compositional data for this oil were then used to calculate dissolved toxic units for predicting MC252 oil acute toxicity across the expected range of species sensitivities. Predicted toxic units were nonlinear with loading and correlated to both EOE and biomimetic SPME. Passive dosing methods provide a practical strategy to deliver and maintain dissolved oil concentrations while avoiding the complicating role that droplets can introduce in exposure characterization and test interpretation. Environ Toxicol Chem 2018;37:2810-2819. © 2018 SETAC.

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“…• Next to contamination of water, sediment toxicity [15] and remobilisation during flood events need to be quantified [16]. • There is need to linking exposure and bioassay response [17][18][19]. • As multiple toxicants occur in the environment, their combined effects need to be predicted [20].…”

Section: Biological Effects and Adaptationmentioning

confidence: 99%

20 years SETAC GLB: increasing realism of pesticide risk assessment

Liess

Ratte²,

Ebke

et al. 2019

Environ Sci Eur

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Pesticides contribute to this reduction of biodiversity in ecosystems. Obviously, environmental risk assessment did not prevent adverse pesticide effects on non-target organisms. This called for an identification of processes that are relevant to extrapolate from simplified investigations to the reality of pesticide effects in the field, one of the prominent research areas at the SETAC GLB since two decades. We identify research areas that are relevant to link toxicant effects from test systems with the ecosystem to increase the realism of pesticide risk assessment.

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Strategies for Transferring Mixtures of Organic Contaminants from Aquatic Environments into Bioassays

Cited by 49 publications

References 59 publications

Determining equilibrium partition coefficients between lipid/protein and polydimethylsiloxane for highly hydrophobic organic contaminants using preloaded disks

Determining equilibrium partition coefficients between lipid/protein and polydimethylsiloxane for highly hydrophobic organic contaminants using preloaded disks

Passive dosing yields dissolved aqueous exposures of crude oil comparable to the CROSERF (Chemical Response to Oil Spill: Ecological Effects Research Forum) water accommodated fraction method

20 years SETAC GLB: increasing realism of pesticide risk assessment

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