Strategies for Zinc Uptake in Pseudomonas aeruginosa at the Host–Pathogen Interface

Wang, Shuaitao; Cheng, Jimin; Yanting, Niu; Li, Panxin; Zhang, Xiangqian; Lin, Jie

doi:10.3389/fmicb.2021.741873

Cited by 10 publications

(6 citation statements)

References 122 publications

(216 reference statements)

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“…Protease’s functions could be crucial for both bacterial survival and infection process and targeting bacterial proteases could be a perspective way to combat bacterial infections [50]. Moreover, for Zn proteases, host nutritional immunity (including Zn-dependent processes) was shown to be effective against infections caused by P. aeruginosa [51]. Also, deprivation of Zn ions was proposed to combat infections caused by another common pathogen - S. aureus [52].…”

Section: Discussionmentioning

confidence: 99%

Biofilm matrix proteome of clinical strain of P. aeruginosa

Егорова¹,

Solovyev²,

Polyakov³

et al. 2021

Preprint

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Extracellular matrix plays a pivotal role in biofilm biology. Despite importance of matrix proteins as potential targets for development of antibacterial therapeutics little is known about matrix proteomes. While P. aeruginosa is one of the most important pathogens with emerging antibiotic resistance only few studies are devoted to matrix proteomes and there are no studies describing matrix proteome for any clinical isolates. As matrix responsible for some extracellular functions, it is expected that protein composition should be different in comparison with embedded in biofilm cells and this difference reflects possible active processes in matrix. Here we report the first matrix proteome for clinical isolate of P. aeruginosa in comparison with embedded cells. We have identified the largest number of proteins in matrix among all published studies. Ten proteins were unique for matrix and not present inside cells, but most of these proteins do not have well described function with respect to extracellular component of biofilm. Functional classification of enriched in matrix proteins resulted in several bioprocess groups of proteins. Top three groups were: oxidation-reduction processes, nucleoside metabolism and fatty acid synthesis. Finally, we discuss obtained data in prism of possible directions for antibiofilm therapeutic development.

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Section: Discussionmentioning

confidence: 99%

Biofilm matrix proteome of clinical strain of P. aeruginosa

Егорова¹,

Solovyev²,

Polyakov³

et al. 2021

Preprint

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“…Zinc values of 0.4 ± 0.00 (Mapunda and Mligo, 2019) and 13.6 ± 0.01mg/kg (Bhat et al, 2010) have been reported. Zinc is broadly involved in catalysis, cell structure formation, and regulation of body processes (Wang et al, 2021). Relative Dietary Allowance (RDA) for Zn is 8 mg/day for women and 11 mg/day for men.…”

Section: Climatic Conditionsmentioning

confidence: 99%

Physicochemical and nutritional properties of Syzygium cumini (L.) skeels fruits grown in varied microclimates in Kenya

Rose¹,

John²,

Lydia³

et al. 2023

Afr. J. Pure Appl. Chem.

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Wild fruits contribute significantly to food security, thus becoming an important global discussion. This study evaluated the physicochemical and nutritional properties of Syzygium cumini (L.) Skeels fruits from two microclimates in Kenya as essential contributors to the human diet. Analysis was done using standard methodologies including the use of inductively coupled plasma -optical emission spectrometer for elemental analysis and high-pressure liquid chromatography for the determination of Vitamin C. The T-test showed significant differences in the fruit breadth, pH, total ash, sodium, calcium, manganese, copper, and zinc. The Pearson correlation matrix showed a small positive association between total soluble solids and titratable acidity with altitude, a medium positive correlation with rainfall, and a strong positive correlation between sunshine and skin colour intensity. Larger fruits contained substantial amounts of protein and crude fiber with a significant increase in energy values in fruits with high crude fat and carbohydrates, all correlating positively with the microclimate conditions; altitude, and rainfall. This study exemplifies the potential of Syzygium cumini as an alternative feed supplement to strengthen food security. It provides information on the variation of the physicochemical and nutritional composition of the fruits with climatic conditions, for the industries to employ the best strategies in obtaining marketable products.

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“…Zinc (Zn) is essential to host immune function, and even mild zinc insufficiency leads to widespread defects in both innate and adaptive immunity, resulting in impaired clearance of pathogens. P. aeruginosa have developed zinc uptake systems (ZnuABC, HmtA, and ZrmABCD) which are regulated by QS and specific molecules, such as the zinc uptake regulator (Zur) ( Wang et al, 2021 ). Although Zn uptake systems are not yet clearly understood, to ensure a successful infection, P. aeruginosa must adapt to the environment of zinc deficiency, and this can provide new ideas and methods for the development of new drugs targeting P. aeruginosa zinc uptake systems and corresponding treatments for infection ( Wang et al, 2021 ).…”

Section: Host Interaction and Colonizationmentioning

confidence: 99%

Conquering the host: Bordetella spp. and Pseudomonas aeruginosa molecular regulators in lung infection

2022

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When bacteria sense cues from the host environment, stress responses are activated. Two component systems, sigma factors, small RNAs, ppGpp stringent response, and chaperones start coordinate the expression of virulence factors or immunomodulators to allow bacteria to respond. Although, some of these are well studied, such as the two-component systems, the contribution of other regulators, such as sigma factors or ppGpp, is increasingly gaining attention. Pseudomonas aeruginosa is the gold standard pathogen for studying the molecular mechanisms to sense and respond to environmental cues. Bordetella spp., on the other hand, is a microbial model for studying host-pathogen interactions at the molecular level. These two pathogens have the ability to colonize the lungs of patients with chronic diseases, suggesting that they have the potential to share a niche and interact. However, the molecular networks that facilitate adaptation of Bordetella spp. to cues are unclear. Here, we offer a side-by-side comparison of what is known about these diverse molecular mechanisms that bacteria utilize to counteract host immune responses, while highlighting the relatively unexplored interactions between them.

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Strategies for Zinc Uptake in Pseudomonas aeruginosa at the Host–Pathogen Interface

Cited by 10 publications

References 122 publications

Biofilm matrix proteome of clinical strain of P. aeruginosa

Biofilm matrix proteome of clinical strain of P. aeruginosa

Physicochemical and nutritional properties of Syzygium cumini (L.) skeels fruits grown in varied microclimates in Kenya

Conquering the host: Bordetella spp. and Pseudomonas aeruginosa molecular regulators in lung infection

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