Strategies of nanoparticles integration in polymer fibers to achieve antibacterial effect and enhance cell proliferation with collagen production in tissue engineering scaffolds

Karbowniczek, Joanna; Berniak, Krzysztof; Knapczyk‐Korczak, Joanna; Williams, G. D.; Bryant, Jack A.; Nikoi, Naa Dei; Banzhaf, Manuel; Cogan, Felicity de; Stachewicz, Urszula

doi:10.1016/j.jcis.2023.07.066

Cited by 21 publications

(11 citation statements)

References 52 publications

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“…The electrospinning time was 30 s for all samples. The samples were directly deposited on a 10 × 10 glass wafer/substrate (cover glass, Epredia, USA) for CLSM analyses . The average fiber diameter values were calculated from 100 measurements based on SEM images, and the error was based on the standard deviation.…”

Section: Methodsmentioning

confidence: 99%

“…The samples were directly deposited on a 10 × 10 glass wafer/substrate (cover glass, Epredia, USA) for CLSM analyses. 47 The average fiber diameter values were calculated from 100 measurements based on SEM images, and the error was based on the standard deviation. The fiber diameter was analyzed using ImageJ software (version 1.51, Fiji, USA).…”

Section: Methodsmentioning

confidence: 99%

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Cell–Material Interplay in Focal Adhesion Points

Berniak,

Ura,

Piórkowski

et al. 2024

ACS Appl. Mater. Interfaces

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The complex interplay between cells and materials is a key focus of this research, aiming to develop optimal scaffolds for regenerative medicine. The need for tissue regeneration underscores understanding cellular behavior on scaffolds, especially cell adhesion to polymer fibers forming focal adhesions. Key proteins, paxillin and vinculin, regulate cell signaling, migration, and mechanotransduction in response to the extracellular environment. This study utilizes advanced microscopy, specifically the AiryScan technique, along with advanced image analysis employing the Density-Based Spatial Clustering of Applications with Noise (DBSCAN) cluster algorithm, to investigate protein distribution during osteoblast cell adhesion to polymer fibers and glass substrates. During cell attachment to both glass and polymer fibers, a noticeable shift in the local maxima of paxillin and vinculin signals is observed at the adhesion sites. The focal adhesion sites on polymer fibers are smaller and elliptical but exhibit higher protein density than on the typical glass surface. The characteristics of focal adhesions, influenced by paxillin and vinculin, such as size and density, can potentially reflect the strength and stability of cell adhesion. Efficient adhesion correlates with wellorganized, larger focal adhesions characterized by increased accumulation of paxillin and vinculin. These findings offer promising implications for enhancing scaffold design, evaluating adhesion to various substrates, and refining cellular interactions in biomedical applications.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Cell–Material Interplay in Focal Adhesion Points

Berniak,

Ura,

Piórkowski

et al. 2024

ACS Appl. Mater. Interfaces

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“…In recent years, biomedical textiles have been rapidly developed [ 1 ], and they are extensively being applied in areas such as infection prevention [ 2 ], wound care [ 3 ], and biological tissue scaffolding [ 4 ]. Fibers prepared through electrospinning are considered to be ideal biomedical textiles owing to their large surface area [ 5 ], high porosity [ 6 ], and uniform fiber distribution [ 7 ]. However, their limitations in terms of electrical, thermal, and mechanical performance have hindered the development of fiber membranes via electrospinning.…”

Section: Introductionmentioning

confidence: 99%

Electrospinning Silk-Fibroin-Based Fibrous Membranes with AgNPs for Antimicrobial Application

Li,

Gong,

Jia

et al. 2024

Polymers

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Silk fibroin (SF) has excellent biocompatibility and is one of the most commonly used polymer materials. However, SF fibers have serious drawbacks as antibacterial materials due to their lack of stability and bacterial resistance. Therefore, it is of paramount significance to enhance the stability and bolster the bacterial resistance of SF fibers. In this study, SF fibers were fabricated and loaded with Ag nanoparticles (AgNPs) to improve the antimicrobial properties of the fibers. The impact of reduction conditions on the size of AgNPs was also investigated. In an antibacterial test, the fibers that were prepared exhibited over 98% bacterial resistance against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Therefore, as an efficient antibacterial material, these fibers are expected to become a candidate material in medical and textile fields. This study offers a novel approach for the utilization of SF fibers in the realm of antibacterial applications.

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“…[ 15,16 ] It also effectively improves filler distribution, even at high concentrations, and achieves directional arrangement along polymer fibers. [ 17 ] This addresses a major challenge in fabricating thermally conductive nanocomposites. [ 2,18 ] The fillers' organization during electrospinning directly affects the polymer chains' alignment and reorientation, which increases the thermal conductivity and Young's modulus of produced fibers.…”

Section: Introductionmentioning

confidence: 99%

Bridging a Gap in Thermal Conductivity and Heat Transfer in Hybrid Fibers and Yarns via Polyimide and Silicon Nitride Composites

Moradi

Szewczyk

Stachewicz

2023

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The pressing issues of the energy crisis and rapid electronics development have sparked a growing interest in the production of highly thermally conductive polymer composites. Due to the challenges related to the poor processability of hybrid materials and filler distribution to achieve high thermal conductivity, electrospinning is employed to create composite nanofibers and yarns using polyimide (PI) and thermally conductive silicon nitride (SiN) nanoparticles. The thermal performance of the individual nanofibers is evaluated using scanning thermal microscopy (SThM), providing significant insights into their heat transfer performance. Next, the nanofibers are applied as coatings on resistance wires to assess the thermal conductivity and insulation properties. Notably, the samples containing 35 wt.% of SiN exhibit a 25% increase in surface temperature. These innovative materials hold great promise as exceptional candidates for smart textiles and thermal management applications, addressing the growing demand for effective heat dissipation and regulation.

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Strategies of nanoparticles integration in polymer fibers to achieve antibacterial effect and enhance cell proliferation with collagen production in tissue engineering scaffolds

Cited by 21 publications

References 52 publications

Cell–Material Interplay in Focal Adhesion Points

Cell–Material Interplay in Focal Adhesion Points

Electrospinning Silk-Fibroin-Based Fibrous Membranes with AgNPs for Antimicrobial Application

Bridging a Gap in Thermal Conductivity and Heat Transfer in Hybrid Fibers and Yarns via Polyimide and Silicon Nitride Composites

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