Strategies of stabilization of zein nanoparticles containing doxorubicin hydrochloride

Ambrosio, Nicola; Gagliardi, Agnese; Voci, Silvia; Salvatici, Maria Cristina; Fresta, Massimo; Cosco, Donato

doi:10.1016/j.ijbiomac.2023.125222

Cited by 9 publications

(2 citation statements)

References 48 publications

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“…The zein nanoparticles were characterized by photon correlation spectroscopy using a Zetasizer Nano ZS (Malvern Panalytical Ltd., Spectris plc, England) as previously reported. 18 Mean sizes, polydispersity index (PDI), and the surface charges of the nanoparticles were evaluated following the dilution of each sample in a NaCl 1 mM (1:50 ratio) solution and applying a third-order cumulant fitting correlation function. The results were expressed as the mean of five different experiments and reported as a function of the intensity ± standard deviation.…”

Section: Onepot Procedurementioning

confidence: 99%

“…It is a low-cost biomaterial, insoluble in water yet soluble in organic solvents, such as ethanol, acetone, or acetyl acetone. Due to its versatility, it has been widely employed in the development of micro/nanoparticles, gels, film, and scaffolds with the aim of entrapping both hydrophilic and hydrophobic molecules. − Various preparation approaches have been proposed to obtain zein nanoparticles, with cross-linking, nanoprecipitation, coacervation, and micromixing being the most commonly used techniques. − However, these methods are typically carried out on a small scale and are characterized by batch-to-batch variability. The translation of these procedures into a scaling-up process can be challenging because it is usually hard to obtain large amounts of nanosystems having the same characteristics as a lab-scale batch. − …”

Section: Introductionmentioning

confidence: 99%

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Case Study in the Application of OnePot Prototype Reactor for the Scale-Up of Protein-Based Nanoparticle Production

Romano,

Ambrosio,

Francardi

et al. 2024

ACS Sustainable Chem. Eng.

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Automation is nowadays a preferred way to achieve the efficiency and reproducibility of a chemical process. OnePot is an emerging automated reactor able to ensure reproducibility, scalability, and waste recovery due to its innovative heating technology and sensor system. In this investigation, we demonstrate its applicability to the scaling-up of polymeric nanoparticles. Natural proteins such as zein have emerged as promising raw materials for the development of nanoparticles due to their biocompatibility, safety, and biodegradability. The physicochemical properties and stability of zein-based nanoparticles prepared using different methods, including the innovative OnePot approach, were investigated. The nanoparticles showed mean sizes ranging from ∼130 to 160 nm, a narrow size distribution, and a positive zeta-potential (15 mV). Despite the considerable volume, the OnePot-assisted method demonstrated robustness and efficiency in realizing stable nanoparticles without compromising their physicochemical properties. The evaluation of in vitro toxicity performed on MCF-7 and CaCo-2 cell lines demonstrated no significant differences between the standard nanoprecipitation method and the OnePot-assisted approach. Both formulations were characterized by notable cytotoxicity only at a concentration of 250 μg/mL protein. The comparative analysis showed that the OnePot-assisted method offers substantial time and energy savings compared to conventional procedures, promoting a significant decrease in energy consumption. These findings demonstrated the suitability of the OnePot-assisted method in the development of large amounts of stable zein nanoparticles and also provided evidence of its potential positive impact in the scale-up of various colloidal formulations.

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Section: Onepot Procedurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Case Study in the Application of OnePot Prototype Reactor for the Scale-Up of Protein-Based Nanoparticle Production

Romano,

Ambrosio,

Francardi

et al. 2024

ACS Sustainable Chem. Eng.

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Dual Temperature and pH Sensitive Poly(ethyl methacrylate‐co‐acrylamide) Nanogels Have Potential for Controlled Release of Doxorubicin

Hakkoymaz,

Mazi

2024

ChemistrySelect

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In this study, the synthesis and characterization of poly(ethyl methacrylate‐co‐acrylamide) (i.e., poly(EMA‐co‐AAm)) nanogels and the loading and release studies of doxorubicin hydrochloride were carried out. The synthesis of nanogels was carried out using the emulsion polymerization technique at 60 °C. Swelling experiments showed that the nanogels were sensitive to temperature and pH of the medium. Zeta sizer analysis results showed that the synthesized nanogels had an average size of 23.17 nm and the PDI value was 0.270. In the loading studies of doxorubicin into nanogels, the drug loading capacity (DLC) of the nanogel was determined as 32% and the drug loading efficiency (DLE) was determined as 77%. Release studies conducted at pH = 5.4 and pH = 7.4 at 37 °C showed that the release percentage was 17% even in 32 h at pH = 7.4, and 82% in 5 h at pH = 5.4. Release studies conducted at different temperatures at pH = 5.4 revealed that the release percentage was 82% at 37 °C, 85% at 39 °C, and 89% at 41 °C. These results revealed that poly(EMA‐co‐AAm) nanogels can be used in targeted controlled release of doxorubicin and can increase drug effects while reducing side effects.

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Antioxidant films loaded with β-cyclodextrin-quercetin for enhancing food preservation

Liu,

Wang,

Zhang

et al. 2025

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Strategies of stabilization of zein nanoparticles containing doxorubicin hydrochloride

Cited by 9 publications

References 48 publications

Case Study in the Application of OnePot Prototype Reactor for the Scale-Up of Protein-Based Nanoparticle Production

Case Study in the Application of OnePot Prototype Reactor for the Scale-Up of Protein-Based Nanoparticle Production

Dual Temperature and pH Sensitive Poly(ethyl methacrylate‐co‐acrylamide) Nanogels Have Potential for Controlled Release of Doxorubicin

Antioxidant films loaded with β-cyclodextrin-quercetin for enhancing food preservation

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