Strategies to Ameliorate Oxidative Stress During Assisted Reproduction

Agarwal, Ashok; Durairajanayagam, Damayanthi; Virk, Gurpriya; Plessis, Stefan S. du

doi:10.1007/978-3-319-10259-7

Cited by 7 publications

(5 citation statements)

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“…OS can also negatively influence other sperm components (i.e., nucleic acids and proteins), inducing sperm DNA fragmentation (SDF) and low sperm motility [43]. Furthermore, compared with somatic cells, there is a lack of cytoplasm and poorer antioxidant capacity in mature spermatozoa, thereby rendering it more vulnerable to OS [44]. Nevertheless, it is entirely possible for sperm suffering from oxidative DNA damage to fertilise an oocyte and thus possibly exert adverse effects on the offspring [45], especially in the context of ICSI [46].…”

Section: Paternally Derived Osmentioning

confidence: 99%

Early Life Oxidative Stress and Long-Lasting Cardiovascular Effects on Offspring Conceived by Assisted Reproductive Technologies: A Review

Yang

Kühn

Kolben

et al. 2020

IJMS

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Assisted reproductive technology (ART) has rapidly developed and is now widely practised worldwide. Both the characteristics of ART (handling gametes/embryos in vitro) and the infertility backgrounds of ART parents (such as infertility diseases and unfavourable lifestyles or diets) could cause increased oxidative stress (OS) that may exert adverse influences on gametogenesis, fertilisation, and foetation, even causing a long-lasting influence on the offspring. For these reasons, the safety of ART needs to be closely examined. In this review, from an ART safety standpoint, the origins of OS are reviewed, and the long-lasting cardiovascular effects and potential mechanisms of OS on the offspring are discussed.

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Section: Paternally Derived Osmentioning

confidence: 99%

Early Life Oxidative Stress and Long-Lasting Cardiovascular Effects on Offspring Conceived by Assisted Reproductive Technologies: A Review

Yang

Kühn

Kolben

et al. 2020

IJMS

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“…Second, zinc, folic acid, and selenium promote DNA synthesis and the protamine packaging of sperm chromatin [14]. Finally, carnitine, arginine, and coenzyme Q10 are crucial in transporting fatty acids into mitochondria for energy production [15]. This study examined the efficacy of a combination antioxidant therapy in treating male infertility, employing semen analysis and SDF as outcome measures.…”

Section: Introductionmentioning

confidence: 99%

Micronutrient supplements as antioxidants in improving sperm quality and reducing DNA fragmentation

Nguyen,

Le,

Tran

et al. 2023

Basic Clin. Androl.

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Background Spermatogenesis and sperm quality may be negatively impacted by an increase in reactive oxygen species. This study investigates the efficacy of combined antioxidant therapy for treating male infertility, as measured by semen analyses and the sperm DNA fragmentation index (DFI). Infertile men with a high sperm DNA fragmentation index were instructed to take two oral micronutrient capsules daily for three months. Each antioxidant formulation contained 60 mg vitamin E, 400 µg folic acid, 30 mg selenium, 125 mg L-arginine, 220 mg L-carnitine, 7.5 mg coenzyme Q10, 40 mg L-glutathione, and 20 mg zinc citrate. At entry and post-treatment, the general characteristics, semen analysis, and sperm chromatin dispersion assays were recorded and compared. Results After three months of treatment with antioxidant compounds, the quality of spermatozoa improved significantly, as indicated by a decrease in the mean DNA fragmentation index from 45.6 ± 17.2% to 34.8 ± 20.3%; an increase in sperm concentration from 29.7 × 106/mL to 35.7 × 106/mL (p < 0.001), an increase in a total number of spermatozoa from 72.1 × 106 to 95.5 × 106 (p = 0.012), and an increase in the vitality from 75.5 ± 17.1 to 81.1 ± 14.4% viable forms (p < 0.001). Conclusions Micronutrient supplementation can improve sperm quality and DNA integrity in infertile men. Men with infertility and significant sperm DNA fragmentation who take antioxidants for three months experience a reduction in DNA fragmentation index and an increase in sperm quality as measured by the semen analysis. Trial registration NCT04509583. Registered 12 August 2020, Hue University of Medicine and Pharmacy Ethics Committee—Retrospectively registered.

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“…This, in turn, will provide information on whether the antioxidant is a "fast" acting molecule, that is, provide a fairly rapid therapeutic effect, or will be consumed more slowly and, accordingly, act for a longer time [18]. There are successful strategies for using combinations of "fast" and "slow" antioxidants to ameliorate oxidative stress during assisted reproduction, in the treatment of diabetes and other pathological conditions [19,20]. At the same time, the success is confirmed in both in vivo and kinetic experiments in vitro.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals

Gerasimova,

Salimgareeva,

Magasumova

et al. 2023

Antioxidants

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This work presents a new method using kinetic potentiometry to study the thermodynamic and kinetic parameters of the reactions of antioxidants with peroxyl radicals. The rate constants of the reaction of antioxidants with radicals have been determined, and the groups of “fast” and “slow” antioxidants have been conventionally distinguished. Fast antioxidants include ascorbic, uric, gallic, chlorogenic, caffeic acids, glutathione, L-cysteine, and catechol with constant values from (1.05–9.25) × 103 M·s−1; “slow” antioxidants are α-tocopherol (in aqueous media), ionol, 2,6-ditretbutylphenol, and compounds of the azoloazine series, modified with polyphenolic fragments, with constant values from (4.00–8.50) × 102 M·s−1. It is shown that the value of the rate constant is directly related to the type of kinetic dependence of the potential recorded when an antioxidant is introduced into the solution of the radical initiator. It is shown that the method with the determination of the induction period is difficult in the study of “slow” antioxidants. It has been established that the area above the curve of the kinetic dependence Exp(∆E) is directly related to the amount of inhibited peroxyl radicals and can be used to assess the inhibitory properties of an antioxidant from a thermodynamic point of view. “Fixed time method” and “Initial rate method” were used. Positive correlations between the described method have been established. The utility of the parameter of the area above the curve of the kinetic dependence Exp(∆E) in the study of objects of complex composition is shown.

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Strategies to Ameliorate Oxidative Stress During Assisted Reproduction

Cited by 7 publications

References 0 publications

Early Life Oxidative Stress and Long-Lasting Cardiovascular Effects on Offspring Conceived by Assisted Reproductive Technologies: A Review

Early Life Oxidative Stress and Long-Lasting Cardiovascular Effects on Offspring Conceived by Assisted Reproductive Technologies: A Review

Micronutrient supplements as antioxidants in improving sperm quality and reducing DNA fragmentation

Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals

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