The provision of good treatment can increase various positive things for the growth and development of children with disabilities, one of which is Positive and Negative Reinforcement. This study aims to determine the influence of positive and negative reinforcement on enhancing motivation for basic locomotor movements in children with disabilities. This study uses a pre-experimental, one-group pretest-posttest design. The research was conducted at Kendungkandang State Special School with a sample of 20 students. The data collection technique in this study uses tests and measurements. The results of the study showed a significant influence of Positive Reinforcement on the increase in basic locomotor motivation in children with disabilities. This result was confirmed by a significance value of 0.000 < 0.05. Negative reinforcement also had a significant effect on improving basic locomotor motivation in children with disabilities, with a significance value of 0.000 < 0.05. Although both had a significant influence on improving basic locomotor motivation in children with disabilities, Positive Reinforcement was found to have a stronger effect. This result can be seen from the basic locomotor motion test, where the mean value for Positive Reinforcement was 72.50, while the mean value for Negative Reinforcement was only 63. Therefore, this research concludes that Positive Reinforcement has a greater influence on enhancing basic locomotor motivation in children with disabilities.