Strategies to Reconstruct a Functional Urethral Substitute by Self-assembly Method

Morissette, Amélie; Imbeault, Annie; Cattan, Valérie; Bernard, Geneviève; Taillon, Guillaume; Chabaud, Stéphane; Bolduc, Stéphane

doi:10.1016/j.proeng.2013.05.110

Cited by 6 publications

(7 citation statements)

References 40 publications

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“…Insulin and hypoxia exert a synergic effect on self-assembled tissues. They increase collagen deposition as demonstrated by tests on human and animal cell cultures [101] (unpublished data). Nevertheless, long-term effect of hypoxia exposure (more than 2 weeks) induced acidification of the cell culture medium and a thinning of the engineered tissues [102].…”

Section: Insulin and Hypoxiamentioning

confidence: 83%

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New and Improved Tissue Engineering Techniques: Production of Exogenous Material-Free Stroma by the Self-Assembly Technique

Saba¹,

Jakubowska²,

Chabaud³

et al. 2016

Composition and Function of the Extracellular Matrix in the Human Body

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Tissue engineering results from the use of cells and scaffolds to reproduce structural and spatial organization or function of a tissue. The Production of an ideal engineered tissue depends on its designed purpose. For clinical applications, the main concerns are biocompatibility and the generation of a tissue able to mimic most of its original biological functions. Moreover, the viability of an implanted tissue is associated with its stability to support vascular networks. This chapter summarizes the theory of the self-assembly approach for tissue engineering. Adjustments and modifications in stromal thickness and extracellular matrix composition for various self-assembled tissues are discussed. Methods developed to generate tissue closely mimicking the native morphology and structure, to incorporate capillary-like networks, and to reduce production time and costs are also reviewed. The self-assembly technique leads to the production of a stroma free of exogenous material and can be adapted to generate fastest, inexpensive, and near-tonative tissue bioengineering for medical and fundamental research applications.

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Section: Insulin and Hypoxiamentioning

confidence: 83%

“…Activation of A 2B -adenosine receptors resulted in an increase of collagen synthesis and a decrease in MMP-9 activity [104,105]. This molecule was successfully tested to produce rabbit tissues by the self-assembly approach [101]. Effects of adenosine on human cultures remain to be evaluated.…”

Section: Adenosinementioning

confidence: 99%

New and Improved Tissue Engineering Techniques: Production of Exogenous Material-Free Stroma by the Self-Assembly Technique

Saba¹,

Jakubowska²,

Chabaud³

et al. 2016

Composition and Function of the Extracellular Matrix in the Human Body

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“…All procedures involving animals were approved by the local Research Ethics Committee. Fibroblasts from rabbit dermis were isolated using the same protocol as in Section 2.1 except incubation and culture were maintained at 38.5°C, their physiologic body temperature [ 24 ]. Epithelial cells were not isolated for these experiments.…”

Section: Methodsmentioning

confidence: 99%

Origin of Serum Affects Quality of Engineered Tissues Produced by the Self-Assembly Approach

et al. 2016

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Despite the emergence of serum-free media for cell culture, the use of serum to supplement the culture media is still essential in order to produce engineered urologic tissues using the self-assembly approach, not only for the stromal compartment but also for the uroepithelium. Effects of sera on thickness of these two compartments were measured and quality of the epithelial differentiation was evaluated. For bladder mucosa substitute reconstruction, the use of postnatal sera failed to produce an adequate uroepithelium whereas the fetal sera supplementation did. Postnatal sera also provided thinner stromal compartments than the one obtained using fetal sera, no matter if the fibroblasts from healthy or psoriatic donors were used to reconstruct human skin substitutes.

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“…Therefore, an autologous TE urethra was implanted in a rabbit model, the gold standard animal model to study penile surgery [95,106,107]. Technical adjustments were necessary to replicate results when using rabbit cells [108]. Hence, experimental settings should be altered to include organ-specific mesenchymal cells extracted from the bladder.…”

Section: Translation To Rabbit As An Animal Modelmentioning

confidence: 99%

Surgical Correction of Genitourinary Disorders Using the Self-Assembly Tissue Engineering

Pellerin¹,

Christophe²,

Grammond³

et al. 2019

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Urologic and gynaecologic patients often manifest congenital and/or acquired tissue and organ dysfunctions that require surgical reconstruction to recreate the normal genitourinary system functions. Such reconstruction is still a challenge due to the limited availability of suitable tissues, especially for severe urethral and vaginal replacement. Traditional intervention methods have varying degrees of donor site morbidity or inherent side effects. Interestingly, tissue engineering is a growing field that aims to replace or regenerate these dysfunctional tissues and organs with autologous cells, biomaterials, or a combination of both. Experience gained from tissue engineering suggests that the use of acellular matrices alone is not successful in supporting tissue growth over large surfaces. Cellular constituents need to be isolated, cultured and grown in vitro on scaffolds, then transplanted in vivo, in order to achieve successful three-dimensional tissue regeneration. Biomaterials are the backbone for cell-seeded reconstruction of the genitourinary tissues. Several research teams have explored a different cell culture approach based on self-assembly. This innovative technique relies on the capacity of cells cultured in the presence of ascorbate to secrete and deposit their own extracellular matrix forming a tissue-like substance. The mechanical and physical strength properties of these reconstructed tissues are similar to that of natural native tissues in certain models. Tissue engineered substitutes for urethral and vaginal mucosa were produced and subcutaneously grafted with success opening the way to new therapeutic strategies to correct genitourinary defects.

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Strategies to Reconstruct a Functional Urethral Substitute by Self-assembly Method

Cited by 6 publications

References 40 publications

New and Improved Tissue Engineering Techniques: Production of Exogenous Material-Free Stroma by the Self-Assembly Technique

New and Improved Tissue Engineering Techniques: Production of Exogenous Material-Free Stroma by the Self-Assembly Technique

Origin of Serum Affects Quality of Engineered Tissues Produced by the Self-Assembly Approach

Surgical Correction of Genitourinary Disorders Using the Self-Assembly Tissue Engineering

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