Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

Saxena, Rohit; Rodríguez-Jasso, Rosa M.; Chávez‐González, Mónica L.; Aguilar, Cristóbal N.; Quijano, Guillermo; Ruiz, Héctor A.

doi:10.3390/fermentation8080374

Cited by 17 publications

(6 citation statements)

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“…In corroboration, Cuellar-Bermudez et al [35], found that when Arthrospira is grown under nitrogen-poor conditions, it reduces the protein content and accumulates carbohydrates (up to 57-77% of dry weight), generally glycogen [37]. Similar results have been shared by Saxena et al [38] in A. platensis. Acclimatization to N starvation is probably the best example of complexity in molecular responses.…”

Section: Discussionsupporting

confidence: 59%

Optimal Laboratory Cultivation Conditions of Limnospira maxima for Large-Scale Production

Pineda-Rodríguez,

Herazo-Cárdenas,

Vallejo-Isaza

et al. 2023

Biology

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Cultivating Limnospira maxima, renowned for its abundant proteins and valuable pigments, faces substantial challenges rooted in the limited understanding of its optimal growth parameters, associated high costs, and constraints in the procurement of traditional nitrogen sources, particularly NaNO3. To overcome these challenges, we conducted a comprehensive 4 × 3 factorial design study. Factors considered included white, red, blue, and yellow light spectra, along with nitrogen sources NaNO3 and KNO3, as well as a nitrogen-free control, for large-scale implementation. Optimal growth, measured by Optical Density, occurred with white and yellow light combined with KNO3 as the nitrogen source. These conditions also increased dry weight and Chl-a content. Cultures with nitrogen deprivation exhibited high values for these variables, attributed to carbon accumulation in response to nitrogen scarcity. Phycocyanin, a crucial pigment for nutrition and industry, reached its highest levels in cultures exposed to white light and supplemented with KNO3, with an impressive content of 384.11 g kg−1 of dry weight. These results highlight the efficacy and cost-efficiency of using a combination of white light and KNO3 for large-scale L. maxima cultivation. This strategy offers promising opportunities to address global food security challenges and enhance the production of industrially relevant pigments.

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Section: Discussionsupporting

confidence: 59%

Optimal Laboratory Cultivation Conditions of Limnospira maxima for Large-Scale Production

Pineda-Rodríguez,

Herazo-Cárdenas,

Vallejo-Isaza

et al. 2023

Biology

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“…The chemical composition of Spirulina has been proven to depend on the geographic region, year, season, climatic conditions, environmental factors, cultivation, and processing methods, as well as the strain used in the bioreactor. The significant variability in research results regarding its chemical composition is also due to the diverse cultivation conditions (acidity, temperature, exposure to light, and drying methods) [43][44][45][46]. The chemical composition of 15 strains of Spirulina, cultivated in controlled environments across various countries (Ethiopia, Kenya, Chad, Mexico, USA, India, Peru, Spain), was investigated by Muhling et al [43].…”

Section: Resultsmentioning

confidence: 99%

Metabolomic Profile of Arthrospira platensis from a Bulgarian Bioreactor—A Potential Opportunity for Inclusion in Dietary Supplements

Nikolova,

Gentscheva,

Gyurova

et al. 2024

Life

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The present study aims to elucidate the metabolomic profile of Arthrospira platensis grown in a bioreactor in Bulgaria. The results show that Arthrospira platensis has a high content of mannose, 137.02 mg g−1, and vitamin A (retinol)—10.3 μg/100 g. High concentrations of calcium, sulfur, and zinc distinguish its elemental composition. The freeze-dried powder contained 15.81 ± 0.45% dietary fiber, 50.16 ± 0.25% total protein content, and 1.22 ± 0.11% total fat content. Among the unsaturated fatty acids with the highest content is α-linolenic acid (25.28%), while among the saturated fatty acids, palmitic acid prevails (22.55%). Of the sterols in the sample, β-sitosterol predominated. There is no presence of microcystins LR, RR, YR, and nodularin. Therefore, Arthrospira platensis grown in a Bulgarian bioreactor is safe for use in the pharmaceutical and food industries. Many of the organic compounds found have applications in medicine and pharmacology and play an important role in biochemical processes in the body. Therefore, Arthrospira platensis grown in Bulgaria has a high potential for use as an independent food supplement or in combination with other natural products.

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“…All the analyses were conducted with a p-value < 0.05. The software utilized was developed by Doctor Emilio Olivares-Sáenz from the Agronomy School at the Autonomous University of Nuevo Leon (UANL), San Nicolás de lo Garza, México [21].…”

Section: Discussionmentioning

confidence: 99%

“…The flask was shaken manually four times a day. This inoculum and conditions were used for all the experiments [20,21].…”

Section: Arthrospira Platensis Inoculum Culture Conditionsmentioning

confidence: 99%

Bioprocess Design and Evaluation of Hydrothermal Hydrolysates from Sargassum sp. for Enhancing Arthrospira platensis Growth and Protein Content

Cabello-Galindo,

Rodríguez-Jasso,

Cid-Ibarra

et al. 2024

Processes

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The proliferation of Sargassum biomass in various coastal areas has led to environmental and socio-economic problems. However, due to their unique composition, these biomasses offer versatile applications, prompting research into their potential in third-generation biorefineries. In this study, the hydrothermal processing of Sargassum sp. was evaluated under specific conditions at 190 °C/50 min and 150 °C/30 min. The resulting hydrolysates (liquid phase) were used as alternative culture media for cultivation. Nine treatments for the cultivation of Arthrospira platensis were assessed, varying the concentration of hydrothermal hydrolysates (HH) at 190 °C/50 min: T1 (5% v/v), T2 (10% v/v), and T3 (15% v/v). T4 (5% v/v), T5 (10% v/v), and T6 (15% v/v), maintaining the same HH conditions, and with the addition of 0.7 g/L NaNO3; and treatments T7, T8, and T9 had concentrations of 5%, 10%, and 15% of HH, respectively, at 150 °C/30 min with the addition of 0.7 g/L NaNO3, respectively. Each treatment was inoculated with 15% (v/v) of A. platensis. Growth kinetics were performed by sampling every three days for 24 days. Quantification of soluble proteins was performed for the best conditions of biomass production. The microalgae demonstrated the ability to grow under mixotrophic medium conditions and to utilize the available carbon sources in the culture medium. Treatment 4 has the highest biomass, with an Xmax (g/L) of 1.94 ± 0.06 and a protein production of 24.17 ± 0.86% (w/w). Therefore, this microalgal biomass can be used in the food matrix according to the biorefinery concept.

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Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

Cited by 17 publications

References 50 publications

Optimal Laboratory Cultivation Conditions of Limnospira maxima for Large-Scale Production

Optimal Laboratory Cultivation Conditions of Limnospira maxima for Large-Scale Production

Metabolomic Profile of Arthrospira platensis from a Bulgarian Bioreactor—A Potential Opportunity for Inclusion in Dietary Supplements

Bioprocess Design and Evaluation of Hydrothermal Hydrolysates from Sargassum sp. for Enhancing Arthrospira platensis Growth and Protein Content

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