The presented chapter examines using artificial neural networks as a processing tool in medical research. Normal and pathological muscle signals obtained by the electromyography method were selected for study. The main research directions are the recognition of pathological changes in stimulated electromyography through neural networks, the use of artificial intelligence methods in the diagnosis of diseases through electromyographic signals, the decision in the diagnosis of myographic diseases using the modular architecture of the neural network, applying different optimization methods in calculating the error of the neural network for the diagnosis of neuromuscular diseases, using neural networks in the comparative analysis of optimization methods of electromyographic signal classifiers, and classifying electromyographic signals based on a sequential machine learning model using deep learning methods.