Strategy for optimized use of LC-MS  for determination of the polyphenolic profiles of apple peel, flesh and leaves

Стефова, Марина; Petkovska, Ana; Ugarković, Sonja; Станоева, Јасмина Петреска

doi:10.1016/j.arabjc.2016.12.009

Cited by 10 publications

(8 citation statements)

References 20 publications

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“…Previous studies reported that hyperoside and isoquercitrin are predominant in apple peel, including Ruby S peel. In agreement with earlier studies (Raffa et al, 2017 ; Stefova et al, 2019 ), which identified phenolic compounds in apple peel, pulp, and leaves, quercetin pentoside and kaempferol hexoside were detected in Ruby S apple peel. Quercetin pentoside is believed to be avicularin (quercetin 3-α- l -arabinofuranoside) or guajaverin (quercetin-3- O -arabino-pyranoside) and is known to be present in apples (Sánchez-Rabaneda et al, 2004 ).…”

Section: Resultssupporting

confidence: 93%

Optimization of the ultrasound-assisted extraction of flavonoids and the antioxidant activity of Ruby S apple peel using the response surface method

Park

Cho

Chang³

et al. 2022

Food Sci Biotechnol

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A Box–Behnken Design (BBD) was employed to optimize the extraction of antioxidants from Ruby S apple peel by ultrasound-assisted extraction (UAE). The effect of extraction temperature (20–40 °C), extraction time (15–45 min), and ethanol concentration (50–90%) in water on extraction yield, total phenol content (TPC), total flavonoid content (TFC), and DPPH radical scavenging activity of Ruby S peel extracts (RPEs) were investigated. The optimized extraction conditions that maximized extraction yield, TPC, TFC, and DPPH radical scavenging ability, were temperature 20 °C, extraction time 25.30 min, and ethanol concentration 50%. The validity of designed model was verified, and experimental values obtained under optimum conditions concurred with predicted values. Hyperoside, isoquercitrin, and phloridzin, were among the major flavonoids extracted. Our findings demonstrate the suitability of UAE and RSM for the optimization of Ruby S peel extraction and suggest the potential use of RPEs as bioactive functional materials.

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Section: Resultssupporting

confidence: 93%

Optimization of the ultrasound-assisted extraction of flavonoids and the antioxidant activity of Ruby S apple peel using the response surface method

Park

Cho

Chang³

et al. 2022

Food Sci Biotechnol

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“…In alike manner, while kaemferol-3-glucoside have been identified in the whole apple (peel and flesh) by Serra et al [2010], Stefova et al [2017] have determined kaempferol hexoside and kaempferol pentoside only in the peel of apples.…”

Section: Phenolic Profile Resultsmentioning

confidence: 99%

ORGANIC ACIDS, SUGARS, PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OF Malus floribunda coccinella FRUIT, PEEL AND FLESH

Çoklar¹,

Akbulut²,

Alhassan³

et al. 2018

Acta Sci.Pol.Hortorum Cultus

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Malus floribunda coccinella is a landscape tree that is generally planted for its pinky flowers and small reddish fruits. The red-fleshed fruits, called crab apples, are rich in anthocyanins and are assumed as an environmental pollution material during the fruit bearing season. The aims of this research were to determine the organic acids, sugars, sugar:acid ratio, color, phenolic compounds and antioxidant activity of the fruit and also to identify the phenolic compounds, monomeric anthocyanins content and antioxidant activities in the peel, flesh and whole fruit. Malic acid (25.394 g kg-1 FW) was the main organic acid of the fruit. In fruits, amounts of sucrose, glucose and fructose were found to be 0.497, 0.504 and 4.334 g 100 g-1 FW, respectively. Highest total phenolic concentration and antioxidant activity values were observed in the peel among the fractions, while protocatechuic and cinnamic acids, rutin, isorhamnetin-3-glucoside, quercetin, procyanidin B1, (+)-catechin and cyanidin-3-galactoside were predominant phenolics of the peel. Highest amounts of chlorogenic acid and (-)-epicatechin were determined in the flesh. Cyanidin-3-galactoside concentration in the flesh was approximately half the amount of that in the peel.

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“…However, the addition of organic co-solvents which could improve the solvating power and the yield of the extraction, such as ethanol, methanol and acetone, could be a suitable strategy [70]. Instead, Stefova et al [71] used UAE with a methanol: water mixture for the extraction of polyphenols. Ultrasound can reduce the operating temperature of extraction for thermolabile compounds; moreover, the cavitation process that occurs during sonication causes the rupture of cell walls, thus enhancing solvent contact with available extractable cell material [72].…”

Section: Extractionmentioning

confidence: 99%

“…Moreover, polyphenols stereochemistry, molecular weight, polarity and degree of polymerization could influence compounds retention. However, the reported methods for the separation of phenolics, as well as their glycosides, have been carried out mainly by reverse phase liquid chromatography (RPLC), on silica-based C18bonded phase columns [71,106,110,111]. The average particle diameter of HPLC packings is typically 3-10 µm.…”

Section: Phenolics and Organic Acidsmentioning

confidence: 99%

“…With columns of smaller particle size, a larger number of plates per unit time is provided, with respect to columns with larger particle size [112]. As mobile phase, binary mixtures of aqueous formic acid or acetic acid and acetonitrile (ACN) or methanol (MeOH) as organic modifiers have been employed [71,106,110,111]. Typically, gradient elutions have been preferred, since multiple-step gradients are more suitable for complex mixtures, such as apple extracts.…”

Section: Phenolics and Organic Acidsmentioning

confidence: 99%

See 1 more Smart Citation

Analytical Methods for Extraction and Identification of Primary and Secondary Metabolites of Apple (Malus domestica) Fruits: A Review

Acquavia

Pascale²,

Foti

et al. 2021

Separations

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Apples represent a greater proportion of the worldwide fruit supply, due to their availability on the market and to the high number of existing cultivar varieties and apple-based products (fresh fruit, fruit juice, cider and crushed apples). Several studies on apple fruit metabolites are available, with most of them focusing on their healthy properties’ evaluation. In general, the metabolic profile of apple fruits strongly correlates with most of their peculiar characteristics, such as taste, flavor and color. At the same time, many bioactive molecules could be identified as markers of a specific apple variety. Therefore, a complete description of the analytical protocols commonly used for apple metabolites’ characterization and quantification could be useful for researchers involved in the identification of new phytochemical compounds from different apple varieties. This review describes the analytical methods published in the last ten years, in order to analyze the most important primary and secondary metabolites of Malus domestica fruits. In detail, this review gives an account of the spectrophotometric, chromatographic and mass spectrometric methods. A discussion on the quantitative and qualitative analytical shortcomings for the identification of sugars, fatty acids, polyphenols, organic acids, carotenoids and terpenes found in apple fruits is reported.

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Strategy for optimized use of LC-MS for determination of the polyphenolic profiles of apple peel, flesh and leaves

Cited by 10 publications

References 20 publications

Optimization of the ultrasound-assisted extraction of flavonoids and the antioxidant activity of Ruby S apple peel using the response surface method

Optimization of the ultrasound-assisted extraction of flavonoids and the antioxidant activity of Ruby S apple peel using the response surface method

ORGANIC ACIDS, SUGARS, PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OF Malus floribunda coccinella FRUIT, PEEL AND FLESH

Analytical Methods for Extraction and Identification of Primary and Secondary Metabolites of Apple (Malus domestica) Fruits: A Review

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