Strategy for selecting separation solutions in capillary electrophoresis–mass spectrometry

Preparation and Characterization of a New p-tert-Butyl-calix[8]arene-bonded Stationary Phase for High-Performance Liquid Chromatography

“…1,2 In recent years, the potential of this class of macrocycles has also been shown for several applications in gas chromatography (GC), highperformance liquid chromatography (HPLC) and capillary electrophoresis. [3][4][5][6] The results show that the recognition of calixarenes for many solutes facilitates to improve the separation selectivity. 7 In 1993, Glennon and coworkers [8][9][10] prepared silica-bonded calix [4]arene tetraester and silica-bonded calix [4]arene tetradiethylamide stationary phases to separate metal ions and amino acid esters for the first time.…”

“…Gebauer et al [11][12][13] successfully separated disubstituted aromatics, nucleosides, uracil derivatives, estradiol epimers and cis/trans isomers of proline-containing peptides on calix[n]arene-bonded (n = 4, 5, 6, 8) silica gel. Menyes et al 14 reported that a hexapropylether of p-tert-butyl-calix [6]arene was covalently linked to silica for the separation of PAHs and fullerenes, and showed higher selectivity and lower consumption of the solvent than conventional RP-C18. In the past few years, our research group has prepared p-tert-butyl-calix [6]arene-bonded and p-tert-butylcalix[n]arene-bonded (n = 4, 6, 8) silica-gel stationary phases by a one-pot method with coupling reagents, and investigated the chromatographic separation of some positional isomers, polycyclic aromatic hydrocarbons (PAHs), nucleosides, bases and sulfonamide drugs.…”

“…Menyes et al 14 reported that a hexapropylether of p-tert-butyl-calix [6]arene was covalently linked to silica for the separation of PAHs and fullerenes, and showed higher selectivity and lower consumption of the solvent than conventional RP-C18. In the past few years, our research group has prepared p-tert-butyl-calix [6]arene-bonded and p-tert-butylcalix[n]arene-bonded (n = 4, 6, 8) silica-gel stationary phases by a one-pot method with coupling reagents, and investigated the chromatographic separation of some positional isomers, polycyclic aromatic hydrocarbons (PAHs), nucleosides, bases and sulfonamide drugs. 15,16 The results show that calixarenebonded stationary phases are excellent reverse-phase packings with inclusion capability.…”

“…In our laboratory, Xu et al 15 has prepared the p-tert-butylcalix [6]arene-bonded phase via a breaking ring reaction of 3-glycidoxypropyltrimethoxysilane as a coupling reagent and perchloric acid as a catalyst. The bonded amount of the stationary phase was less than 0.06 mmol g -1 , which could not exhibit ideal separation for the solutes to be tested because of poor selectivity due to a lower bonded amount and column efficiency.…”

A Validated High Performance Liquid Chromatography Method for the Simultaneous Analysis of Guaifenesin, Ambroxol and Loratidine in Bulk and Liquid Dosage form

“…Chemically Guaifenesin is known as 3-(2-Methoxyphenoxy)-1,2-propanediol. Analytical methods for the determination of guaifenesin, either alone or in combination with other drugs, include spectrophotometry (Bhattacharyya et al, 2013;Harika et al, 2012), gas chromatography (Sharaf and Stiff, 2004;Harsono et al, 2005), HPLC (Aluri and Stavchansky, 1993;Amer et al, 2008;Galli and Barbas, 2004;Stavchansky, et al, 1995;Shervington, 1997;Vasudevan et al, 2000;Wilson et al, 1993;Wilcox and Stewart, 2000), capillary electrophoresis-mass spectrometry (Tanaka et al, 1998), X-ray diffraction (Grygar et al, 2008), voltammetry (Tapsoba et al, 2005). Ambroxol hydrochloride (Beeh et al, 2008;Chenot et al, 2014;Gupta, 2010), chemically known as trans-4-((2-amino-3, 5-dibromobenzyl) amino)-cyclohexanol hydrochloride, is a potent mucolytic and mucokinetic, capable of inducing bronchial secretion.…”