Strategy for the separation of strongly polar antioxidant compounds from Lycium barbarum L. via high-speed counter-current chromatography

Wang, Ke; Pei, Dong; Yu, Peiliang; Huang, Xin‐Yi; Zhao, Lichun; Wei, Jianteng; Liu, Jianfei; Di, Duolong

doi:10.1016/j.jchromb.2020.122268

Cited by 10 publications

(1 citation statement)

References 17 publications

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“…Polar compounds have sometimes been considered as potential indicators of the quality of herbal medicines rather than non-polar ones [1]. Besides, they have been increasingly important as herbal drugs for the treatment of chronic diseases [2,3] and as the most important resource for drug discovery [4]. Consequently, the separation of natural polar compounds has gradually become a research hotspot.…”

Section: Introductionmentioning

confidence: 99%

Large‐scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn. by preparative high‐speed counter‐current chromatography

Song

Chen

Wang

et al. 2022

J of Separation Science

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The separation of polar compounds is challenging work due to poor retention and insufficient selectivity. In the present study, an efficient strategy for large‐scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn has been established by preparative high‐speed counter‐current chromatography. Macroporous resin column chromatography was used for the enrichment of the polar polyphenols. However, sugar and other ultra‐polar impurities were co‐washed out with the targets. Liquid‐liquid extraction with ethyl acetate/water (1/1, v/v) solvent system was developed to remove the ultra‐polar impurities with a clearance rate of 95%. Finally, the targets were introduced to preparative high‐speed counter‐current chromatography for separation using ethyl acetate/n‐butanol/acetic acid/water (2/7/1/10, v/v/v/v) solvent system. As a result, 191 mg of Mucic acid 1,4‐lactone 5‐O‐gallate, 370 mg of β‐Glucogallin, 301 mg of Gallic acid, 195 mg of Mucic acid 1,4‐lactone 3‐O‐gallate and 176 mg of Mucic acid 1,4‐lactone 2‐O‐gallate with purity higher than 98% were obtained from 1.5 g of sample. Mucic acid 1,4‐lactone 3‐O‐gallate, Mucic acid 1,4‐lactone 3‐O‐gallate, and Mucic acid 1,4‐lactone 2‐O‐gallate are isomers. The results showed that high‐speed counter‐current chromatography could be well developed for the separation of polar compounds from natural products.

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Section: Introductionmentioning

confidence: 99%

Large‐scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn. by preparative high‐speed counter‐current chromatography

Song

Chen

Wang

et al. 2022

J of Separation Science

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Recent Advances in Screening and Separating Active Components From Natural Products Based on High‐Speed Countercurrent Chromatography

Wang,

Huang,

Zhang

et al. 2024

J of Separation Science

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Natural products play a vital role in human health because people find that they can reduce the incidence of many diseases, such as cancer and cardiovascular diseases. Therefore, researchers have developed many effective techniques for screening and separating active compounds from natural products. High‐speed countercurrent chromatography is a unique unsupported liquid–liquid chromatography technology that has been widely used in the separation of natural products. In this paper, the research progress of screening and separating active components from natural products by combining high‐speed countercurrent chromatography technology with other technologies is reviewed from two aspects: pre‐column activity assay and post‐column activity assay. The purpose of this paper is to provide some theoretical support for the screening and separation of natural active compounds by high‐speed countercurrent chromatography. I hope that the contents summarized in this paper can provide more basis for the development of screening and separation of natural active compounds to realize large‐scale preparation.

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Arsenosugar extracted from algae: Assessment of countercurrent chromatography for isolation

Morales-Rodríguez,

Sahuquillo,

Minguillón

et al. 2024

Analytica Chimica Acta

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Strategy for the separation of strongly polar antioxidant compounds from Lycium barbarum L. via high-speed counter-current chromatography

Cited by 10 publications

References 17 publications

Large‐scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn. by preparative high‐speed counter‐current chromatography

Large‐scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn. by preparative high‐speed counter‐current chromatography

Recent Advances in Screening and Separating Active Components From Natural Products Based on High‐Speed Countercurrent Chromatography

Arsenosugar extracted from algae: Assessment of countercurrent chromatography for isolation

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