Strategy To Enhance the Wettability of Bioacive Paper-Based Sensors

Tian, Junfei; Jarujamrus, Purim; Li, Lizi; Li, Miaosi; Shen, Wei

doi:10.1021/am301699m

Cited by 20 publications

(18 citation statements)

References 23 publications

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“…For hydrophobic surfaces the erythrocyte suspension remained above the paper surface decreasing the surface area between the blood sample and the antibody solution. Treatment of the hydrophobized antibody paper to increase wettability could aid blood sample penetration (Tian et al, 2012 ). Furthermore, the washing step is hindered with liquid unable to flow through the paper, also affecting negatives.…”

Section: Resultsmentioning

confidence: 99%

Activity and Longevity of Antibody in Paper-Based Blood Typing Diagnostics

et al. 2018

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Paper-based diagnostics provide a low-cost, reliable and easy to use mode of blood typing. The shelf-life of such products, however, can be limited due to the reduced activity of reagent antibodies sorbed on the paper cellulose fibers. This study explores the effects of aging on antibody activity for periods up to 12 months on paper and in solution under different aging and drying conditions—air-dried, lyophilized, and kept as a liquid. Paper kept wet with undiluted antibody is shown to have the longest shelf-life and the clearest negatives. Antibody diluted with bovine serum albumin (BSA) protects against the lyophilization process, however, beyond 9 months aging, false positives are seen. Paper with air-dried antibodies is not suitable for use after 1 month aging. These results inform preparation and storage conditions for the development of long shelf-life blood grouping paper-based diagnostics.

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Section: Resultsmentioning

confidence: 99%

Activity and Longevity of Antibody in Paper-Based Blood Typing Diagnostics

et al. 2018

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“…Hence, this method is mostly used when biomolecules need to be released, as in blood typing. [128][129][130][131] 3.1.2. Adsorption of carrier particles: bioactive inks.…”

Section: Physical Methodsmentioning

confidence: 99%

Cellulose: from biocompatible to bioactive material

2014

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International audienceSince the papyri, cellulose has played a significant role in human culture, especially as paper. Nowadays, this ancient product has found new scientific applications in the expanding sector of paper-based technology. Among paper-based devices, paper-based biosensors raise a special interest. The high selectivity of biomolecules for target analytes makes these sensors efficient. Moreover, simple paper-based detection devices do not require hardware or specific technical skill. They are inexpensive, rapid, user-friendly and therefore highly promising for providing resource-limited settings with point-of-care diagnostics. The immobilization of biomolecules onto cellulose is a key step in the development of these sensing devices. Following an overview of cellulose structural features and physicochemical properties, this article reviews current techniques for the immobilization of biomolecules on paper membranes. These procedures are categorized into physical, biological and chemical approaches. There is no universal method for biomolecule immobilization. Thus, for a given paper-based biochip, each strategy can be considered

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“…The initial decrease is likely due to the initial hydration of the substrate with Nile blue A that is insoluble in PBS medium. The wettability of the paper-based sensors has been shown to have a strong correlation to response time (Tian et al 2012).…”

Section: Characterization Of Cellulose-based Heparin Sensor In Phosphate Buffer Saline (Pbs)mentioning

confidence: 99%

A cellulose-based photoacoustic sensor to measure heparin concentration and activity in human blood samples

Jeevarathinam

Pai

Huang

et al. 2019

Biosensors and Bioelectronics

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Heparin is an indispensable drug in anticoagulation therapy but with a narrow therapeutic window, which dictates regular testing and dose adjustment. However, current monitoring tools have a long turnaround time or are operator intensive. In this work, we describe a cellulose-based photoacoustic sensor for heparin. The sensors have a turnaround time of 6 minutes for whole blood samples and 3 minutes for plasma samples regardless of heparin concentration. These sensors have a limit of detection of 0.28 U/ml heparin in human plasma and 0.29 U/ml in whole blood with a linear response (Pearson's r = 0.99) from 0 -2 U/ml heparin in plasma and blood samples. The relative standard deviation was <12.5% in plasma and < 17.5% in whole blood. This approach was validated with heparin-spiked whole human blood and had a linear correlation with the activated partial thromboplastin time (aPTT) (r = 0.99). We then studied 16 sets of clinical samples-these had a linear correlation with the activated clotting time (ACT) (Pearson's r = 0.86, P<0.0001). The photoacoustic signal was also validated against the cumulative heparin dose (Pearson's r = 0.80, P<0.0001). This approach could have applications in bed-side heparin assays for continuous heparin monitoring.

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Strategy To Enhance the Wettability of Bioacive Paper-Based Sensors

Cited by 20 publications

References 23 publications

Activity and Longevity of Antibody in Paper-Based Blood Typing Diagnostics

Activity and Longevity of Antibody in Paper-Based Blood Typing Diagnostics

Cellulose: from biocompatible to bioactive material

A cellulose-based photoacoustic sensor to measure heparin concentration and activity in human blood samples

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