Strategy to Establish Embryo-Derived Pluripotent Stem Cells in Cattle

Abstract

Stem cell research is essential not only for the research and treatment of human diseases, but also for the genetic preservation and improvement of animals. Since embryonic stem cells (ESCs) were established in mice, substantial efforts have been made to establish true ESCs in many species. Although various culture conditions were used to establish ESCs in cattle, the capturing of true bovine ESCs (bESCs) has not been achieved. In this review, the difficulty of establishing bESCs with various culture conditions is described, and the characteristics of proprietary induced pluripotent stem cells and extended pluripotent stem cells are introduced. We conclude with a suggestion of a strategy for establishing true bESCs.

Pluripotent Core in Bovine Embryos: A Review

“…Similarly, Pillai et al [83] enhanced the cellular reprogramming of bovine fibroblasts to biPSCs by forcing expression of OCT4, SOX2, KLF4, and MYC, but they also reported that inhibition of ALK4/5/7 to block TGFβ/activin/nodal signaling together with GSK3β and MEK1/2 supported robust in vitro self-renewal of naive biPSCs. A detailed review of these topics was recently reported [89].…”