Abstract-Darwin glass formed about 800,000 years ago in western Tasmania, Australia. Target rocks at Darwin crater are quartzites and slates (Siluro-Devonian, Eldon Group). Analyses show 2 groups of glass, Average group 1 is composed of: SiO 2 (85%), Al 2 O 3 (7.3%), TiO 2 (0.05%), FeO (2.2%), MgO (0.9%), and K 2 O (1.8%). Group 2 has lower average SiO 2 (81.1%) and higher average Al 2 O 3 (8.2%). Group 2 is enriched in FeO (+1.5%), MgO (+1.3%) and Ni, Co, and Cr. Average Ni (416 ppm), Co (31 ppm), and Cr (162 ppm) in group 2 are beyond the range of sedimentary rocks. Glass and target rocks have concordant REE patterns (La/Lu = 5.9-10; Eu/Eu * = 0.55-0.65) and overlapping trace element abundances. 87 Sr/ 86 Sr ratios for the glasses (0.80778-0.81605) fall in the range (0.76481-1.1212) defined by the rock samples. ε-Nd results range from −13.57 to −15.86. Nd model ages range from 1.2-1.9 Ga (CHUR) and the glasses (1.2-1.5 Ga) fall within the range defined by the target samples. The 87 Sr/ 86 Sr versus 87 Rb/ 86 Sr regression age (411 ± 42 Ma) and initial ratio (0.725 ± 0.016), and the initial 43 Nd/ 144 Nd ratio (0.51153 ± 0.00011) and regression age (451 ± 140 Ma) indicate that the glasses have an inherited isotopic signal from the target rocks at Darwin crater. Mixing models using target rock compositions successfully model the glass for all elements except FeO, MgO, Ni, Co, and Cr in group 2. Mixing models using terrestrial ultramafic rocks fail to match the glass compositions and these enrichments may be related to the projectile.