Stratum corneum ceramide profiles in vitro, ex vivo, and in vivo: characterization of the α-hydroxy double esterified ceramides

Bakar, Joudi; Michael‐Jubeli, Rime; Libong, Danielle; Baillet‐Guffroy, Arlette; Tfayli, Ali

doi:10.1007/s00216-022-04011-9

Cited by 5 publications

(5 citation statements)

References 28 publications

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“…The stratum corneum, the upper layer of the epidermis, consists of corneocytes embedded in a lipid matrix. [1][2][3] It is responsible for the formation and maintenance of skin barrier against water loss and environmental stresses such as ultraviolet (UV) solar radiation. [4][5][6] The protective role of SC against UV is mainly due to the reflection or the absorption of the radiation.…”

Section: Introductionmentioning

confidence: 99%

Skin surface lipids photo‐oxidation: A vibrational spectroscopy study

Assi

Michael‐Jubeli

Jacques

et al. 2023

J Raman Spectroscopy

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Skin surface lipid (SSL) film is a mixture of sebum and epidermal lipids protecting the skin from the effects of the environment such as solar radiation. SSLs are a suitable target and scavenger for all reactive oxygen species (ROS) generated in the skin. In the SSLs, vitamin E has been identified as one of the main antioxidants that can capture ROS. This paper is the third part of a global study that evaluates the effect of solar radiation on the lipid classes that constitute SSLs. In the first two parts, the effect of solar radiation on fatty acids and triglycerides was studied using vibrational spectroscopy. In this part, the effect on the other lipid classes such as squalene (SQ), cholesterol (Chol), and wax ester (WE) (Palmitoleyl Palmitate) was studied and then the behavior of vitamin E under solar radiation was monitored. Finally, SSLs were irradiated and analyzed using vibrational spectroscopy and HT‐GC/MS to respectively follow the oxidation process and to identify the first target of solar radiation in SSLs. When irradiated alone, SQ oxidation process was detectable only after 2430 J/cm2 dose. Similar observations were obtained for vitamin E (after 1620 J/cm2). No changes were observed on the cholesterol and on the wax ester (Palmitoleyl Palmitate). In contrast, when extracted human SSLs film was irradiated, the more marked modifications were observed on SQ. This confirmed the antioxidant role of SQ by scavenging free radicals generated from the oxidation of polyunsaturated fatty acids (PUFA).

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Section: Introductionmentioning

confidence: 99%

Skin surface lipids photo‐oxidation: A vibrational spectroscopy study

Assi

Michael‐Jubeli

Jacques

et al. 2023

J Raman Spectroscopy

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“…The injection volume was 10 µL and the flow rate was set to 1 mL/min. The ceramide fraction, separated by an absorbance of 215 nm, was quantified using an external ceramide standard, and normalized by measuring the protein content in the epidermis and dermis ( Smith et al ., 1981 ; Yano et al ., 1998 ; Groener et al ., 2007 ; Bakar et al ., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Potential Role of Dietary Salmon Nasal Cartilage Proteoglycan on UVB-Induced Photoaged Skin

Lee,

Hong,

Cho

et al. 2024

Biomol Ther (Seoul)

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New supplements with preventive effects against skin photodamage are receiving increasing attention. This study evaluated the anti-photoaging effects of salmon nasal cartilage proteoglycan (SPG), acting as a functional material for skin health. We administered SPG to in vitro and in vivo models exposed to ultraviolet B (UVB) radiation and assessed its moisturizing and anti-wrinkle effects on dorsal mouse skin and keratinocytes and dermal fibroblasts cell lines. These results showed that SPG restored the levels of filaggrin, involucrin, and AQP3 in the epidermis of UVB-irradiated dorsal skin and keratinocytes, thereby enhancing the keratinization process and water flow. Additionally, SPG treatment increased the levels of hyaluronan and skin ceramide, the major components of intercellular lipids in the epidermis. Furthermore, SPG treatment significantly increased the levels of collagen and procollagen type 1 by down-regulating matrix metalloproteinase 1, which play a crucial role in skin fibroblasts, in both in vitro and in vivo models. In addition, SPG strongly inhibited mitogen-activated protein kinase (MAPKs) signaling, the including extracellular signal-regulated kinase, c-Jun N-terminal kinase (JNK), and p38. These findings suggest that dietary SPG may be an attractive functional food for preventing UVB-induced photoaging. And this SPG product may provide its best benefit when treating several signs of skin photoaging.

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“…hydroxyoctadecadienoic acid (HODE), trihydroxyoctadecenoic acid (triHOME), epoxy-hydroxyoctadecenoic acid (epoxy-HOME) species]; although these species may alter the organization of the cornified lipid envelope, their exact role remains undetermined [25 ▪▪ ]. Other recently discovered ceramide species include branched-chain ceramides, potentially derived from long-chain branched-chain fatty acids in sebum lipids, as reported in mouse skin [26], α-hydroxy double-esterified ceramides identified in both reconstructed epidermis and human skin [27], and keto-ceramides found in the skin of ichthyosis patients with a 3-ketodihydrosphingosine reductase (KDSR) mutation [28].…”

Section: Lipids Have Different Roles Throughout the Skinmentioning

confidence: 99%

Current insights into skin lipids and their roles in cutaneous health and disease

Nicolaou

Kendall²

2022

Current Opinion in Clinical Nutrition &Amp; Metabolic Care

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Purpose of reviewThe unique and complex array of cutaneous lipids include essential components of the skin structure and signalling molecules mediating homeostasis and inflammation. Understanding skin lipid biology and metabolism can support our comprehension of health and disease, including systemic conditions with cutaneous involvement.Recent findingsLipids found on the skin surface, produced by both the host and resident microbes, maintain and regulate the skin microbiome and the epidermal barrier, whilst altered contributions from either source can be detrimental to skin health. The unique lipid composition of the epidermal barrier is essential for its function, and recent studies have expanded our understanding of epidermal ceramide production. This has been supported by improved models available for skin research, including organotypic skin models enabling in-vitro production of complex acylceramides for the first time, and model systems facilitating in-silico exploration of the lipid profile changes observed in clinical samples. Studies have revealed further involvement of lipid mediators such as eicosanoids in cutaneous inflammation, as well as immune regulation in both healthy and diseased skin.SummarySkin lipids offer exciting opportunities as therapeutic targets for many conditions, whether through topical interventions or nutritional supplementation.

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Stratum corneum ceramide profiles in vitro, ex vivo, and in vivo: characterization of the α-hydroxy double esterified ceramides

Cited by 5 publications

References 28 publications

Skin surface lipids photo‐oxidation: A vibrational spectroscopy study

Skin surface lipids photo‐oxidation: A vibrational spectroscopy study

Potential Role of Dietary Salmon Nasal Cartilage Proteoglycan on UVB-Induced Photoaged Skin

Current insights into skin lipids and their roles in cutaneous health and disease

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