Strawberry Proanthocyanidins: Biochemical Markers for Botrytis Cinerea Resistance and Shelf-Life Predictability

Hébert, Chantal; Charles, Marie Thérèse; Gauthier, Laurent; Willemot, C.; Khanizadeh, Shahrokh; Cousineau, J.

doi:10.17660/actahortic.2002.567.143

Cited by 40 publications

(38 citation statements)

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“…In contrast, previous studies have shown that flavan-3-ols, such as catechin and epicatechin, and oligomeric proanthocyanidins have inhibitory and antifungal properties towards B. cinerea (Goetz et al 1999;Hébert et al 2002) and other fungi (de Colmenares et al 1998;Veluri et al 2004). Proanthocyanidin oligomers specifically inhibit fungal enzymes such as protein kinase and stillbene oxidase (Polya and Foo 1994;Goetz et al 1999).…”

Section: Discussionmentioning

confidence: 86%

Flavonoid biosynthesis and degradation play a role in early defence responses of bilberry (Vaccinium myrtillus) against biotic stress

et al. 2009

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Bilberry (Vaccinium myrtillus) represents one of the richest flavonoid sources among plants. Flavonoids play variable, species-dependent roles in plant defences. In bilberry, flavonoid metabolism is activated in response to solar radiation but not against mechanical injury. In this paper, the defence reaction and biosynthesis of phenolic compounds of bilberry was studied after infection by a fungal endophyte (Paraphaeosphaeria sp.) and a pathogen (Botrytis cinerea). The defence response of bilberry was faster against the endophyte than the pathogen. All flavonoid biosynthesis genes tested were activated by each infection. Biosynthesis and accumulation of phenolic acids, flavan-3-ols and oligomeric proanthocyanidins were clearly elevated in both infected samples. Infection by the pathogen promoted specifically accumulation of epigallocatechin, quercetin-3-glucoside, quercetin-3-O-α-rhamnoside, quercetin-3-O-(4"-HMG)-R-rhamnoside, chlorogenic acid and coumaroyl quinic acid. The endophyte-infected plants had a higher content of quercetin-3-glucuronide and coumaroyl iridoid. Therefore, accumulation of individual phenolic compounds could be specific for each infection. Quantity of insoluble proanthocyanidins was the highest in control plants, suggesting that they might act as storage compounds and become activated by degradation upon infection.

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Section: Discussionmentioning

confidence: 86%

Flavonoid biosynthesis and degradation play a role in early defence responses of bilberry (Vaccinium myrtillus) against biotic stress

et al. 2009

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“…The different isolates of B. cinerea were grown in 2% potato dextrose agar (Hébert et al, 2002). Spores from each isolate were taken, resuspended altogether in sterile water and placed on a Petri plate containing the same culture media.…”

Section: Culture Mediamentioning

confidence: 99%

Genetic diversity in Chilean strawberry (Fragaria chiloensis): differential response to Botrytis cinerea infection

González

Moya

Sandoval

et al. 2009

Span. j. agric. res.

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Botrytis cinerea Pers. is a necrotrophic and polyphageous pathogen economically important on soft fruits and crops producing severe agricultural losses. The Chilean strawberry, Fragaria chiloensis (L.) P. Mill. is one of the wild parents of commercial strawberry and has the capacity to tolerate the infection from the pathogenic fungus. A study was performed to make a genetic analysis of wild and cultivated individuals collected from three different regions of Central South Chile and compare the Botrytis infection performance. The dominant ISSR markers were used to generate the genetic fingerprinting and the native samples were contrasted against the commercial Fragaria × ananassa Duch. The samples from Contulmo presented the lowest diversity within population and the highest diversity between populations, but at the same time showed the highest tolerance to Botrytis infection. Infected leaves and fruits from F. × ananassa showed 3.6% of the area covered by the fungus, three days after inoculation, while the plants from Contulmo showed 3.3% of the area covered by the pathogen, five days after inoculation. The plants from Contulmo were classified as highly tolerant (HT), tolerant (T), moderately tolerant (MT), and susceptible (S) where 50% of plants belonged to the group of tolerant. The results, highly significant (p ≤ 0.01), indicate that the samples of F. chiloensis from Contulmo are more genetically homogeneous and can be classified as tolerant to the infection by Botrytis cinerea.Additional key words: grey mould, native strawberry, resistance. ResumenDiversidad genética en frutilla chilena (Fragaria chiloensis): respuesta diferencial a la infección con Botrytis cinerea Botrytis cinerea Pers. es un patógeno necrótrofo y polífago de importancia económica en cultivos y frutales, ocasionando importantes pérdidas en agricultura. La frutilla chilena, Fragaria chiloensis (L.) P. Mill., antecesor silvestre de la frutilla comercial, tiene la capacidad de tolerar la infección de una serie de hongos patogénicos. En este estudio se efectuó un análisis genético de plantas de frutilla chilena silvestres y cultivadas, recolectadas en tres diferentes regiones de la zona centro sur de Chile, en cuanto a su comportamiento frente a la infección de Botrytis. Los marcadores dominantes ISSR fueron utilizados para generar el patrón genética y las muestras de frutilla chilena fueron comparadas con las de Fragaria × ananassa Duch. Las muestras de Contulmo mostraron la más baja diversidad dentro de la población y la mayor entre poblaciones. Al mismo tiempo presentaron la mayor tolerancia a la infección con Botrytis. Las hojas y frutos de F. × ananassa, mostraron, tres días después de la inoculación, un 3,6% de su área cubierta por el patógeno. Por el contrario en las plantas recolectadas en Contulmo la infección fue más lenta, observándose un 3,3% del área cubierta por el patógeno, cinco días después de la inoculación. Las plantas de Contulmo fueron clasificadas como altamente tolerantes, tolerantes, moderadamente tolerantes y...

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“…Hébert et al (2002) reported a better resistance level to grey mold growth using extracts of 6 strawberry cultivars, but it did not mention which were the phenolic compounds or mixes responsible for the inhibition of B. cinerea growth. As for conclusions by Tao et al (2010), in this exercise, we found that there is a negative correlation between shelf life and phenolic compounds (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have concluded that the antioxidant properties of constitutive and induced phenolic compounds provide good defence plant tissue against pathogen intrusions (Nicholson & Hammerschmidt, 1992;Prusky, 1996). Gosselin (2001), andHébert et al (2002) used a similar method to that of Jersch et al (1989) to find out that aqueous extracts of the unriped fruit of 'Chandler' had an unequivocal antifungal action against B. cinerea mycelial growth and conidial germination. In strawberry cultivars, the main phenolic components having antioxidant properties are: ellagic and gallic acids, anthocyanins (cyanidin-3-glucoside, pelargonidin-3-glucoside), catechin, and flavonols (quercetin-3-galactoside) which confers a better resistance to B. cinerea.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, grey mold is the number one destructive disease for strawberry, all around the world (Sutton, 1998). There are many cultural practices which can prevent its growth, including fungicide applications, temperature and atmosphere control, better irrigation and wetness, at different growth stages and postharvest, but the best method still is the selection of cultivars with a greater resistance to its damaging effects (Hébert et al, 2002;Terry, Joyce, Adikaram, & Khambay, 2004). In view of results obtained through many experiments, researchers have attributed variations in the inherent natural disease resistance of strawberry to its skin strength (Gooding, 1976), firmness (Barritt, 1980), flower susceptibility (Bristow, Campbell, Papendick, & Elliot, 1986), raised neck, and reflexed calyx.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of Phenolic Components and Their Antioxidant Capacity in Strawberries

Ying¹,

Charles²,

Dong³

et al. 2014

JFR

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Total phenolic content, phenolic composition, and total antioxidant capacity of neck and bottom parts of two strawberry cultivars, 'Chambly' and 'LL0220-10', were evaluated using the Folin-Ciocalteu method, high performance liquid chromatography, and ferric reducing/antioxidant power, respectively. A significant interaction (p < 0.05) was observed between examined parts and cultivars. The highest content of phenolics was detected in the bottom part of 'Chambly' when compared to its neck, while there was no difference between neck and bottom of 'LL0220-10'. The most evident group was the anthocyanins accounting for 83.53% of the total phenolic content. These data are a step in establishing a correlation between fruit anatomy and its chemical composition, which could be useful in breeding new strawberry cultivars more resistant to diseases.

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Strawberry Proanthocyanidins: Biochemical Markers for Botrytis Cinerea Resistance and Shelf-Life Predictability

Cited by 40 publications

References 0 publications

Flavonoid biosynthesis and degradation play a role in early defence responses of bilberry (Vaccinium myrtillus) against biotic stress

Flavonoid biosynthesis and degradation play a role in early defence responses of bilberry (Vaccinium myrtillus) against biotic stress

Genetic diversity in Chilean strawberry (Fragaria chiloensis): differential response to Botrytis cinerea infection

Distribution of Phenolic Components and Their Antioxidant Capacity in Strawberries

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