StREM1.3 REMORIN Protein Plays an Agonistic Role in Potyvirus Cell-to-Cell Movement in N. benthamiana

Rocher, Marion; Simon, Vincent; Jolivet, Marie-Dominique; Sofer, Luc; Deroubaix, Anne-Flore; Mongrand, Sébastien; German-Retana, Sylvie

doi:10.3390/v14030574

Cited by 16 publications

(25 citation statements)

References 58 publications

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“…However, Arabidopsis AtREM1.2 negatively regulates the cell-to-cell movement of TuMV via competition with PCaP1 for binding actin filaments (Cheng et al, 2020). This negative effect of AtREM1.2 on potyvirus movement contradicts the positive effect observed for StREM1.3 (Rocher et al, 2022), adding another level of complexity concerning the functions of REMORINs in viral infections.…”

Section: Do Ck Ing Of P Ot Y Vir Al " Movement Protein S" At the Pd L...mentioning

confidence: 96%

The mystery remains: How do potyviruses move within and between cells?

Xue

Marissal-Arvy

German-Retana

2023

Molecular Plant Pathology

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The genus Potyvirus is considered as the largest among plant single‐stranded (positive‐sense) RNA viruses, causing considerable economic damage to vegetable and fruit crops worldwide. Through the coordinated action of four viral proteins and a few identified host factors, potyviruses exploit the endomembrane system of infected cells for their replication and for their intra‐ and intercellular movement to and through plasmodesmata (PDs). Although a significant amount of data concerning potyvirus movement has been published, no synthetic review compiling and integrating all information relevant to our current understanding of potyvirus transport is available. In this review, we highlight the complexity of potyvirus movement pathways and present three potential nonexclusive mechanisms based on (1) the use of the host endomembrane system to produce membranous replication vesicles that are targeted to PDs and move from cell to cell, (2) the movement of extracellular viral vesicles in the apoplasm, and (3) the transport of virion particles or ribonucleoprotein complexes through PDs. We also present and discuss experimental data supporting these different models as well as the aspects that still remain mostly speculative.

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Section: Do Ck Ing Of P Ot Y Vir Al " Movement Protein S" At the Pd L...mentioning

confidence: 96%

The mystery remains: How do potyviruses move within and between cells?

Xue

Marissal-Arvy

German-Retana

2023

Molecular Plant Pathology

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“…(Lee and Hwang, 2005; Lee et al, 2011; Zavaliev and Epel, 2015)), while in others aniline blue was introduced into living tissues without fixation (e.g. (Caillaud et al, 2014; Muller et al, 2022; Rocher et al, 2022). Because of these disparate protocols, potential issues arise when comparing studies that use different staining approaches.…”

Section: Main Textmentioning

confidence: 99%

Reliable detection and quantification of plasmodesmal callose inNicotiana benthamianaleaves during defense responses

Sankoh,

Adjei,

Roberts

et al. 2023

Preprint

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Callose, a beta-(1,3)-D-glucan polymer, is essential for regulating intercellular trafficking via plasmodesmata (PD). Pathogens manipulate PD-localized proteins to enable intercellular trafficking by removing callose at PD, or conversely by increasing callose accumulation at PD to limit intercellular trafficking during infection. Plant defense hormones like salicylic acid regulate PD-localized proteins to control PD and intercellular trafficking during innate immune defense responses such as systemic acquired resistance. Measuring callose deposition at PD in plants has therefore emerged as a popular parameter for assessing the intercellular trafficking activity during plant immunity. Despite the popularity of this metric there is no standard for how these measurements should be made. In this study, three commonly used methods for identifying and quantifying PD callose by aniline blue staining were evaluated to determine the most effective in theNicotiana benthamianaleaf model. The results reveal that the most reliable method used aniline blue staining and fluorescent microscopy to measure callose deposition in fixed tissue. Manual or semi-automated workflows for image analysis were also compared and found to produce similar results although the semi-automated workflow produced a wider distribution of data points.

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“…Studies on the molecular mechanism underlying the regulation of callose during virus infection identified REMORIN1.3 (a plant protein that localizes at lipid rafts and plasmodesmata) and a response dependent on the abscisic acid and salicylic acid signaling pathways. Ectopic expression of REMORIN1.3 in tobacco increases plasmodesmata‐callose and affects virus infection, although this response varies such that the spreading of tobamoviruses (e.g., TMV) are restricted, but potyviruses (e.g., Turnip mosaic virus or Potato virus A) are enhanced (Rocher et al, 2022 ). The potyviral movement protein cylindrical inclusion was found to interact with REMORIN1.3 counteracting its role in promoting callose at plasmodesmata.…”

Section: The Role Of Plasmodesmata‐associated Callose In Plant Symbio...mentioning

confidence: 99%

Callose metabolism and the regulation of cell walls and plasmodesmata during plant mutualistic and pathogenic interactions

German

Yeshvekar

Benitez‐Alfonso

2022

Plant Cell & Environment

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Cell walls are essential for plant growth and development, providing support and protection from external environments. Callose is a glucan that accumulates in specialized cell wall microdomains including around intercellular pores called plasmodesmata. Despite representing a small percentage of the cell wall (~0.3% in the model plant Arabidopsis thaliana), callose accumulation regulates important biological processes such as phloem and pollen development, cell division, organ formation, responses to pathogenic invasion and to changes in nutrients and toxic metals in the soil. Callose accumulation modifies cell wall properties and restricts plasmodesmata aperture, affecting the transport of signaling proteins and RNA molecules that regulate plant developmental and environmental responses. Although the importance of callose, at and outside plasmodesmata cell walls, is widely recognized, the underlying mechanisms controlling changes in its synthesis and degradation are still unresolved. In this review, we explore the most recent literature addressing callose metabolism with a focus on the molecular factors affecting callose accumulation in response to mutualistic symbionts and pathogenic elicitors. We discuss commonalities in the signaling pathways, identify research gaps and highlight opportunities to target callose in the improvement of plant responses to beneficial versus pathogenic microbes.

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StREM1.3 REMORIN Protein Plays an Agonistic Role in Potyvirus Cell-to-Cell Movement in N. benthamiana

Cited by 16 publications

References 58 publications

The mystery remains: How do potyviruses move within and between cells?

The mystery remains: How do potyviruses move within and between cells?

Reliable detection and quantification of plasmodesmal callose inNicotiana benthamianaleaves during defense responses

Callose metabolism and the regulation of cell walls and plasmodesmata during plant mutualistic and pathogenic interactions

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