It is crucial to optimize energy consumption in buildings while considering thermal comfort. The first step here involved an EnergyPlus simulation on a trade center building located in Tehran, Bandar Abbas, and Tabriz, Iran. A multi-objective optimization was then performed based on non-dominated sorting genetic algorithm II (NSGA-II) in jEPlus + EA to establish the building in the selected city where would benefit the most from implementing the radiant ceiling cooling system. Efforts were undertaken to choose environmentally-friendly materials. The final solution by Pareto charts resulted in a 52% reduction in energy consumption, a 37.3% decrease in cooling load, and a 17.4% improvement in comfort hours compared to the original design. Annual emission of greenhouse gas reduced as 167.67 tone of CO2 equivalent emission, 25.77 ton of CH4, and 0.2 ton of NO2. The mentioned algorithm was conducted for the first time on a trade center, including a DOAS system and radiant ceiling cooling system. Simultaneously, the environmental-friendly materials were dealt with. The procedure holds significant relevance for the design and optimization of buildings in Iran, especially wherever the climate is hot and humid. This approach offers advantages to the environment by reducing the impact on energy resources and utilizing environmentally-friendly materials.