This book chapter delves into the field of colorimetric analysis of bloodstains in forensic science, focusing on its application in crime scene investigation. Therefore it provides a comprehensive overview of the biological background of age-induced color changes. The chapter begins with an introduction to the significance of blood evidence in solving crimes and the emergence of colorimetry as a valuable tool in blood analysis. The principles of forensic spectroscopy are explored, specifically its ability to provide information crucial to crime reconstruction, such as the age of bloodstains. The chapter discusses the transformation of hemoglobin derivatives over time and the corresponding measurable color changes that accompany aging blood traces.