Streptavidin-Conjugated Oxygen-Doped Single-Walled Carbon Nanotubes as Near-Infrared Labels for Immunoassays

Abstract

Single-walled carbon nanotubes (CNTs) are promising candidates for near-infrared (NIR) fluorescent labels in diagnostic fields. We report a complex of oxygen-doped CNT (o-CNT) and streptavidin (SA) for preparing CNT-based NIR labels with a high reaction efficiency in immunoassays. This complex specifically binds to biotin molecules by conjugating a linker molecule of phospholipid polyethylene glycol (PL-PEG) to SA (o-CNT-SA). The immunoprecipitation reaction efficiency between o-CNT-SA and biotin reaches 79.3% when the surface of o-CNTs is uniformly covered with SA-conjugated PL-PEG. The strong affinity between SA and biotin is useful for preparing CNTbased sensitive NIR fluorescent labels.

Bisphenol-A–Carbon Nanotube Nanocomposite: Interfacial DFT Prediction and Experimental Strength Testing

“…The multi-walled CNTs and acetone-functionalized CNTs (A-CNTs) have become a highly potent candidate to be used as the filler in composite materials owing to their high aspect ratio, high active surface area, and extraordinary mechanical characteristics. The CNTs have been used for various applications in potent areas such as rubber/polymer/metal composites, − sensing, , biomedical, catalysts, and energy storage and strength. − The nanocomposites with CNT reinforcement yield superior specific properties like high strength-to-weight and high stiffness-to-weight ratios. However, CNTs have strong tendency to form aggregates in solution.…”