Streptococcus agalactiae in pregnant women: Phenotypic and genotypic characters

Savoia, Dianella; Gottimer, C.; Crocilla, C.; Zucca, Mario

doi:10.1016/j.jinf.2007.11.007

Cited by 42 publications

(39 citation statements)

References 32 publications

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“…This was the motivation for this study involving Even considering the different rates of GBS infection according to the methodological approach used in this study, 9.5% and 32.6%, both values of prevalence are in agreement with literature data, which point to a prevalence range of 3-41% (13,26,27,32,33,35). This worldwide variability is related to different sociocultural, geographic, climatic, biological and methodological determinants.…”

Section: Discussionsupporting

confidence: 82%

“…Nine distinct serotypes are recognized as part of the human microbiota colonizing mucous membranes, especially the gastrointestinal and genitourinary tracts (7,27). In the 70`s GBS was recognized as the main etiology of early-onset neonatal sepsis, with evidence pointing to vertical transmission (mother-to-infant), chiefly by contact with and aspiration of vaginal secretions from the colonized birth canal during labor (21,28).…”

Section: Lancefield Group B Streptococcus (Gbs) Ormentioning

confidence: 99%

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Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility patterns

Castellano-Filho¹,

Silva²,

Nascimento³

et al. 2010

Braz. J. Microbiol.

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Group B Streptococcus (GBS) is still not routinely screened during pregnancy in Brazil, being prophylaxis and empirical treatment based on identification of risk groups. This study aimed to investigate GBS prevalence in Brazilian pregnant women by culture or polymerase chain reaction (PCR) associated to the enrichment culture, and to determine the antimicrobial susceptibility patterns of isolated bacteria, so as to support public health policies and empirical prophylaxis. After an epidemiological survey, vaginal and anorectal specimens were collected from 221 consenting laboring women. Each sample was submitted to enrichment culture and sheep blood agar was used to isolate suggestive GBS. Alternatively, specific PCR was performed from enrichment cultures. Antimicrobial susceptibility patterns were determined for isolated bacteria by agar diffusion method. No risk groups were identified. Considering the culture-based methodology, GBS was detected in 9.5% of the donors. Twenty five bacterial strains were isolated and identified. Through the culture-PCR methodology, GBS was detected in 32.6% specimens. Bacterial resistance was not detected against ampicillin, cephazolin, vancomycin and ciprofloxacin, whereas 22.7% were resistant to erythromycin and 50% were resistant to clindamycin. GBS detection may be improved by the association of PCR and enrichment culture. Considering that colony selection in agar plates may be laboring and technician-dependent, it may not reflect the real prevalence of streptococci. As in Brazil prevention strategies to reduce the GBS associated diseases have not been adopted, prospective studies are needed to anchor public health policies especially considering the regional GBS antimicrobial susceptibility patterns.

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Section: Discussionsupporting

confidence: 82%

Section: Lancefield Group B Streptococcus (Gbs) Ormentioning

confidence: 99%

Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility patterns

Castellano-Filho¹,

Silva²,

Nascimento³

et al. 2010

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…This is in line with previous findings in the Asia, Europe and the United States (Table 1 [ [6][7][8][9][10][11][12][13][14][15][16][17][18][19]). In the study by Morozumi et al [2], 7/32 (21·9%) serotype Ia and 24/88 (27·3%) serotype III GBS isolates showed ML resistance.…”

Section: Association Between Capsular Serotype V and Macrolide Resistsupporting

confidence: 93%

Association between capsular serotype V and macrolide resistance in group B Streptococcus

Sendi

Fröhlicher

2014

Epidemiol. Infect.

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“…[4,5] To treat GBS infection, penicillin, ampicillin, and cefazolin are the drugs of choice; for patients allergic to penicillin or cephalosporins, vancomycin, macrolides (such as erythromycin, azithromycin, and clarithromycin), and lincosamides (clindamycin) may be used as the alternative drugs. [6][7][8][9] Vancomycin resistance has not been reported in GBS.…”

Section: S Treptococcus Agalactiae (Group B Streptococcus Gbs)mentioning

confidence: 99%

Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan

Wang¹,

Chen²,

Hou³

et al. 2015

Biomed J

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Background: Antimicrobial resistance of Streptococcus agalactiae (Group B Streptococcus, GBS) has been emerging worldwide. We aimed to examine the correlation of drug-resistant genes with serotypes and with the mutations of the quinolone resistance-determining region (QRDR) in GBS isolates. Methods:A total of 323 human GBS isolates were collected from a hospital in southern Taiwan. Laboratory investigation included serotyping by a multiplex polymerase chain reaction (PCR) method, antimicrobial susceptibility testing by a disc diffusion method, and mechanism analysis of the resistance to macrolides and fluoroquinolones by PCR and sequencing methods. Results:Multiplex PCR showed that the most prevalent serotypes were Ib, III, V, and VI, mostly isolated from urine. The ermB gene was highly prevalent in serotypes Ib and V and was associated with clindamycin and macrolide resistance. GBS with a serine-to-leucine mutation at codon 81 in GyrA and with a serine-to-phenylalanine or -tyrosine mutation at codon 79 in ParC had a higher minimum inhibitory concentration of levofloxacin than isolates with only an aspartic acid-to-tyrosine mutation at codon 83 (>32 μg/ml vs. 16 μg/ml) in GyrA. Conclusions:The most prevalent GBS serotypes were Ib, III, V, and VI. The ermB and mefE genes carried in serotypes Ib and V were highly associated with the resistance to macrolides and clindamycin. Mutations at codon 79 and codon 83 of ParC were the major determining factors for high-level fluoroquinolone resistance. (Biomed J 2015;38:215-220)

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Streptococcus agalactiae in pregnant women: Phenotypic and genotypic characters

Cited by 42 publications

References 32 publications

Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility patterns

Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility patterns

Association between capsular serotype V and macrolide resistance in group B Streptococcus

Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan

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