Streptococcus agalactiae INFECTION ON TILAPIA (Oreochromis niloticus) IN CIRATA RESERVOIR, WEST JAVA

Abstract

Streptococcosis is one of bacterial diseases in the culture of Tilapia, Oreochromis niloticus and has caused significant economic losses. Streptococcus iniae, is known as pathogen to marine and freshwater fishes whereas Streptococcus agalactiae is known as pathogen to Tilapia. The isolation and characterization of four isolates of S. agalactiae, were described from an infected Tilapia from Cirata Reservoir, West Java, in July 2008. Conventional and rapid identification systems were used to determine isolates of S. agalactiae from brain and kidney tissues. In this paper, we have characterized S. agalactiae and this was the first isolation of this bacteria from fish. The isolates were gram positive, catalase-negative, oxidase-negative,  haemolytic cocci colonies on blood agar. All of the of isolates were biochemically characterized with the API 20 Strep System (bioMerieux). Bacterial chromosomal DNA used in PCR assay was extr acted by heating method. The forward primer is Sdi 61: 5'-AGGAAACCTGCCATTTGCG-3' and the r ever se pri mer is Sdi 252: 5'-CAATCTATTTCTAGATCGTGG-3' with gene target 16S intergenic spacer and it has 192 bp in length. These primers were designed by Alpha DNA (Montreal, Quebec). The biochemical patterns of four isolates were rather different although almost all traits were similar with the exception of pyroglutamic acid (pyra) and L-arginin (ADH), for which we observed negative and positive reaction in this study. Therefore, some of the biochemical characteristics of the four isolates did not fit 100% with the typical patterns of S. agalactiae. However, the PCR result showed that this PCR assay is an effective tool for rapid and specific detection of S. agalactiae, the main pathogens involved in warm-water streptococcosis, obtained from pure culture of naturally infected fish. Therefore, it could be a useful alternative for culture-based routine diagnosis of warm-water streptococcal infections in fish.

The occurrence ofStreptococcus agalactiaesequence type 261 from fish disease outbreaks of tilapiaOreochromis niloticusin Indonesia

“…S. agalactiae has subsequently been reported from wild and captive fish involved in epizootics in various countries such like USA, Israel, Kuwait, Thailand and Brazil (Plumb, Schachte, Gaines, Peltier & Carrol ; Baya, Lupiani, Hetrick, Roberson, Lukacovic, May & Poukish ; Eldar, Bejerano & Bercovier ; Duremdez, Al‐Marzouk, Qasem, Al‐herbi & Gharabally ; Salvador, Muller, de Freitas, Leonhadt, Pretto‐Giordano & Dias ; Suanyuk, Kanghear, Khongpradit & Supamattaya ). Recently, an S. agalactiae outbreak in tilapia ( Oreochromis niloticus ) was reported in Indonesia (Lusiastuti, Gardenia, Mufidah, and Aryati ; Lusiastuti, Taukhid, and Hadie ).…”

Inovasi Pengembangan Vaksin untuk Budidaya Ikan Air Tawar Berkelanjutan

“…Streptococcus agalactiae diidentifikasi menjadi penyebab utama kematian pada budidaya nila 42,43,44 . Di Indonesia, penyakit pada ikan nila yang disebabkan oleh S. agalactiae ada beberapa jenis serotipe.…”

PENGEMBANGAN METODE ELISA UNTUK MENDETEKSI RESPON IMUN SPESIFIK PADA IKAN NILA (Oreochromis niloticus) YANG DIVAKSINASI TERHADAP Aeromonas hydrophila DAN Streptococcus agalactiae

“…Bakteri A. hydrophila tersebar luas di perairan juga merupakan mikroflora normal pada ikan, namun apabila jumlahnya di perairan melebihi 10 4 sel per Pengembangan metode ELISA untuk mendeteksi respon imun spesifik ..... (Tuti Sumiati) mililiter maka dapat menjadi patogen (Irianto, 2003) dapat mengakibatkan kematian 10%-70% (Ibrahem et al, 2008). Sedangkan Lusiastuti et al (2009) melaporkan bahwa kasus Streptococcosis di Waduk Cirata, Jawa Barat disebabkan oleh S. agalactiae, bakteri ini dapat mengakibatkan kematian yang tinggi sekitar 60%-70% (Siti-Zahrah et al, 2008;Toranzo et al, 2009). Keberadaan infeksi gabungan (ko-infeksi) penyakit ini pada ikan nila terjadi di KJA Waduk Cirata sebesar 20% dari populasi di karamba.…”