Streptococcus australis and Ralstonia pickettii as Major Microbiota in Mesotheliomas

Higuchi, Rumi; Goto, Taichiro; Hirotsu, Yosuke; Otake, Sotaro; Oyama, Toshio; Amemiya, Kenji; Mochizuki, Hitoshi; Omata, Masao

doi:10.3390/jpm11040297

Cited by 17 publications

(10 citation statements)

References 40 publications

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“…However this study did not involve with the microbiome of BALF, as well as the relationship of microbiome between BALF and tumor tissues. In our study, we found reduced genera including Ralstonia , Lactobacillus , unidentified-Chloroplast , and Pseudomonas in the GGO group, Among them, Ralstonia pickettii was found to be a mesothelioma specific microbiota involved in tumor progression ( Higuchi et al, 2021 ), and Lactobacillus induce anticancer effect by promoting cancer cell apoptosis and preventing oxidative stress, which is common in probiotics ( Badgeley et al, 2021 ), the effect on GGO can mechanically be interpreted by carcinogenesis due to the decreased genera. Interestingly, the most dominant phylum Proteobacteria is also significantly reduced.…”

Section: Discussionmentioning

confidence: 63%

The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity

Tang

Zhuang

et al. 2022

Front. Bioeng. Biotechnol.

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Recent studies have confirmed the existence of microbiota in the lungs. The relationship between lung ground-glass opacity (GGO) and microbiota in the lung microenvironment is not clear. In this study, we investigated the microbial composition and diversity in bronchoalveolar lavage fluid (BALF) of diseased lung segments and paired contralateral healthy lung segments from 11 GGO patients. Furthermore, lung GGO and paired normal tissues of 26 GGO patients were explored whether there are microbial characteristics related to GGO. Compared with the control group, the community richness of GGO tissue and BALF of GGO lung segment (α-diversity) and overall microbiome difference (β-diversity) had no significant difference. The microbiome composition of BALF of GGO segments is distinct from that of paired healthy lung segments [genus (Rothia), order (Lachnospiraceae), family (Lachnospiraceae), genus (Lachnospiraceae_NK4A136_group, Faecalibacterium), and species (Faecalibacterium prausnitzii, Bacteroides uniforms)]. GGO tissue and adjacent lung tissue had more significant differences at the levels of class, order, family, genus, and species level, and most of them are enriched in normal lung tissue. The area under the curve (AUC) using 10 genera-based biomarkers to predict GGO was 91.05% (95% CI: 81.93–100%). In conclusion, this study demonstrates there are significant differences in the lower respiratory tract and lung microbiome between GGO and the non-malignant control group through the BALF and lung tissues. Furthermore, some potential bacterial biomarkers showed good performance to predict GGO.

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Section: Discussionmentioning

confidence: 63%

The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity

Tang

Zhuang

et al. 2022

Front. Bioeng. Biotechnol.

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“…However, the involvement of this bacterium in the carcinogenesis of any organ has not been thoroughly studied. In the study by Higuchi et al [ 55 ], Streptococcus australis and R. pickettii were identified in almost all mesothelioma patients, but it is unclear whether the bacterial profiles with high levels of bacterial composition and abundance are causally associated with oncogenesis. Future studies with larger sample sizes are needed to determine their potential association with tumorigenesis and to explore the underlying mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Species-Level Characterization of the Microbiome in Breast Tissues with Different Malignancy and Hormone-Receptor Statuses Using Nanopore Sequencing

Luo

Shi

et al. 2023

JPM

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Unambiguous evidence indicates that microbes are closely linked to various human diseases, including cancer. Most prior work investigating the microbiome of breast tissue describes an association between compositional differences of microbial species in benign and malignant tissues, but few studies have examined the relative abundance of microbial communities within human breast tissue at the species level. In this work, a total of 44 breast tissue samples including benign and malignant tissues with adjacent normal breast tissue pairs were collected, and Oxford Nanopore long-read sequencing was employed to assess breast tissue microbial signatures. Nearly 900 bacterial species were detected from the four dominant phyla: Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. The bacteria with the highest abundance in all breast tissues was Ralstonia pickettii, and its relative abundance increased with decreasing malignancy. We further examined the breast-tissue microbiome composition with different hormone-receptor statuses, and the relative abundance of the genus Pseudomonas increased most significantly in breast tissues. Our study provides a rationale for exploring microbiomes associated with breast carcinogenesis and cancer development. Further large-cohort investigation of the breast microbiome is necessary to characterize a microbial risk signature and develop potential microbial-based prevention therapies.

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“…Plasma from the blood and the supernatant of the BAL fluid was centrifuged at 20,000× g for 10 min and the resultant supernatant was subsequently transferred to sterile tubes and stored at −80 °C before DNA extraction . To obtain DNA from tumors, the formalin-fixed, paraffin-embedded (FFPE) tissue sections were first stained with hematoxylin and eosin, and the tumor lesions were microdissected using the ArcturusXT laser-capture microdissection system for the following DNA extraction as also previously described [ 7 , 10 , 11 , 12 ].…”

Section: Methodsmentioning

confidence: 99%

The Diagnostic Utility of Cell-Free DNA from Ex Vivo Bronchoalveolar Lavage Fluid in Lung Cancer

Otake

Goto

Higuchi

et al. 2022

Cancers

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Although bronchoscopy is generally performed to diagnose lung cancer, its diagnostic yield remains unsatisfactory. Assuming that lung cancer cells release cell-free DNA into the epithelial lining fluid, we hypothesized that lung cancer could be diagnosed by analyzing gene mutations in cell-free DNA in this fluid. This study included 32 patients with lung cancer who underwent surgery at our hospital. Bronchoalveolar lavage (BAL) was performed on the resected lung samples (ex vivo BAL model) after lobectomy. Each DNA sample (i.e., BAL fluid, primary lesion, and plasma) underwent deep targeted sequencing. Gene mutation analyses in the BAL fluid samples identified mutations identical to those in the primary lesions in 30 (93.8%) of 32 patients. In contrast, the microscopic cytology of the same BAL fluid samples yielded a diagnosis of lung cancer in only one of 32 patients, and the analysis of plasma samples revealed gene mutations identical to those in the primary lesions in only one of 32 patients. In conclusion, cell-free DNA released from lung cancer cells exists more abundantly in the airway than in the blood. The collection and analysis of the BAL fluid containing cell-free DNA derived from lung cancer can thus allow lung cancer diagnosis and the screening of driver mutations.

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Streptococcus australis and Ralstonia pickettii as Major Microbiota in Mesotheliomas

Cited by 17 publications

References 40 publications

The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity

The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity

Species-Level Characterization of the Microbiome in Breast Tissues with Different Malignancy and Hormone-Receptor Statuses Using Nanopore Sequencing

The Diagnostic Utility of Cell-Free DNA from Ex Vivo Bronchoalveolar Lavage Fluid in Lung Cancer

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