Streptococcus intermedius, Streptococcus constellatus, and Streptococcus anginosus (the Streptococcus milleri group): association with different body sites and clinical infections

Whiley, Robert A.; Beighton, David; Winstanley, T. G.; Fraser, H Y; Hardie, J. M.

doi:10.1128/jcm.30.1.243-244.1992

Cited by 357 publications

(201 citation statements)

References 13 publications

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“…From the available data, it cannot be determined whether this species played a causal role in the subject's clinical disease or whether the species survived the series of treatments that some of the subjects had hud prior to entry into the study. S. eonstelhitus has been implicated in other oral and extra-oral infections (Shinzalo&Saito 1994, Whiley et al 1992, Levin et al 1982. It is worth noting that the taxonomic position of S. eoustellatus has been investigated for some time because of its similarity to strains of S. intermedius and S. anginosus.…”

Section: Discussionmentioning

confidence: 99%

Clinical and microbiological features of refractory periodontitis subjects

Colombo

Haffajee

Dewhirst

et al. 1998

J Clinic Periodontology

139

161

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The purpose of this investigation was to compare the clinical parameters and the site prevalence and levels of 40 subgingival species in successfully treated and refractory periodontitis subjects. 94 subjects received scaling and root planing and if needed, periodontal surgery and systemically administered tetracycline. 28 refractory subjects showed mean full mouth attachment loss and/or > 3 sites showing attachment loss > 2.5 mm within 1 year post-therapy. 66 successfully treated subjects showed mean attachment level gain and no sites with attachment loss > 2.5 mm. Baseline subgingival plaque samples were taken from the mesial aspect of each tooth and the presence and levels of 40 subgingival taxa were determined using whole genomic DNA probes and checkerboard DNA-DNA hybridization. The mean levels and % of sites colonized by each species (prevalence) was computed for each subject and differences between groups sought using the Mann-Whitney test. Most of the 40 species tested, including Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola and Bacteroides forsythus, were equally or less prevalent in the refractory group. Prevotella nigrescens was significantly more prevalent in successfully treated subjects, while refractory subjects harbored a larger proportion of Streptococcus species, particularly Streptococcus constellatus. The odds of a subject being refractory was 8.6 (p < 0.001) if S. constellatus constituted > or = 3.5% of the total DNA probe count. Since few microbiological differences existed between treatment outcome groups using DNA probes to known species, the predominant cultivable microbiota of 33 subgingival samples from 14 refractory subjects was examined. 85% of the 1649 isolates were identified using probes to 69 recognized subgingival species. The remaining unidentified strains were classified by analyzing 16S rRNA gene sequences. Many sequenced isolates were of taxa not considered a common part of the oral microbiota such as Acinetobacter baumanni, Gemella haemolysans, Enterococcus faecalis, Staphylococcus warneri, Pseudomonas aeruginosa and novel species in the genera Bartonella, Ralstonia, Neisseria, Eubacterium, Rothia, Gordona, Gemella, Corynebacterium, Leptotrichia, and Actinomyces. Refractory subjects constituted a heterogeneous group based on their subgingival microbiota. As a group, they did not harbor more of the "classic" periodontopathogens, although elevated proportions of S. constellatus were found.

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Section: Discussionmentioning

confidence: 99%

Clinical and microbiological features of refractory periodontitis subjects

Colombo

Haffajee

Dewhirst

et al. 1998

J Clinic Periodontology

139

161

View full text Add to dashboard Cite

show abstract

“…Furthermore, among the group, the pathogenicity of each species seems to be different. Whiley et al [5] reported that Streptococcus intermedius was associated with abscesses of the brain and liver, while Streptococcus anginosus and Streptococcus constellatus (S. constellatus subsp. constellatus) were isolated from a wider range of sites and infections.…”

Section: Introductionmentioning

confidence: 99%

Identification of the anginosus group within the genusStreptococcususing polymerase chain reaction

Takao

Nagamune

Maeda

2004

FEMS Microbiology Letters

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The aim of this study was to establish an identification method for the anginosus group within the genus Streptococcus by polymerase chain reaction (PCR). Using a primer pair based on the group-specific sequences of penicillin-binding protein 2B (pbp2b) gene, a 275-bp fragment was amplified from each species in the group but no size-matched products were obtained in other streptococci. Further identification in the species or subspecies level was possible by a multiplex PCR with primers for the 16S ribosomal RNA gene of Streptococcus anginosus, the hyaluronate lyase genes both of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus, and the intermedilysin (ily) gene of S. intermedius. In the case ofStreptococcus constellatus subsp. pharyngis, the amplified fragment from the S. intermedius-type hyaluronate lyase gene was obtained, while that from the ily gene was not. These results also indicate that two different hyaluronate lyase genes are distributed among the anginosus group.

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“…It is often associated with pyogenic infections, including endocarditis. [7][8][9] S. anginosus DNA has also been found in head and neck squamous cell carcinomas 10) ; it was found much less frequently in non-cancerous tissues of the esophagus and was absent from the colon, lung, bladder, renal, and cervical cancer tissues. 6) Therefore, it was suggested that S. anginosus DNA is associated with cancers in the upper digestive tract, although the involvement of S. anginosus infection in the carcinogenic process has not been clarified.…”

mentioning

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Different frequencies of Streptococcus anginosus infection in oral cancer and esophageal cancer

Morita¹,

Narikiyo

Yano³

et al. 2003

Cancer Science

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Multiple cancers frequently occur in the upper aerodigestive tract. The high incidence rate of multiple carcinomas in this region is often explained in terms of involvement of the same underlying risk factors. It has been reported that the oral bacterium Streptococcus anginosus (S. anginosus) is associated with esophageal, gastric, and pharyngeal cancer tissues. In this study, a highly specific quantification method for S. anginosus DNA using real-time PCR was established. We employed this assay to determine whether S. anginosus is also associated with oral cancer tissues. This precise quantification method revealed different degrees of infection with S. anginosus in esophageal cancer and oral cancer. We assayed 10 ng of genomic DNA from cancer tissues, and found that eight of 18 samples (44%) from the esophagus contained a detectable level ( > > > >10 fg) of S. anginosus DNA, whereas this was the case for only five of 38 samples (13%) of oral cancer. The quantity of S. anginosus DNA in the esophageal cancer tissues was significantly higher than in oral cancer. The maximum amount of S. anginosus DNA was approximately ten times higher in esophageal than in oral cancer tissues. In addition, none of the five different oral cancer sites (floor of the mouth, mandibular gingival, maxillary gingival, buccal mucosal, and tongue) showed significant signs of S. anginosus infection. On the other hand, most non-cancerous tissues of the esophagus and tongue showed an undetectable level of S. anginosus. These results suggest that S. anginosus is associated with esophageal cancer, but is not closely related with oral cancer. (Cancer Sci 2003; 94: 492-496) acterial and viral infections are important factors in cancer development.1) It has been reported that Helicobacter pylori (H. pylori) is associated with gastritis, gastric atrophy, and gastric cancer.2-4) The presence of microorganisms in several kinds of human cancers was recently investigated, and Streptococcus anginosus (S. anginosus) DNA fragments were frequently found in DNA samples from esophageal cancer tissues, gastric cancer tissues, and dysplasia of the esophagus.5, 6) Viable S. anginosus was also recovered from esophageal cancer tissues (unpublished data). These results suggest that S. anginosus infection occurs at an early stage of esophageal cancer and is related to esophageal and gastric carcinogenesis. S. anginosus is classified as an oral bacterium and can be isolated from several parts of the body such as the oral cavity, gastrointestinal tract, and genitourinary tract. It is often associated with pyogenic infections, including endocarditis. 7-9) S. anginosus DNA has also been found in head and neck squamous cell carcinomas 10) ; it was found much less frequently in non-cancerous tissues of the esophagus and was absent from the colon, lung, bladder, renal, and cervical cancer tissues. 6) Therefore, it was suggested that S. anginosus DNA is associated with cancers in the upper digestive tract, although the involvement of S. anginosus infection in the carc...

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Streptococcus intermedius, Streptococcus constellatus, and Streptococcus anginosus (the Streptococcus milleri group): association with different body sites and clinical infections

Cited by 357 publications

References 13 publications

Clinical and microbiological features of refractory periodontitis subjects

Clinical and microbiological features of refractory periodontitis subjects

Identification of the anginosus group within the genusStreptococcususing polymerase chain reaction

Different frequencies of Streptococcus anginosus infection in oral cancer and esophageal cancer

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