Streptococcus mutans attachment on a cast titanium surface

Abstract

This study examined by means of scanning electron microscopy (SEM), the attachment of Streptococcus mutans and the corrosion of cast commercially pure titanium, used in dental dentures. The sample discs were cast in commercially pure titanium using the vacuum-pressure machine (Rematitan System). The surfaces of each metal were ground and polished with sandpaper (#300-4000) and alumina paste (0.3 μm). The roughness of the surface (Ra) was measured using the Surfcorder rugosimeter SE 1700. Four coupons were inserted separately into Falcon tubes contained Mueller Hinton broth inoculated with S. mutans ATCC 25175 (10 9 cuf) and incubated at 37 °C. The culture medium was changed every three days during a 365-day period, after which the falcons were prepared for observations by SEM. The mean Ra value of CP Ti was 0.1527 μm. After S. mutans biofilm removal, pits of corrosion were observed. Despite the low roughness, S. mutans attachment and biofilm formation was observed, which induced a surface corrosion of the cast pure titanium.

Effects of Various Polishing Techniques on the Surface Characteristics of the Ti-6Al-4V Alloy and on Bacterial Adhesion

“…The colony forming unit (CFU) [12] was used to measure the number of colonies to estimate the number of accumulated bacteria. The variation factors for the inhibition or adhesion characteristics of bacteria are mainly the surface characteristics of the implant, including surface roughness [13][14][15][16], contact angle [17,18], and oxygen content [19][20][21].…”

Surface Coating of Gypsum‐Based Molds for Maxillofacial Prosthetic Silicone Elastomeric Material: Evaluating Different Microbial Adhesion

“…Sterile BHI broth and SDB were renewed at an interval of 3 days for 30 days, and OD was evaluated before media were replaced. Afterward, the specimens were removed from the culture medium …”

Bacterial interactions with biomaterials metallic surfaces

“…Aykent et al 21 observed a positive correlation between surface roughness and S. mutans adhesion. Several studies shown the oral bacterial adherence on rough surfaces of dental implant biomaterials 22,23 . The concern with the implant surface that comes in contact with the host tissue is with the osseointegration, and nevertheless there are no studies on bacterial adhesion and his effects on titanium surface modified by Nd:YAG laser process.…”