Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling

Wang, Ling; Zhang, Xuemei; Wu, Guangying; Qi, Yuhong; Zhang, Jinghui; Yang, Jing; Wang, Hong; Xu, Wenchun

doi:10.1007/s12275-020-9538-0

Cited by 9 publications

(8 citation statements)

References 35 publications

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“…The PI3K-Akt and MAPK pathways are interconnected with each other [ 36 , 37 ]. The activation of the PI3K-Akt signaling pathway and the ERK-related intracellular MAPK signaling pathway was correlated with endometriosis [ 36 ], and both were shown to be involved in the immunity [ 38 ]. In addition, our research further identified the involvement of IRGs in the regulation of the PI3K-Akt signaling pathway and MAPK signaling pathway in endometriosis.…”

Section: Discussionmentioning

confidence: 99%

Identification and Analysis of Potential Immune-Related Biomarkers in Endometriosis

et al. 2023

Journal of Immunology Research

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Background. Endometriosis is an inflammatory gynecological disease leading to deep pelvic pain, dyspareunia, and infertility. The pathophysiology of endometriosis is complex and depends on a variety of biological processes and pathways. Therefore, there is an urgent need to identify reliable biomarkers for early detection and accurate diagnosis to predict clinical outcomes and aid in the early intervention of endometriosis. We screened transcription factor- (TF-) immune-related gene (IRG) regulatory networks as potential biomarkers to reveal new molecular subgroups for the early diagnosis of endometriosis. Methods. To explore potential therapeutic targets for endometriosis, the Gene Expression Omnibus (GEO), Immunology Database and Analysis Portal (ImmPort), and TF databases were used to obtain data related to the recognition of differentially expressed genes (DEGs), differentially expressed IRGs (DEIRGs), and differentially expressed TFs (DETFs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on the DETFs and DEIRGs. Then, DETFs and DEIRGs were further validated in the external datasets of GSE51981 and GSE1230103. Then, we used quantitative real-time polymerase chain reaction (qRT-PCR) to verify the hub genes. Simultaneously, the Pearson correlation analysis and protein-protein interaction (PPI) analyses were used to indicate the potential mechanisms of TF-IRGs at the molecular level and obtain hub IRGs. Finally, the receiver operating characteristic (ROC) curve analysis was used to assess the diagnostic value of the hub IRGs. Results. We screened a total of 94 DETFs and 121 DEIRGs in endometriosis. Most downregulated DETFs showed decreased expression in the endometria of moderate/severe endometriosis patients. The top-ranked upregulated DEIRGs were upregulated in the endometra of infertile women. Functional analysis showed that DETFs and DEIRGs may be involved in the biological behaviors and pathways of endometriosis. The TF-IRG PPI network was successfully constructed. Compared with the control group, high C3, VCAM1, ITGB2, and C3AR1 expression had statistical significance in endometriosis among the hub DEIRGs. They also showed higher sensitivity and specificity by ROC analysis for the diagnosis of endometriosis. Finally, compared with controls, C3 and VCAM1 were highly expressed in endometriosis tissue samples. In addition, they also showed high specificity and sensitivity for diagnosing endometriosis. Conclusion. Overall, we discovered the TF-IRG regulatory network and analyzed 4 hub IRGs that were closely related to endometriosis, which contributes to the diagnosis of endometriosis. Additionally, we verified that DETFs or DEIRGs were associated with the clinicopathological features of endometriosis, and external datasets also confirmed the hub IRGs. Finally, C3 and VCAM1 were highly expressed in endometriosis tissue samples compared with controls and may be potential biomarkers of endometriosis, which are helpful for the early diagnosis of endometriosis.

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Section: Discussionmentioning

confidence: 99%

Identification and Analysis of Potential Immune-Related Biomarkers in Endometriosis

et al. 2023

Journal of Immunology Research

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“…Researchers have discovered that as a result of exposure to Veillonella , Prevotella , and Streptococcus bacteria , epithelial cells are transformed both in vitro and in vivo by activating ERK and PI3K (Figure 3B ). 131 , 252 , 253 …”

Section: Mechanisms By Which Human Carcinogenic Microbiomes Promote C...mentioning

confidence: 99%

Human microbiomes in cancer development and therapy

Xia

Liu

et al. 2023

MedComm

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Colonies formed by bacteria, archaea, fungi, and viral groups and their genomes, metabolites, and expressed proteins constitute complex human microbiomes. An increasing evidences showed that carcinogenesis and disease progression were link to microbiomes. Different organ sources, their microbial species, and their metabolites are different; the mechanisms of carcinogenic or procancerous are also different. Here, we summarize how microbiomes contribute to carcinogenesis and disease progression in cancers of the skin, mouth, esophagus, lung, gastrointestinal, genital, blood, and lymph malignancy. We also insight into the molecular mechanisms of triggering, promoting, or inhibiting carcinogenesis and disease progress induced by microbiomes or/and their secretions of bioactive metabolites. And then, the strategies of application of microorganisms in cancer treatment were discussed in detail. However, the mechanisms by which human microbiomes function are still poorly understood. The bidirectional interactions between microbiotas and endocrine systems need to be clarified. Probiotics and prebiotics are believed to benefit human health via a variety of mechanisms, in particular, in tumor inhibition. It is largely unknown how microbial agents cause cancer or how cancer progresses. We expect this review may open new perspectives on possible therapeutic approaches of patients with cancer.

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“…Previous studies have shown that it was involved in various cellular processes such as proliferation, differentiation, and migration of intestinal immune cells and promoted innate immune responses by activating phagocytic immune responses (78)(79)(80). Meanwhile, MAPK signaling pathway has also been found to regulate the production of pro-inflammatory factors such as IL-1b, TNF-a, and TFN-g to promote inflammatory responses (79,81,82). In this study, MAPK signaling pathway was significantly enriched after Cd and Cu co-exposure, indicating that co-exposure might induce the proliferation and differentiation of S. esculenta immune cells and promoted inflammatory and innate immune response processes.…”

Section: Mapk Signaling Pathway Analysismentioning

confidence: 99%

Exploration of immune response mechanisms in cadmium and copper co-exposed juvenile golden cuttlefish (Sepia esculenta) based on transcriptome profiling

et al. 2022

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Sepia esculenta is a popular economic cephalopod with high yield, delicious meat, and rich nutrition. With the rapid development of heavy industry and medical industry, a large amount of waste has been released into the ocean recklessly in recent years, inducing a significant increase in the content of heavy metals, especially cadmium (Cd) and copper (Cu), in the ocean. This phenomenon significantly affects the growth and development of S. esculenta, causing a serious blow to its artificial breeding. In this study, transcriptome analysis is used to initially explore immune response mechanisms of Cd and Cu co-exposed juvenile S. esculenta. The results show that 1,088 differentially expressed genes (DEGs) are identified. And DEGs functional enrichment analysis results suggests that co-exposure may promote inflammatory and innate immune responses in juvenile S. esculenta. Fifteen key genes that might regulate the immunity of S. esculenta are identified using protein-protein interaction (PPI) network and KEGG enrichment analyses, of which the three genes with the highest number of interactions or involve in more KEGG pathways are identified as hub genes that might significantly affect the immune response processes. Comprehensive analysis of PPI network and KEGG signaling pathway is used for the first time to explore co-exposed S. esculenta juvenile immune response processes. Our results preliminarily reveal immune response mechanisms of cephalopods exposed to heavy metals and provide a valuable resource for further understanding of mollusk immunity.

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Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling

Cited by 9 publications

References 35 publications

Identification and Analysis of Potential Immune-Related Biomarkers in Endometriosis

Identification and Analysis of Potential Immune-Related Biomarkers in Endometriosis

Human microbiomes in cancer development and therapy

Exploration of immune response mechanisms in cadmium and copper co-exposed juvenile golden cuttlefish (Sepia esculenta) based on transcriptome profiling

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