Streptomyces ansochromogenes Tur-10 produces a substance with antifungal bioactivity

Abstract

ABSTRACT. The increased incidence of fungal infections and the development of drug resistance have led to the search for microorganisms capable of producing bioactive metabolites with antifungal activity. Among these microorganisms, Streptomyces spp are distinguished mainly owing to their potential to secrete bioactive molecules. The aim of this study was to evaluate the production of secondary metabolites by Streptomyces sp TUR-10 against 12 fungal clinical isolates (yeast and filamentous fungi). In the preliminary screening, Streptomyces sp TUR-10 showed activity against 75% of the clinical isolates, and was selected for fermentation. In this assay, we tested three different media (MPE, M1, and ISP-4) for 96 h at pH 7.0 and 30°C for the production of bioactive metabolites. Increased production of bioactive compounds was observed when using the MPE medium for 48 h, with good activity against Candida pelliculosa. The minimum inhibitory concentration showed significant antifungal activity values ranging from 15.6 to 250 µg/mL. The actinobacterium was characterized by 16S rRNA analysis N.M. Vasconcelos et al. 5436©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 5435-5444 (2015) and the pattern suggested that the isolate studied belonged to the species Streptomyces ansochromogenes. The biotechnological potential of this strain was also demonstrated by the detection of the nrps and pks genes. These results indicate the production of secondary metabolites of biotechnological interest by actinobacteria from the rhizosphere, suggesting great potential for further research.

L‐asparaginase isolated fromStreptomyces ansochromogenespromotes Th1 profile and activatesCD8+T cells in humanPBMC: anin vitroinvestigation

“…; Vasconcelos et al . ). Moreover, bioactive compounds produced by members of the genus Streptomyces have been researched and L‐asparaginase enzyme production has also been widely explored (Meena et al .…”

“…Streptomyces is the most important genus of this group because of its relevant capacity and versatility to produce metabolites such as antibiotics, herbicides, insecticides, antifungals, antitumour or immunosuppressant drugs, and anthelmintic agents (Behal 2000;Inbar et al 2005;Hopwood 2007; Barka et al 2016;Silva-Lacerda et al 2016). Streptomyces ansochromogenes is a species that can be isolated from the soil environment and is related to producers of antibiotics like Nikkomycin and Tylosin (Liao et al, 2010;Lu et al 2015;Vasconcelos et al 2015). Moreover, bioactive compounds produced by members of the genus Streptomyces have been researched and L-asparaginase enzyme production has also been widely explored (Meena et al 2015).…”

Antimicrobial potential of actinobacteria isolated from the rhizosphere of the Caatinga biome plant Caesalpinia pyramidalis Tul.

“…After 120 h, an agar block diffusion assay was performed using test microorganisms strains from the Microorganism Culture Collection of Departamento de Antibióticos, Universidade Federal de Pernambuco (UFPEDA). Species included for testing were: Fusarium moniliforme UFPEDA-2456; Malassezia furfur UFPEDA-1320; Candida albicans UFPEDA-1007; Bacillus subtilis UFPEDA-86; Staphylococcus aureus UFPEDA-02; methicillin-resistant S. aureus (MRSA) UFPEDA-700; Escherichia coli UFPEDA-224; Klebsiella pneumoniae UFPEDA-416; and Pseudomonas aeruginosa UFPEDA-396 (Vasconcelos et al, 2015).…”