2022
DOI: 10.1128/aem.01881-21
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Streptomyces coelicolor Vesicles: Many Molecules To Be Delivered

Abstract: Streptomycetes are widely distributed in nature and characterized by a complex life cycle that involves morphological differentiation. They are very relevant in industry because they produce about half of all clinically used antibiotics, as well as other important pharmaceutical products of natural origin. Streptomyces coelicolor is a model organism for the study of bacterial differentiation and bioactive molecule production. S. coelicolor produces extracellular … Show more

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Cited by 28 publications
(23 citation statements)
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“…Two main populations of MVs, namely F3 and F4 MVs, were isolated and purified from liquid cultures of S. coelicolor according to the protocol reported in Materials and Methods. These two MV populations were previously characterized: besides having different diameters (average sizes were about 100 nm and 200 nm in F3 and F4, respectively), they differed also for the composition of their protein and metabolite cargos 4 . All six gradient fraction were directly loaded on a 1% (w/v) agarose gel and this electrophoresis analysis revealed the presence of three main bands: two smaller bands corresponding to approximately 900 bp and 500 bp, respectively, and a higher molecular weight band of approximately 19 kb (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…Two main populations of MVs, namely F3 and F4 MVs, were isolated and purified from liquid cultures of S. coelicolor according to the protocol reported in Materials and Methods. These two MV populations were previously characterized: besides having different diameters (average sizes were about 100 nm and 200 nm in F3 and F4, respectively), they differed also for the composition of their protein and metabolite cargos 4 . All six gradient fraction were directly loaded on a 1% (w/v) agarose gel and this electrophoresis analysis revealed the presence of three main bands: two smaller bands corresponding to approximately 900 bp and 500 bp, respectively, and a higher molecular weight band of approximately 19 kb (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…1 ). According to previous works, F3 and F4 were chosen for further investigations as they are the most MV-enriched gradient fractions 4 , 37 . Moreover, presence of MVs in F3 and F4 was confirmed through Dynamic Light Scattering (DLS) analysis (Supplementary Fig.…”
Section: Resultsmentioning
confidence: 99%
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