Streptomyces griseocarneus R132 expresses antimicrobial genes and produces metabolites that modulate Galleria mellonella immune system

Siqueira, Kátia Aparecida de; Liotti, Rhavena Graziela; Sousa, Janaína Rosa de; Vendruscullo, Suzana Junges; Souza, Gilza Barcelos de; Vasconcelos, Leonardo Gomes de; Januário, Ana Helena; Mendes, Tiago; Soares, Marcos Antônio

doi:10.1007/s13205-021-02942-1

Cited by 3 publications

(1 citation statement)

References 97 publications

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“…As mentioned earlier, due to their partly lipophilic character, the ability of these compounds to acidify the cytoplasm by passing through the cell membrane by passive diffusion depends on the number of hydroxyls (-OH), methoxy (-OCH3), carboxyl (−COOH), functional groups and saturation of alkyl side chain [27,43]. As previously mentioned, even though phenolic acids and their derivatives are widely isolated from the plant kingdom, recent investigations indicated their presence in other sources, including marine-derived microorganisms, plant-derived endophytic fungus, marine organisms, and bacterium species [34][35][36][37]. Several studies have been conducted to emphasize the importance of phenolic compounds in the ability to develop resistance in multidrug-resistant bacteria.…”

Section: Simple Phenols and Phenolic Acidsmentioning

confidence: 99%

Preventing Microbial Infections with Natural Phenolic Compounds

Ecevit

Barros

Silva

et al. 2022

Future Pharmacology

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The struggle between humans and pathogens has taken and is continuing to take countless lives every year. As the misusage of conventional antibiotics increases, the complexity associated with the resistance mechanisms of pathogens has been evolving into gradually more clever mechanisms, diminishing the effectiveness of antibiotics. Hence, there is a growing interest in discovering novel and reliable therapeutics able to struggle with the infection, circumvent the resistance and defend the natural microbiome. In this regard, nature-derived phenolic compounds are gaining considerable attention due to their potential safety and therapeutic effect. Phenolic compounds comprise numerous and widely distributed groups with different biological activities attributed mainly to their structure. Investigations have revealed that phenolic compounds from natural sources exhibit potent antimicrobial activity against various clinically relevant pathogens associated with microbial infection and sensitize multi-drug resistance strains to bactericidal or bacteriostatic antibiotics. This review outlines the current knowledge about the antimicrobial activity of phenolic compounds from various natural sources, with a particular focus on the structure-activity relationship and mechanisms of actions of each class of natural phenolic compounds, including simple phenols, phenolic acids, coumarin, flavonoids, tannins, stilbenes, lignans, quinones, and curcuminoids.

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Section: Simple Phenols and Phenolic Acidsmentioning

confidence: 99%

Preventing Microbial Infections with Natural Phenolic Compounds

Ecevit

Barros

Silva

et al. 2022

Future Pharmacology

View full text Add to dashboard Cite

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In vitro antimicrobial and antioxidant activities of bioactive compounds extracted from Streptomyces africanus strain E2 isolated from Moroccan soil

Rammali,

Kamal,

El Aalaoui

et al. 2024

Sci Rep

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This study aimed to isolate Streptomyces sp. from Moroccan terrestrial ecosystems and identify bioactive compounds through GC–MS analysis. Antimicrobial activity was assessed against various pathogenic microorganisms including Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and Candida albicans ATCC 60193, and multi-drug resistant strains comprising Listeria monocytogenes , Klebsiella pneumoniae 19K 929, Proteus sp. 19K1313, Klebsiella pneumoniae 20B1572, Proteus vulgaris 16C1737, and Klebsiella pneumoniae 20B1572. Based on the results of the gene sequencing of gene 16S rRNA and phylogenetic analysis, the E2 isolate belongs to the genus Streptomyces with the highest degree of resemblance (97.51%) to the Streptomyces africanus strain NBRC 101005 (NR_112600.1). The isolate exhibited broad-spectrum antibacterial activity, with maximum efficacy against Klebsiella pneumoniae 20B1572 indicated by an inhibition zone diameter of 22.5 ± 0.71mm and a minimum inhibitory concentration (MIC) of 0.0625 mg/mL. The in vitro antioxidant potential of E2 strain was determined through screening of its ethyl acetate extract against sets of antioxidant assays. The results were indicative of E2 strain displaying strong antioxidant activity against ABTS, DPPH free radicals, and FRAP. Furthermore, there was a high significant correlation (p < 0.0001) between the total phenolic and flavonoid content and antioxidant activities. The GC–MS analysis of the extract identified six volatile compounds, with Eugenol (96%) and Maltol (93%) being the most prominent. Additionally, the HPLC–UV/vis analysis revealed six phenolic compounds: gallic acid, chlorogenic acid, vanillic acid, trans-ferulic acid, ellagic acid, and cinnamic acid. Overall, the study highlights Streptomyces sp. strain E2 as a potential source of potent antimicrobial and antioxidant metabolites, offering promise in addressing antibiotic resistance and oxidative stress-related conditions.

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Galleria Mellonella Larvae as an In vitro Model for Testing Microbial Pathogenicity

Gniazdo,

Sasal,

Omelaniuk

et al. 2023

Advancements of Microbiology

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The larvae of the insect Galleria mellonella have gained wide acceptance as an in vivo model for the study of virulence in Gram-negative and Gram-positive bacteria and fungi. Importantly, G. mellonella offers an alternative, low-cost, and ethically acceptable method of assessing pathogens. These insects have a number of advantages, including ease of breeding, a rapid developmental cycle, low maintenance costs, and ease of experimental manipulation. Most importantly, G. mellonella larvae have a complete immune system and a nonspecific immune response similar to that of humans, making them a valuable model for studying pathogen-host interactions. Furthermore, using this model, it is possible to test the efficacy of various chemical compounds or natural substances that show antimicrobial activity. In this literature review, we provide a comprehensive overview of recent studies of the use of G. mellonella in virulence analyses and as a platform for testing the efficacy of new drugs.

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Streptomyces griseocarneus R132 expresses antimicrobial genes and produces metabolites that modulate Galleria mellonella immune system

Cited by 3 publications

References 97 publications

Preventing Microbial Infections with Natural Phenolic Compounds

Preventing Microbial Infections with Natural Phenolic Compounds

In vitro antimicrobial and antioxidant activities of bioactive compounds extracted from Streptomyces africanus strain E2 isolated from Moroccan soil

Galleria Mellonella Larvae as an In vitro Model for Testing Microbial Pathogenicity

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