Streptomyces manganisoli sp. nov., a novel actinomycete isolated from manganese-contaminated soil

Mo, Ping; Zhao, Jiarong; Li, Kaiqin; Tang, Xinke; Gao, Jian

doi:10.1099/ijsem.0.002762

“…In Table 3 , we depict novel heavy metal-resistant species which were isolated from metal-contaminated areas. The literature review ( Table S1 : List of strains from heavy metal-contaminated areas) suggests that many Streptomyces species with unique characteristics were isolated from soils of metal-contaminated areas [ 32 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ]. For example, Schmidt et al isolated streptomycetes highly resistant to nickel from a polluted site at the former uranium mine [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

Cimermanova

¹

,

Pristaš

²

,

Piknová

³

2021

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Technosols are artificial soils generated by diverse human activities and frequently contain toxic substances resulting from industrial processes. Due to lack of nutrients and extreme physico-chemical properties, they represent environments with limited bacterial colonization. Bacterial populations of technosols are dominated usually by Actinobacteria, including streptomycetes, known as a tremendous source of biotechnologically important molecules. In this study, the biodiversity of streptomycete-like isolates from several technosols, mainly mine soils and wastes (landfills and sludge) in Slovakia, was investigated. The combination of basic morphological and biochemical characterisations, including heavy metal resistance determination, and molecular approaches based on 16S rRNA gene analysis were used for the identification of the bacterial strains. From nine isolates of Actinobacteria collected from different habitats, one was found to represent a new species within the Crossiella genus. Eight other isolates were assigned to the genus Streptomyces, of which at least one could represent a new bacterial species. Some isolates showed high resistance to Pb, Zn, Cu or Ni. The most tolerated metal was Pb. The results obtained in this study indicate that technosols are a prospective source of new actinomycete species resistant to heavy metals what underlines their bioremediation potential.

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“…Then, the tissue surfaces were sterilized according to a procedure described previously [10]. GGCR-6 T was isolated and purified by the methods described by Mo et al [11]. The purified isolate was maintained on Gauze's synthetic agar [12] at 4 °C and stored in a 30 % (w/v) glycerol suspension at −80 °C.…”

mentioning

confidence: 99%

Streptomyces aquilus sp. nov., a novel actinomycete isolated from a Chinese medicinal plant

Li

¹

,

Guo

²

,

Wang³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The taxonomic position of a novel actinomycete isolate, designated strain GGCR-6T, isolated from the healthy leaves of Xanthium sibiricum collected from the botanic garden of Hunan University of Science and Technology in Hunan province, PR China, was determined by a polyphasic approach. GGCR-6T grew well on ISP series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into straight spore chains consisting of cylindrical spores with smooth surfaces. The diagnostic diamino acid was ll-diaminopimelic acid. The major menaquinones were MK-9(H8), MK-9(H2), MK-9 and MK-9(H6). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphotidylinositol and phosphatidylinositol mannosides. The predominant fatty acids were C16 : 1ω9c, iso-C16 : 0 and C16 : 0. The phenotypic characteristics of GGCR-6T indicated that it represented a member of the genus Streptomyces . Phylogenetic analysis based on the 16S rRNA gene sequence indicated that GGCR-6T was most closely related to Streptomyces cyaneus NRRL B2296T and Streptomyces griseoruber NRRL B1818T. However, the digital DNA–DNA hybridization, the average nucleotide identity and the multi locus sequence analysis evolutionary distance clearly separate GGCR-6T from the phylogenetically closely related species. Furthermore, the novel isolate was distinctly differentiated from S. cyaneus NRRL B2296T and S. griseoruber NRRL B1818T by morphological, physiological and biochemical characteristics. Based on these data, strain GGCR-6T should be designated as a representative of a novel species of the genus Streptomyces , for which the name Streptomyces aquilus sp. nov. is proposed. The type strain is strain GGCR-6T (=CICC 11055T=JCM 33584T).

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“…Therefore, more attention is being focused on Streptomyces strains from unusual and unexplored environments, such as soil polluted by heavy metals [7][8][9][10][11][12], salt lakes [13], seawater [14][15][16], animals [17,18], plants [19,20] and rhizosphere soil [21,22]. During an investigation into the diversity of endophytic and rhizosphere soil actinobacteria from Broussonetia papyrifera, hundreds of strains were isolated by using different media.…”

mentioning

confidence: 99%

Streptomyces phaeolivaceus sp. nov. and Streptomyces broussonetiae sp. nov., isolated from the leaves and rhizosphere soil of Broussonetia papyrifera

Mo

¹

,

Liu

²

,

Zhao

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Two novel actinobacteria, designated strains GY16T and T44T, were isolated from the leaves and rhizosphere soil of Broussonetia papyrifera, respectively. A polyphasic approach was used for determining their taxonomic position. Results of 16S rRNA gene sequence analysis indicated that strain GY16T exhibited highest similarities to Streptomyces cinereoruber subsp. fructofermentans CGMCC 4.1593T (98.82 %), Streptomyces deccanensis KCTC 19241T (98.76 %), Streptomyces scabiei NRRL B-16523T (98.69 %), Streptomyces europaeiscabiei KACC 20186T (98.69 %) and Streptomyces rishiriensis NBRC 13407T (98.69 %), and strain T44T showed 99.2, 99.1, 99.1 and <98.7 % sequence similarities to Streptomyces filipinensis CGMCC 4.1452T, Streptomyces achromogenes subsp. achromogenes DSM 40028T, Streptomyces durhamensis DSM 40539T and other Streptomyces species, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GY16T formed an independent subclade, which indicated that strain GY16T should belong to a potential novel species; and strain T44T was closely related to S. filipinensis CGMCC 4.1452T, S. achromogenes subsp. achromogenes DSM 40028T, S. durhamensis DSM 40539T and S. yokosukanensis DSM 40224T. However, the multilocus sequence analysis evolutionary distance, average nucleotide identity and DNA–DNA hybridization values between closely related relatives were far from the species-level thresholds. In addition, phenotypic and chemotaxonomic characteristics further confirmed that strains GY16T and T44T belonged to two distinct species. Based on these results, it is concluded that the isolated strains represent novel species within the genus Streptomyces , for which the names Streptomyces phaeolivaceus sp. nov. (type strain GY16T=CICC 24807T=KCTC 49326T) and Streptomyces broussonetiae sp. nov. (type strain T44T=CICC 24819T=JCM 33918T) are proposed.

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Streptomyces manganisoli sp. nov., a novel actinomycete isolated from manganese-contaminated soil

Cited by 16 publications

References 31 publications

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

Streptomyces aquilus sp. nov., a novel actinomycete isolated from a Chinese medicinal plant

Streptomyces phaeolivaceus sp. nov. and Streptomyces broussonetiae sp. nov., isolated from the leaves and rhizosphere soil of Broussonetia papyrifera

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