Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties

Abstract

Reactive oxygen species and other radicals potentially cause oxidative damage to proteins, lipids, and DNA which may ultimately lead to various complications including mutations, carcinogenesis, neurodegeneration, cardiovascular disease, aging, and inflammatory disease. Recent reports demonstrate that Streptomyces bacteria produce metabolites with potent antioxidant activity that may be developed into therapeutic drugs to combat oxidative stress. This study shows that Streptomyces sp. MUM212 which was isolated from mangrove soil in Kuala Selangor, Malaysia, could be a potential source of antioxidants. Strain MUM212 was characterized and determined as belonging to the genus Streptomyces using 16S rRNA gene phylogenetic analysis. The MUM212 extract demonstrated significant antioxidant activity through DPPH, ABTS and superoxide radical scavenging assays and also metal-chelating activity of 22.03 ± 3.01%, 61.52 ± 3.13%, 37.47 ± 1.79%, and 41.98 ± 0.73% at 4 mg/mL, respectively. Moreover, MUM212 extract was demonstrated to inhibit lipid peroxidation up to 16.72 ± 2.64% at 4 mg/mL and restore survival of Vero cells from H2O2-induced oxidative damages. The antioxidant activities from the MUM212 extract correlated well with its total phenolic contents; and this in turn was in keeping with the gas chromatography–mass spectrometry analysis which revealed the presence of phenolic compounds that could be responsible for the antioxidant properties of the extract. Other chemical constituents detected included hydrocarbons, alcohols and cyclic dipeptides which may have contributed to the overall antioxidant capacity of MUM212 extract. As a whole, strain MUM212 seems to have potential as a promising source of novel molecules for future development of antioxidative therapeutic agents against oxidative stress-related diseases.

Genome Mining Revealed a High Biosynthetic Potential for Antifungal Streptomyces sp. S-2 Isolated from Black Soot

“…S-2, aside from antibiotic activity, might display antitumor, anti-inflammatory, or antioxidant activities, or function as insecticides. Such activities have been previously reported in the Streptomyces genus [2,10,[70][71][72]. Considering the biosynthetic potential of the S-2 strain, future research focusing on investigation of these activities is ensured.…”

Investigating the antioxidant potential of Streptomyces sp. MUSC 11 from mangrove soil in Malaysia

“…It has been suggested that understudied ecosystem hold Streptomyces species which can meet the growing demand of drug discovery and development industry [77] . Researchers who made an effort to study Streptomyces from less explored ecosystems such as mangrove forest were able to discover novel Streptomyces species and Streptomyces strains showing potent antioxidant activities [40,42,66,68,78,79] . dards for potential match.…”

“…The total phenolic content (TPC) of the methanolic extract of MUSC 11 were determined following the method of Tan et al [66] . A series of concentrations of the extract at 10 µL each were prepared and added into 96 well.…”

“…The radical scavenging activity of the methanolic extract was examined in accordance with method of Tan et al [66] . Briefly, the reagent, 2, 20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was used to generate ABTS radical ion (ABTS• + ).…”

“…The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was carried out in accordance to previous method stated elsewhere [66] . A solution of pre-made DPPH (0.016 % w/v) dissolved in ethanol (95 % v/v) was added into 96 well plates containing different concentrations of methanolic extract and left standing for 20 minutes, in dark at room temperature.…”