2017
DOI: 10.3389/fmicb.2017.00432
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Streptomyces venezuelae ISP5230 Maintains Excretion of Jadomycin upon Disruption of the MFS Transporter JadL Located within the Natural Product Biosynthetic Gene Cluster

Abstract: JadL was identified as a Major Facilitator Superfamily (MFS) transporter (T.C. 2.A.1) through sequence homology. The protein is encoded by jadL, situated within the jadomycin biosynthetic gene cluster. JadL has, therefore, been assigned a putative role in host defense by exporting its probable substrates, the jadomycins, a family of secondary metabolites produced by Streptomyces venezuelae ISP5230. Herein, we evaluate this assumption through the construction and analysis of a jadL disrupted mutant, S. venezuel… Show more

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Cited by 9 publications
(7 citation statements)
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“…Chloramphenicol is a broad-spectrum antibacterial, active against both Gram-positive and Gram-negative bacteria and inhibits protein synthesis by binding to the 50S ribosome subunit [40,62]. The jadomycins are angucycline antibiotics with a cytotoxic activity whose expression is induced via ethanol, phage or yeast co-culture shock when grown in minimal media [63]. Regulation of jadomycin biosynthesis by CSRs is complex, but the current model begins with the gamma-butyrolactone SVB1 modulating the activity of the γ-butyrolactone receptor JadR3, which activates transcription of the gene encoding the OmpR-type atypical response regulator jadR1 and represses jadR2 .…”
Section: Coordinate Regulation Of Bgcsmentioning
confidence: 99%
“…Chloramphenicol is a broad-spectrum antibacterial, active against both Gram-positive and Gram-negative bacteria and inhibits protein synthesis by binding to the 50S ribosome subunit [40,62]. The jadomycins are angucycline antibiotics with a cytotoxic activity whose expression is induced via ethanol, phage or yeast co-culture shock when grown in minimal media [63]. Regulation of jadomycin biosynthesis by CSRs is complex, but the current model begins with the gamma-butyrolactone SVB1 modulating the activity of the γ-butyrolactone receptor JadR3, which activates transcription of the gene encoding the OmpR-type atypical response regulator jadR1 and represses jadR2 .…”
Section: Coordinate Regulation Of Bgcsmentioning
confidence: 99%
“…Studies of compensation have focused on single resistances (e.g., [ 17 , 20 , 23 , 26 ]). However, resistance mutations often interact such that the fitness cost of multiple resistance tends to differ from that expected given the effects of each resistance [ 24 , 27 ]. These epistatic interactions might originate from the fact that resistance mutations affect interconnected functions in the cell [ 2 ].…”
Section: Introductionmentioning
confidence: 99%
“…Indeed, the reported yield for sugar modified 58a suggests production of 3 µgmL -1 in comparison to 22 µgmL -1 for its L-digitoxylated counterpart, Jd DS (36). 44,47 Likewise, for D-olivosylated jadomycin (58a) the reported yield suggested 0.3 µgmL -1 , in contrast to standard Jd B (2) reported to reach up to 69 µgmL -1 . 5,44,45 In all cases, these complemented systems were reliant on the relaxed specificity of native JadS to catalyze glycosyltransfer and deliver the sugar-modified jadomycin analogues.…”
Section: Sugar Modification Of Jadomycinsmentioning
confidence: 87%