Streptomyces xinghaiensis sp. nov., isolated from marine sediment

Abstract

A novel actinomycete, strain S187 T , was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0-9 % NaCl and at pH 6.0-9.0 and 10-45 6C. The cell wall of strain S187T contained the isomer LL-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H 6 ) (40.8 %), MK-9(H 8 ) (38.2 %) and MK-9(H 2 ) (8.8 %). The major fatty acids were iso-C 16 : 0 (29.6 %), anteiso-C 15 : 0 (14.0 %) and anteiso-C 17 : 0 (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The G+C content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of Streptomyces flavofuscus NRRL B-8036 T (5DSM 41426 T ) and Streptomyces albiaxialis DSM 41799 T , respectively, showing that the novel strain should be assigned to the genus Streptomyces. DNA-DNA hybridizations with the two above-mentioned Streptomyces species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187 T represents a novel species of the genus Streptomyces, for which the name Streptomyces xinghaiensis sp. nov. is proposed; the type strain is S187 T (5NRRL , 2006). Dalian is a coastal city located in the southernmost part of the Liaodong Peninsula, China, which has a very long coastline of 1906 km. However, the possible abundance of marine actinobacteria remains largely unexplored, except for some reports on sponge-derived marine actinobacteria (Zhang et al., 2006). During our screening of marine actinobacteria from sediment samples taken from the sea in the Dalian area, strain S187 T was recovered from a sediment sample collected from Xinghai Bay. In this study, strain S187T was characterized using a polyphasic taxonomic approach; it isThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain S187T is EF577247.A scanning electron micrograph of strain S187 T , a phylogenetic tree based on 16S rRNA gene sequences of S187T and related strains constructed using the maximum-parsimony method and antimicrobial activities of S187 T and closely related strains are available as supplementary material with the online version of this paper. proposed that this strain represents a novel species of the genus Streptomyces. International Journal of Systematic and Evolutionary MicrobiologyStrain S187 T was isolated from a marine sediment sample from Xinghai Bay, Dalian, China, after 2 weeks incubation at 28 u C on Bennett's agar (Margalith & Beretta, 1960). Morphological, physiological and biochemical characterizations of strain S187T were carried out following the standard protocol of the International Streptomyces Project (ISP; Shirling & Gottlieb, 1966, 1968a, b, 1969. Colour determination was assessed by comparison wit...

Streptomyces chumphonensis sp. nov., isolated from marine sediments

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