Streptomycin Induced Teratogenesis in Developing and Regenerating Amphibians

Abstract

This study shows that streptomycin has both the non-specific effect, short of toxicity, of disrupting developmental sequences in larval anurans and the phase specific effect of altering tail fin regeneration. Streptomycin seems to inhibit regeneration of mesenchymal elements while leaving ectodermal components able to proliferate though in quantitatively different amounts. It appears, therefore, that the regressive, histolytic stages of regeneration are not significantly affected by streptomycin, but the phases of cytoredifferentiation of muscle and connective tissue amounts and the regulation of somite pattern are progressively curtailed with exposure to the drug.

The Effect of Some Antibiotics on Protein Synthesis in Human Tonsillar Cells, <i>in</i><i>vitro</i>

“…Still, it should be noted that the tetracyclines proved to be teratogenic in mice [11]. Streptomycin inhibits the development and regeneration of amphibia; even those ani mals have undergone metamorphosis that did not show any abnormal ity [9].…”

The Inhibition of Limb Regeneration in Adult <i>Diemictylus viridescens </i>Treated with Streptomycin

“…Much recent information has accrued through the local and systemic application of various antimetabolites [see review in Schmidt, 1968], Of interest are the stud ies of Bieber and H itchings[I959], Burnet and Liversage[I964], who moni tored the effects of chloramphenicol on regenerating Triturus, Wolsky and Van Doi [1965] and Carlson [1967] who described the inhibitory effects of actinomycin D on anuran and urodelan regeneration. Recently we demon strated [Procaccini and Doyle, 1970b;Procaccini el a!., 1969] that exter nally-applied streptomycin retards and inhibits regenerative capacity in lar val Rana pipiens and R. clamitans and produces a specific teratologic re sponse. While our previous studies stressed quantitative aspects of inhibition and the definition of the streptomycin induced response in anurans, the pres ent study will attempt to present a macroscopic and histologic analysis of in hibition of limb regeneration in the adult newt, D. viridescens, by streptomy cin.…”