Streptomycin-resistant Plants from Callus Culture of Haploid Tobacco

Maliga, Pál; Breznovits, A.; Márton, László

doi:10.1038/newbio244029a0

Cited by 423 publications

(216 citation statements)

References 8 publications

Supporting

Mentioning

213

Contrasting

Order By: Relevance

“…Petit Havana SR1 [19], were grown in soil under greenhouse conditions. Flower buds of selected length were collected to obtain pollen of different developmental stages.…”

Section: Plant Materialsmentioning

confidence: 99%

Isolation and characterization of a microspore-specific gene from tobacco

et al. 1996

View full text Add to dashboard Cite

The characterization of a gene with a unique microspore-specific expression pattern is reported. Isolated microspores from tobacco were used to synthesize a cDNA library. Clones that did not hybridize to leaf cDNA were further characterized by northern analysis. One clone proved to be a microspore-specific cDNA, representing a transcript of 650 nt. The corresponding gene, NTM19 (Nicotiana tabacum microspore-specific), was isolated and its sequence analysed. The gene encodes a protein of 10.8 kDa with a pI of 6.92 and a putative signal sequence at the N-terminus. A localization study revealed a unique spatial and temporal distribution. The transcript was only detected in the unicellular microspore. No hybridization signals were observed in other pollen developmental stages, nor in the surrounding anther tissues or other vegetative tissues of the plant. Therefore it can be concluded that NTM 19 is a gene with a highly microspore-specific character according to both localization and stage of expression. Southern blot analysis demonstrated the presence of a small gene family. The occurrence of TNM 19 was investigated in a range of closely and distantly related species and was found to be present in other solanaceous species, including the ancestors of tobacco and in a monocot species.

show abstract

“…Petit Havana SR1 [19], were grown in soil under greenhouse conditions. Flower buds of selected length were collected to obtain pollen of different developmental stages.…”

Section: Plant Materialsmentioning

confidence: 99%

Isolation and characterization of a microspore-specific gene from tobacco

et al. 1996

View full text Add to dashboard Cite

show abstract

“…Protoplast transformation and selection of kanamycinresistant cell lines Nicotiana tabacum c.v. Petit Havana clone SRI (Maliga et al, 1973) protoplasts were chosen as the recipient system for gene transfer because (i) they are totipotent; (ii) their plating efficiency is high and reproducible; and (iii) they have good survival of treatments favouring DNA uptake. Freshly isolated mesophyll protoplasts were treated with the hybrid plasmids pABDI and pABDII using a DNA uptake procedure slightly modified from that developed by Krens et al (1982).…”

Section: Introductionmentioning

confidence: 99%

Direct Gene Transfer to Plants

Paszkowski¹,

Rd²,

Saul³

et al. 2007

Ciba Foundation Symposium 137 ‐ Applications of Plant Cell and Tissue Culture

View full text Add to dashboard Cite

Evidence for direct, gene-mediated stable genetic transformation of plant cells of Nicotiana tabacum is presented. A selectable hybrid gene comprising the protein coding region of the Tn5 aminoglycoside phosphotransferase type II gene under control of cauliflower mosaic virus gene VI expression signals was introduced into plant protoplasts as part of an Escherichia coli plasmid. The gene was stably integrated into plant genomic DNA and constitutively expressed in selected, drugresistant, protoplast-derived cell clones. The mode of integration of the foreign gene into the plant genome resembled that observed for DNA transfection of mammalian cells. Plants regenerated from transformed cell lines were phenotypically normal and fertile, and they maintained and expressed the foreign gene throughout the development of vegetative and generative organs. Microspores, grown in anther culture, developed into resistant and sensitive haploid plantlets. Genetic crossing analysis of one of the transformed plants revealed the presence of one dominant trait for kanamycin resistance segregating in a Mendelian fashion in the F1 generation.

show abstract

“…Among mutant lines of higher plants resistant to the antibiotic streptomycin, Nicotiana tabacum SRl [1,2] has been investigated most intensively.…”

Section: Introductionmentioning

confidence: 99%