Streptozotocin-induced chromosomal aberrations, SCEs and mutations in CHO-9 parental cells and in EM-C11 mutant cell line

Capucci, M.S.; Hoffmann, Maria E.; Zdzienicka, Małgorzata Z.; Natarajan, A.T.

doi:10.1016/0027-5107(94)00172-2

Cited by 11 publications

(1 citation statement)

References 24 publications

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“…Because STZ is usually used to experimentally induce diabetes mellitus in laboratory animals and since it has been considered as a potential compound for the clinical treatment of malignant diseases, there is an intensive search to establish the exact mechanisms underlying cytotoxicity by STZ. It has been shown that STZ is a potent alkylating agent which directly methylates DNA [7] giving rise to chromosome and DNA damage [8]. At the chromosome level, STZ induces chromosomal aberrations (CAs) and sisterchromatid exchanges (SCEs) in mammalian and insect cells [9].…”

Section: Introductionmentioning

confidence: 99%

Potential Protective Effect of Costus speciosus or its Nanoparticles on Streptozotocin-induced Genotoxicity and Histopathological Alterations in Rats

TMT¹

2015

J Nutr Food Sci

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Antigenotoxic properties of many medicinal plants have been widely recognized and some of them have been commercially exploited. Plant derived antioxidants play a very important role in alleviating problems related to oxidative stress. Therefore, the present study was aimed at assessing the protective effect of the medicinal plant Costus speciosus against genotoxicity and histopathological alterations of streptozotocin (STZ)-induced diabetes mellitus in male rats. Animals were divided into 8 groups (10 animals/group) as follows: 1) Streptozotocin (ip single dose of 50 mg/kg bw STZ , 3 d after the blood sugar was 200-250 mg/dl); 2) STZ + insulin (1 U for 14 days starting from d3 after STZ injection); 3) STZ + 50 mg/kg bw costus (oral administration); 4) STZ + 100 mg/kg bw costus; 5) STZ + 150 mg/kg bw costus; 6) STZ + 50 mg/kg bw nanocostus (oral administration); 7) STZ + 100 mg/kg bw nan ocostus; 8) STZ + 150 mg/kg bw nanocostus. Beside the control group (Citrate buffer). The results of the present study revealed that the treatment with STZ induced high frequency of micronuclei, DNA damage, chromosomal aberrations and histopathological changes. However, costus or nanocostus treatment reduced all these parameters especially with 50 mg/kg between nanocostus. This study proved that treatment with C. speciosus has a strong inhibitory effect against the genotoxicity and histopathologic alterations induced by STZ. In conclusion, these results strongly suggest that the extract of Costus speciosus might be anti-genotoxic, and anti-histopathologic agent.

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Section: Introductionmentioning

confidence: 99%

Potential Protective Effect of Costus speciosus or its Nanoparticles on Streptozotocin-induced Genotoxicity and Histopathological Alterations in Rats

TMT¹

2015

J Nutr Food Sci

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Interstitial telomeric sequences are not preferentially involved in the chromosome damage induced by the methylating compound streptozotocin in chinese hamster cells

Quiroga

Paviolo

Bolzán

2012

Environ and Mol Mutagen

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The effect of the methylating compound streptozotocin (STZ) on interstitial telomeric sequences (ITSs) was investigated in Chinese hamster ovary (CHO) cells by using peptide nucleic acid-fluorescence in situ hybridization with a pantelomeric probe. Cells were exposed to increasing concentrations of STZ, and chromosomal aberrations were analyzed at the first mitosis after treatment. The frequency of chromosomal aberrations directly involving ITSs increased in STZ-treated cells by a factor of 2.6 (2 mM) and 3.6 (4 mM) when compared with the frequency of these aberrations in control cells (P < 0.05). However, no significant differences were found between control and exposed cells in the percentage of aberrations directly involving ITSs, demonstrating that these repeat regions were not preferentially involved in the chromosome damage induced by STZ. In addition, STZ did not alter telomerase activity, suggesting that this enzyme may not be involved in the induction of chromosomal aberrations by this compound.

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Streptozotocin‐induced toxicity in CHO‐9 and V79 cells

Capucci

Hoffmann

Groot

et al. 1995

Environ and Mol Mutagen

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The cytotoxicity of streptozotocin (STZ) was investigated in Chinese hamster fibroblast lines (CHO-9 and V79) in comparison to two other alkylating agents, methylnitrosourea (MNU) and ethylnitrosourea (ENU), using cell survival as the endpoint. It was found that V79 cells were far more resistant to methylation induced by STZ and MNU than CHO-9 cells (20 and four times, respectively) but equally sensitive to the ethylating agent ENU. The extent of STZ-induced DNA methylation was estimated by analyzing the extent of O6-metG and N7-metG adducts in the DNA of treated cells through high-performance liquid chromatography (HPLC) with electrochemical detection. The number of adducts as well the efficiencies of their removal from the DNA were similar in both cell lines. The response of these cells to the presence of DNA damage was evaluated by analysis of STZ effects on DNA replication and cell cycle progression. Measurement of [3H]-thymidine incorporation showed a similar pattern of response at the level of inhibition of DNA synthesis in both cell lines. However, analysis of metaphase cells 36 hr after STZ exposure showed an accumulation of cells in the second cycle in the CHO-9 line, indicating induction of a cell cycle arrest. Only a slight effect was observed on cell cycle progression in V79 cells, indicating that the methylation resistance of these cells could be related to their ability to progress through the cell cycle despite the presence of DNA lesions.

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Streptozotocin-induced chromosomal aberrations, SCEs and mutations in CHO-9 parental cells and in EM-C11 mutant cell line

Cited by 11 publications

References 24 publications

Potential Protective Effect of Costus speciosus or its Nanoparticles on Streptozotocin-induced Genotoxicity and Histopathological Alterations in Rats

Potential Protective Effect of Costus speciosus or its Nanoparticles on Streptozotocin-induced Genotoxicity and Histopathological Alterations in Rats

Interstitial telomeric sequences are not preferentially involved in the chromosome damage induced by the methylating compound streptozotocin in chinese hamster cells

Streptozotocin‐induced toxicity in CHO‐9 and V79 cells

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