Stress alters food intake and glucosensing response in hypothalamus, hindbrain, liver, and Brockmann bodies of rainbow trout

Conde-Sieira, Marta; Aguilar, Ariel José; López‐Patiño, Marcos A.; Míguez, Jesús M.; Soengas, José L.

doi:10.1016/j.physbeh.2010.07.016

Cited by 55 publications

(55 citation statements)

References 29 publications

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“…As the amount of glucose was intended to be related to the glycemic conditions normally experienced by this species, we can conclude that the regulatory effect of CRF was apparently more important under hypo-and normo-glycemic conditions than under hyperglycemic conditions. Taking into account that, in a previous study, we had demonstrated that plasma cortisol levels in the same species were also dependent on glycemic conditions (Conde-Sieira et al, 2010a), these results allow us to suggest that the functioning of the HPI axis is modulated by glucose not only at the final step of the HPI axis (cortisol) but also at the very beginning within the brain (CRF), basically under hypo-or normo-glycemic conditions. The precise mechanism through which CRF is involved in the readjustment of glucosensing capacity remains to be elucidated.…”

Section: Discussion the Effects Of Crf On Glucosensing-related Paramesupporting

confidence: 53%

“…Those glucosensing systems in the hypothalamus and hindbrain are activated when glucose levels increase at the same time that food intake decreases; conversely, when glucose levels decrease, the glucosensors are inactivated and food intake increases (Polakof et al, 2007a;Polakof et al, 2008a;Polakof et al, 2008b). Moreover, those glucosensing mechanisms in hypothalamus, hindbrain and Brockmann bodies are readjusted under stress conditions, such as those associated with high stocking density, resulting in an inability of the system to respond to changes in levels of circulating glucose (Conde-Sieira et al, 2010a). Furthermore, the same stress conditions elicit changes in the mRNA abundance of several peptides related to the control of food intake, including CRF (Conde-Sieira et al, 2010b), that could be related to the well-known anorectic effect of stress in fish (Wendelaar Bonga, 1997;Bernier, 2006).…”

Section: Introductionmentioning

confidence: 99%

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CRF treatment induces a readjustment in glucosensing capacity in the hypothalamus and hindbrain of rainbow trout

Conde-Sieira

Librán-Pérez

López‐Patiño

et al. 2011

Journal of Experimental Biology

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SUMMARYStress conditions induced in rainbow trout a readjustment in the glucosensing response of the hypothalamus and hindbrain such that those sensors did not respond properly to changes in glucose levels, as demonstrated in previous studies. To evaluate the hypothesis that corticotropin-releasing factor (CRF) could be involved in that response, we have incubated the hypothalamus and hindbrain of rainbow trout at different glucose concentrations in the presence of different concentrations of CRF. Under those conditions, we evaluated whether parameters related to glucosensing [the levels of glucose, glycogen and glucose 6-phosphate, the activities of glucokinase (GK), glycogen synthase (GSase) and pyruvate kinase (PK), and mRNA abundance of transcripts for GK, Glut2, Kir.6-like and sulfonylurea receptor (SUR)-like] are modified in the presence of CRF in a way comparable to that observed under stress conditions. We obtained evidence allowing us to suggest that CRF could be involved in the interaction between stress and glucosensing as CRF treatment of the hypothalamus and hindbrain in vitro induced a readjustment in glucosensing parameters similar to that previously observed under stress conditions in vivo. We had also previously demonstrated that stress elicits alterations in food intake in parallel with the readjustment of glucosensing systems. Here, we provide evidence that the mRNA abundance of several of the neuropeptides involved in the regulation of food intake, such as neuropeptide Y (NPY) or cocaine-and amphetamine-regulated transcript (CART), is affected by CRF treatment, in such a way that their expression does not respond to changes in glucose levels in the same way as controls, allowing us to suggest that the food intake response that is integrated by changes in those peptides and known to be reduced by stress could be also mediated by CRF action in glucosensing areas.

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Section: Discussion the Effects Of Crf On Glucosensing-related Paramesupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

CRF treatment induces a readjustment in glucosensing capacity in the hypothalamus and hindbrain of rainbow trout

Conde-Sieira

Librán-Pérez

López‐Patiño

et al. 2011

Journal of Experimental Biology

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“…These data demonstrate that hyperglycemia can reliably be produced in the Giant Danio Devario aequipinnatus embryos, as has previously shown in Danio rerio species by [32,40]. The induced hypergylcemia in adult rainbow trout through manipulation of food source and stocking conditions [41]. Their study showed changes in cortisol levels in conjunction with glucose levels suggesting that hyperglycemia-induced stress results in changes in cortisol levels.…”

supporting

confidence: 73%

The Effects Induced Hyperglycemia on Adrenal Cortex Function in the Giant Danio Devario Aequipinnatus Embryos

Perumal¹

2017

JDMDC

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Purpose: The purpose of this study was to induce hyperglycemic experiment in Giant Danio embryos in relation to effects of hyperglycemia on adrenal cortex function and steroidogenesis. Methods:The wild specimens of Devario aequipinnatus fishes collected from the Cauvery river at Stanley Reservoir were induced for breeding without the use of any hormones and the matured embryos were obtained. The embryos of Giant Danio were induced for hyperglycemic stress, by immersing them in 0.0%, 0.3%, 0.6%, 0.9%, 1.2%, 1.5%, and 1.8% glucose solution, at 24 h, 48 h and 72 h. Result:The Giant Danio exposed to glucose solution appear to experience acute stress as indicated by the significant elevation in serum glucose and serum cortisol. The ranges of average cortisol level for all embryo samples exposed for 24 h, 48 h and 72 h in stock aquaria were: 1.2-6.4 µg/dL, 0.8 to 7.6 µg/dL and 0.8-9.6µg/dL respectively. Conclusion:The results showed positive correlation between the %glucose solution and the cortisol level, as well as the incubation time and the cortisol level as reported previously in other model systems. Keywords: The Effects Induced Hyperglycemia on Adrenal Cortex Function in the Giant Danio Devario Aequipinnatus Embryos 2/6Copyright: ©2017 Raja et al.gender, blood pressure, lifestyle, stress, perception of stress, and psychiatric illnesses are all known to affect cortisol secretion and metabolism as well as the increased risk of developing DM [28,29]. Using an animal model of DM would allow for control or elimination of many if not all of these confounding factors. Teleost fish, including Giant Danio (Devario aequipinnatus), have previously been shown to be glucose intolerant [27]. The experimental studies on hyperglycemia in relation to adrenal cortex are still needed, using fish as experimental model. Therefore, the present study was on the induced hyperglycemic experiment in Giant Danio embryos in relation to effects of hyperglycemia on adrenal cortex function and steroidogenesis. Materials and Methods Collection, domestication and maintenance of fishesThe wild specimens of Devario aequipinnatus were collected the Cauvery river at Stanley Reservoir, (N 11° 54'18.3" E 77° 53' 15.8") Salem District, Tamil Nadu, India, during September to March 2016, using drag net (Figure 1). The live fish samples were transported to the Laboratory in aerated bags and were maintained in a 700 L FRB tank with artificial, continuous aeration at a room temperature of 27-30 °C. The collected fishes, were stocked in rearing tanks of size 1.5 m x 0.5m x 1.5 m and mature males and females were kept separately with the density of 6-7 in each tank. The rearing tanks were provided with dark substrate (gravel and pebbles) and mild circulating water current with the help of electric motors for their adaptation. After proper acclimatization and maintenance, the domesticated brood stock was selected for the breeding experiments. The glass tank (1.5 m x 0.5 m x 1.5 m) with dark substrate and natural day lighting provided with spawning m...

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“…Furthermore, the same stress conditions elicit reduced food intake and changes in the mRNA abundance of several peptides related to the control of food intake, including corticotropin-releasing factor (CRF) (Conde-Sieira et al, 2010b), that could be related to the well-known anorectic effect of stress in fish (Wendelaar Bonga, 1997;Bernier, 2006). Accordingly, we have recently demonstrated that the responses to changes in glucose levels of parameters related to glucosensing in the hypothalamus and hindbrain of rainbow trout are modified in the presence of CRF (Conde-Sieira et al, 2011), in a way comparable to that observed under stress conditions (Conde-Sieira et al, 2010a).…”

Section: Introductionmentioning

confidence: 78%

“…We also demonstrated that these glucosensing systems are deregulated under stress conditions, like those associated with high stocking density (HSD), resulting in their inability to respond to changes in circulating glucose levels (Conde-Sieira et al, 2010a). Furthermore, the same stress conditions elicit reduced food intake and changes in the mRNA abundance of several peptides related to the control of food intake, including corticotropin-releasing factor (CRF) (Conde-Sieira et al, 2010b), that could be related to the well-known anorectic effect of stress in fish (Wendelaar Bonga, 1997;Bernier, 2006).…”

Section: Introductionmentioning

confidence: 93%

Glucosensing capacity in liver of rainbow trout displays day-night variations possibly related to melatonin action

Conde-Sieira

López‐Patiño

Míguez

et al. 2012

Journal of Experimental Biology

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SUMMARYTo assess whether the glucosensing capacity in peripheral (liver and Brockmann bodies) and central (hypothalamus and hindbrain) locations of rainbow trout displays day-night variations in its response to changes in circulating glucose levels, we evaluated the response of parameters related to glucosensing [glucose, glycogen and glucose 6-phosphate levels, activities of glucokinase (GK), glycogen synthetase (GSase) and pyruvate kinase (PK), and mRNA abundance of GK, glucose transporter 2 (GLUT2), and K ATP channel subunits Kir6.x-like and sulfonylurea receptor (SUR)-like] in fish subjected to hyperglycemic treatment under night or day conditions. No day-night significant variations were noticed in the glucosensing capacity of the hypothalamus, hindbrain and Brockmann bodies. In contrast, a clear differential response was noticed in the liver, where glucose levels, GK activity (and mRNA levels) and GSase activity displayed increased values during the day in hyperglycemic fish compared with controls, and lower (GK mRNA levels) or non-existent (glucose, GK and GSase activities, and Kir6.x-like mRNA levels) values during the night. A similar decrease in parameters related to glucosensing in the liver was observed when fish under day conditions were treated with melatonin, suggesting a modulatory role of melatonin in day-night changes of the glucosensing response in the same tissue.

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Stress alters food intake and glucosensing response in hypothalamus, hindbrain, liver, and Brockmann bodies of rainbow trout

Cited by 55 publications

References 29 publications

CRF treatment induces a readjustment in glucosensing capacity in the hypothalamus and hindbrain of rainbow trout

CRF treatment induces a readjustment in glucosensing capacity in the hypothalamus and hindbrain of rainbow trout

The Effects Induced Hyperglycemia on Adrenal Cortex Function in the Giant Danio Devario Aequipinnatus Embryos

Glucosensing capacity in liver of rainbow trout displays day-night variations possibly related to melatonin action

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