Water adsorption on coal plays a significant role in the process of CO 2 sequestration and enhanced coalbed methane recovery. Direct evidence of how coal adsorbs water and swells, affecting permeability, is still limited. Here, we studied the impact of waterflooding on fracture networks in coal by means of in situ synchrotron X-ray microtomography combined with permeability measurements. We demonstrated that swelling-induced fracture closure was responsible for an order of magnitude permeability reduction after waterflooding for over 4 days. Permeability loss was found to be time dependent, following a logistic function, and about 80% permeability reduction happened in the first 24 hr. There were probably three driven forces for water uptake, including hydrodynamic forces in fractures and macropores, capillary forces in micropores, and diffusion from micropores into deeper coal matrix. Swelling of coal matrix narrowed down and even closed the fractures and as a result weakened fracture connectivity. Residual fractures were mainly mineral-supported fractures, which have strong resistance to swelling-induced stresses.Plain Language Summary CO 2 storage in coal and simultaneously enhanced coalbed methane recovery (CO 2 -ECBM) is a global-warming mitigation technique with dual benefit. Porosity and permeability of coal are critical for CO 2 -ECBM, which determine the CO 2 storage capacity and methane productivity. Although it is widely reported that coalbed water adsorption on coal causes coal matrix swelling and reduces coal permeability, there is a lack of understanding the evolution of coal fracture networks and explaining the resulting permeability changes. We thus directly characterized and quantified the fracture network changes due to waterflooding using in situ synchrotron X-ray microtomography combined with permeability measurements. We identified the direct evidence-fracture closure resulting from adsorption-induced coal swelling-responsible for the permeability reduction after waterflooding. The existing fractures were found to narrow down readily and even close, causing an order of magnitude permeability reduction after waterflooding for over 4 days. Swelling process was found to be time dependent, and approximately 80% permeability reduction occurred in the first 24 hr. We also found that some residual fractures supported by minerals, which have strong resistance to the swelling-induced stresses, still remained after waterflooding.