Stress-induced curcin-L promoter in leaves of Jatropha curcas L. and characterization in transgenic tobacco

Qin, Xiaobo; Zheng, Xinglong; Shao, Chang‐Lun; Gao, Jihai; Jiang, Luding; Zhu, Xunlu; Yan, Fang; Tang, Lin; Xu, Ying; Chen, Fang

doi:10.1007/s00425-009-0956-9

Cited by 28 publications

(10 citation statements)

References 48 publications

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“…43,44 The activity of a curcin protein isoform against viral and fungal diseases has been proven by heterologous expression in tobacco; the expression of this curcin gene was induced by abiotic and biotic stresses in leaves. 45–47 So far, Jatropha genes encoding three isoforms of curcin have been reported and deposited in public DNA databases. In our Jatropha genome sequence, only three contigs were identified to encode amino acid sequences highly similar to those coding for curcin, confirming that the Jatropha genome contains three curcin genes.…”

Section: Resultsmentioning

confidence: 99%

Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L.

et al. 2010

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The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ∼95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at .

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Section: Resultsmentioning

confidence: 99%

Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L.

et al. 2010

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“…Some studies report that RIPs may be involved in the plant defense response [52–54]. Although RIPs have been classified into three groups, based on their structure and functions, type I RIPs are most important in J. curcas.…”

Section: Functional Genomics Analysis Of Economically Important Tramentioning

confidence: 99%

“…Curcin was isolated, cloned and sequenced [50, 54, 56]. Three contigs encoding amino acid sequences of curcin-like proteins were identified as highly similar to curcin [21].…”

Section: Functional Genomics Analysis Of Economically Important Tramentioning

confidence: 99%

“…Curcin-L was expressed and activated in the leaves of J. curcas by treatments with abscisic acid, salicylic acid, polyethylene glycol, extreme temperatures and ultraviolet light [54]. Curcin 2 was isolated from leaves that had been subjected to drought, temperature, and fungal infection stresses [57], suggesting a role in plant defense.…”

Section: Functional Genomics Analysis Of Economically Important Tramentioning

confidence: 99%

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Jatropha curcas, a biofuel crop: Functional genomics for understanding metabolic pathways and genetic improvement

Maghuly

Laimer

2013

Biotechnology Journal

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Jatropha curcas is currently attracting much attention as an oilseed crop for biofuel, as Jatropha can grow under climate and soil conditions that are unsuitable for food production. However, little is known about Jatropha, and there are a number of challenges to be overcome. In fact, Jatropha has not really been domesticated; most of the Jatropha accessions are toxic, which renders the seedcake unsuitable for use as animal feed. The seeds of Jatropha contain high levels of polyunsaturated fatty acids, which negatively impact the biofuel quality. Fruiting of Jatropha is fairly continuous, thus increasing costs of harvesting. Therefore, before starting any improvement program using conventional or molecular breeding techniques, understanding gene function and the genome scale of Jatropha are prerequisites. This review presents currently available and relevant information on the latest technologies (genomics, transcriptomics, proteomics and metabolomics) to decipher important metabolic pathways within Jatropha, such as oil and toxin synthesis. Further, it discusses future directions for biotechnological approaches in Jatropha breeding and improvement.

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“…A 654 bp fragment of a 5 0 flanking region preceding the curcin-L gene, designated CP2, was cloned from J. curcas and its expression was studied in transgenic tobacco via the expression of b-glucuronidase (GUS) gene. It was found that CP2 was leaf specific and could induce the expression of the reporter gene under stress conditions [37].…”

Section: Genes Associated With Abiotic Stress Tolerancementioning

confidence: 99%