Stress induced Salmonella Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages

Verbrugghe, Elin; Boyen, Filip; Parys, Alexander Van; Deun, Kim Van; Croubels, Siska; Thompson, Arthur R.; Shearer, Neil; Leyman, Bregje; Haesebrouck, Freddy; Pasmans, Frank

doi:10.1186/1297-9716-42-118

Cited by 63 publications

(61 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Researches indicated that CORT increased the proliferation of Salmonella in primary porcine alveolar macrophages [21]. Hosoda et al [22] revealed that estradiol and progesterone inhibited the growth of Helicobacter pylori in vitro culture [22].…”

Section: Discussionmentioning

confidence: 99%

The Changes of Fecal Steroid Hormones and Bacterial Composition in Different Gestation Stages of Meishan Sows

Xue¹,

Jiang²,

Liu³

et al. 2017

J Nutr Food Sci

View full text Add to dashboard Cite

Currently, the importance of gut microbiota to the health of their host has been well discussed, yet the knowledge about the host hormonal effect on the gut microbiota is limited. In this study, a combination of the high-throughput 16S rRNA gene-based pyrosequencing and the Mass Spectrometry (MS)-based metabolomics techniques were used to investigate the stool microbial composition and microbial metabolites at different gestation day in Meishan sows. The results showed that, the stool steroid hormones including estradiol (E 2 ), progesterone (P 4 ) and cortisol (CORT) were increased at gestation day 90 (P<0.05), when compared to gestation day 30. In coincide with the changes of steroid hormones, the relative abundance of Burkholderiales and Selenomonadales of the stool microbiota at d90 of pregnancy was significantly increased than that of at d30 of pregnancy at the order level (P<0.05). However, no significant difference of the richness estimators (ACE and Chao), and the diversity indices (Shannon and Simpson) were found between the d30 and d90 of gestation. Further, the metabolomics profile revealed 13 metabolites changed greatly from the gestation day 30 to 90, including amino acid metabolic pathway, lipid and carbohydrate metabolic pathway (P<0.001). The results suggest that the interaction of the steroid hormones and the gut bacteria may account for the metabolic changes throughout the length of pregnancy.

show abstract

Section: Discussionmentioning

confidence: 99%

The Changes of Fecal Steroid Hormones and Bacterial Composition in Different Gestation Stages of Meishan Sows

Xue¹,

Jiang²,

Liu³

et al. 2017

J Nutr Food Sci

View full text Add to dashboard Cite

show abstract

“…Stress is associated with a recrudescence of the infection (71,95). Handling, loading, mixing with unfamil iar pigs, high stocking densities, and changes in environ ment, including temperature, are some of the associated factors that promote stress.…”

Section: Salmonella At Slaughter In Swine Production: Influencing Facmentioning

confidence: 99%

Role of Slaughtering in Salmonella Spreading and Control in Pork Production

Argüello

Álvarez‐Ordóñez

Carvajal

et al. 2013

Journal of Food Protection

View full text Add to dashboard Cite

Salmonella is one of the major foodborne pathogens worldwide. Pork products are among the main sources of Salmonella infection in humans, and several countries have established Salmonella surveillance and control programs. The role of slaughtering in carcass contamination has been indicated by studies focused on the slaughterhouse environment. In this review, we examine and discuss the information available regarding the influence that farm status, pig transport, and lairage have on the carriage of Salmonella by pigs entering the slaughter line. The evolution of carcass contamination throughout the slaughtering process, the main sources of contamination in the dirty and clean zones of the slaughter line, and previously reported prevalence of Salmonella on carcasses and factors affecting this prevalence also are discussed. The importance of implementing interventions at the slaughter level is discussed briefly. Consistent with the information available, pigs from infected farms and newly acquired or recrudescent infections in pigs at the subsequent stages of transport and lairage are important sources of Salmonella at the slaughtering plant. The continuous introduction of Salmonella into the slaughterhouse and the potential for resident flora constitute a risk for carcass contamination. At the slaughterhouse, some dressing activities can reduce carcass contamination, but others are critical control points that jeopardize carcass hygiene. This information indicates the importance of considering slaughter and previous stages in the pork production chain for controlling Salmonella in swine production.

show abstract

“…Once a pig is infected with Salmonella, it will carry and shed Salmonella intermittently at low numbers throughout its life (Verbrugghe et al, 2011). Under stressful conditions, such as transportation and mixing of novel pigs (Callaway et al, 2006), pigs can resume fecal shedding and cross-contaminate pigs at lairage just before slaughter (Hurd et al, 2001), thus resulting in a higher prevalence of contaminated carcasses (Verbrugghe et al, 2011). The risk factors associated with Salmonella infection have been related to the size of the herds, the presence of birds within the barn, and the type of feeding (García-Feliz et al, 2009), but when implementing a comprehensive FIG.…”

Section: Discussionmentioning

confidence: 99%

Microencapsulated Sorbic Acid and Pure Botanicals Affect Salmonella Typhimurium Shedding in Pigs: A Close-Up Look from Weaning to Slaughter in Controlled and Field Conditions

Grilli

Foresti

Tugnoli

et al. 2015

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

The aim of this study was to assess the efficacy of a combination of sorbic acid, thymol, and carvacrol in reducing the prevalence and shedding level of Salmonella Typhimurium in pigs either in a controlled challenge environment or in a production setting. In the first study, 24 weaned piglets were separated in 4 isolation units (6 piglets/isolation unit). Each unit received either a basal diet (no treatment) or a microencapsulated mixture of sorbic acid, thymol, and carvacrol at 1, 2, or 5 g/kg of feed. After 21 d, pigs were orally challenged with 6 log10 colony-forming units of Salmonella Typhimurium. Blood samples and feces from rectal ampullae were collected every week. On d56 of the study, pigs were euthanized and necropsied to collect intestinal contents (jejunum through colon) and ileocecal lymph nodes. Samples were analyzed for Salmonella Typhimurium and serological analysis was also conducted. In the second study, an all-in-all-out multisite pig farm that was positive for monophasic Salmonella Typhimurium was followed throughout a production cycle from weaning to slaughter. Pigs received either a basal diet or the basal diet including 5 g/kg of the microencapsulated additive. Environmental, fecal, and blood samples were collected monthly, and cecal contents and ileocecal lymph nodes were collected at slaughter to isolate and enumerate Salmonella. The results indicate that the additive at 5 g/kg tended to reduce Salmonella fecal prevalence in both a controlled challenge (p=0.07) and in production conditions (p=0.03). Nevertheless, the additive did not reduce the number of pigs seropositive for Salmonella, nor it reduced the Salmonella prevalence at slaughter. The data indicate that these additives are not effective alone but must be used in conjunction with appropriate containment measures at lairage in order to prevent reinfection in pigs and to reduce the number of pigs carrying Salmonella entering the food chain.

show abstract

Stress induced Salmonella Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages

Cited by 63 publications

References 46 publications

The Changes of Fecal Steroid Hormones and Bacterial Composition in Different Gestation Stages of Meishan Sows

The Changes of Fecal Steroid Hormones and Bacterial Composition in Different Gestation Stages of Meishan Sows

Role of Slaughtering in Salmonella Spreading and Control in Pork Production

Microencapsulated Sorbic Acid and Pure Botanicals Affect Salmonella Typhimurium Shedding in Pigs: A Close-Up Look from Weaning to Slaughter in Controlled and Field Conditions

Contact Info

Product

Resources

About