Stress-inducible OsP5CS2 gene is essential for salt and cold tolerance in rice

Hur, Junghe; Jung, Kyungsook; Lee, Choon-Hwan; An, Gynheung

doi:10.1016/j.plantsci.2004.04.009

Cited by 138 publications

(79 citation statements)

References 40 publications

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“…Many species of temperate origin may develop tolerance when exposed to temperature change. This process is known as thermal adaptation that is associated to biochemical and physiological responses caused mainly by alterations in lipidic fluidity of membranes (Hur et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Alterations in fatty acid composition due to cold exposure at the vegetative stage in rice

Cruz

Golombieski

Bazana

et al. 2010

Braz. J. Plant Physiol.

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Rice is a tropical plant, so cold temperature may be detrimental to its development, depending on the genotype and environmental conditions. Degree of lipid unsaturation has been related to cold tolerance due to its effect on membrane stability. So, the aim of this study was to characterize the fatty acid composition and its alterations due to cold temperature in rice genotypes of diversified origin. Forty-four rice genotypes at the V 4 stage were submitted to two temperature conditions: 10°C and 28°C for two days and after this they had their leaves collected for lipid extraction and quantification. Control plants were allowed to regrow until presenting four leaves fully expanded and then were subjected to 10°C for ten days for cold tolerance evaluation. Plant survival was measured seven days after recovery at 28°C and the genotypes were grouped in three cold tolerance classes: tolerant, intermediate and sensitive. These classes differed for total saturated and unsaturated fatty acids only under the cold temperature treatment. Further analysis of the more abundant fatty acids: linoleic, linolenic and palmitic, showed that the two last ones differed between tolerant and sensitive genotypes. Linolenic acid increased after cold exposure in cold tolerant genotypes while palmitic acid decreased, and an opposite behavior was found in the cold sensitive genotypes. These evidences indicate that these fatty acids are potential molecular markers useful for breeding programs as well as for future basic studies on cold tolerance in rice.

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Section: Introductionmentioning

confidence: 99%

Alterations in fatty acid composition due to cold exposure at the vegetative stage in rice

Cruz

Golombieski

Bazana

et al. 2010

Braz. J. Plant Physiol.

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“…It has been confirmed that D-1-pyrroline-5-carboxylate synthetase is encoded by two differentially regulated genes in different plant species (Turchetto-Zolet et al, 2009). Usually one of the P5CS isoforms is osmo-regulated and the other is associated with developmentally governed processes (Hur et al, 2004;Székely et al, 2008;Pérez-Arellano et al, 2010).…”

Section: D-1-pyrroline-5-carboxylate Synthetase (P5cs)mentioning

confidence: 97%

Extreme climatic events: impacts of drought and high temperature on physiological processes in agronomically important plants

Feller

Vaseva

2014

Front. Environ. Sci.

112

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Climate models predict more frequent and more severe extreme events (e.g., heat waves, extended drought periods, flooding) in many regions for the next decades. The impact of adverse environmental conditions on crop plants is ecologically and economically relevant. This review is focused on drought and heat effects on physiological status and productivity of agronomically important plants. Stomatal opening represents an important regulatory mechanism during drought and heat stress since it influences simultaneously water loss via transpiration and CO 2 diffusion into the leaf apoplast which further is utilized in photosynthesis. Along with the reversible short-term control of stomatal opening, stomata and leaf epidermis may produce waxy deposits and irreversibly down-regulate the stomatal conductance and non-stomatal transpiration. As a consequence photosynthesis will be negatively affected. Rubisco activase-a key enzyme in keeping the Calvin cycle functional-is heat-sensitive and may become a limiting factor at elevated temperature. The accumulated reactive oxygen species (ROS) during stress represent an additional challenge under unfavorable conditions. Drought and heat cause accumulation of free amino acids which are partially converted into compatible solutes such as proline. This is accompanied by lower rates of both nitrate reduction and de novo amino acid biosynthesis. Protective proteins (e.g., dehydrins, chaperones, antioxidant enzymes or the key enzyme for proline biosynthesis) play an important role in leaves and may be present at higher levels under water deprivation or high temperatures. On the whole plant level, effects on long-distance translocation of solutes via xylem and phloem and on leaf senescence (e.g., anticipated, accelerated or delayed senescence) are important. The factors mentioned above are relevant for the overall performance of crops under drought and heat and must be considered for genotype selection and breeding programs.

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“…GSA kemudian berubah secara spontan menjadi pyrroline-5-carboxylate (P5C) dan P5C kemudian direduksi menjadi prolin oleh enzim P5C reductase (P5CR) (Kishor et al, 2005). Gen penyandi P5CS telah diisolasi dan diteliti lebih lanjut dari beberapa komoditas pertanian, di antaranya Phaseolus vulgaris (Chen et al, 2009;Chen et al, 2010), Sorghum bicolor (Su et al, 2011), Oryza sativa (Hur et al, 2004;Su & Wu, 2004), dan Triticum aestivum (Sawabel & Hasan., 2002).…”

Section: Pendahuluanunclassified

“…Aktivitas enzim P5CS pada tanaman padi transgenik yang ditransformasi dengan gen P5CS meningkat 5,3 hingga 9,8 kali, sedangkan kandungan prolinnya meningkat 167% sampai 252%. Di samping pening-katan akumulasi prolin, tanaman transgenik yang dihasilkan juga menunjukkan pertumbuhan yang lebih baik pada kondisi cekaman salinitas dan cekaman air jika dibandingkan dengan tanaman kontrol yang tidak ditransformasi dengan gen tersebut (Hur et al, 2004). Hasil penelitian Molinari et al (2007) menunjukkan bahwa pada tanaman tebu, akumulasi prolin berkorelasi positif dengan meningkatnya level ekspresi gen P5CS.…”

Section: Pendahuluanunclassified

“…Data tersebut dapat digunakan untuk identifikasi lanjutan dari gen P5CS pada tanaman. Menurut Hur et al (2004) tanaman padi mempunyai dua gen yang menyandi P5CS yaitu OsP5CS1 dan OsP5CS2. Data terkini melaporkan bahwa kedua gen family P5CS tersebut (P5CS1 dan P5CS2) pada Arabidopsis thaliana menunjukkan peranan yang berbeda di dalam mengakumulasi prolin pada kondisi cekaman kekeringan (Szekely et al, 2008).…”

Section: Soffic_p5csunclassified

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Kloning parsial gen penyandi P5CS dari tebu (Saccharum officinarum L.) Cloning of P5CS-encoding gene fragment from sugarcane (Saccharum officinarum L.)

MINARSIH¹,

SUPRIYADI²,

PUTRA³

et al. 2016

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AbstrakCekaman abiotik seperti kekeringan merupakan salah satu faktor penting yang mempengaruhi pertumbuhan tanaman. Tanaman mempunyai strategi adaptasi dalam mengatasi cekaman tersebut dengan mengakumulasi senyawa osmoprotektan yang terakumulasi dalam konsentrasi tinggi. Prolin merupakan salah satu senyawa osmoprotektan yang dapat melindungi tanaman dari cekaman kekeringan maupun osmotik. Salah satu enzim yang berperan penting dalam biosintesis prolin adalah ∆ 1 -pyrroline-5-carboxylate synthetase (P5CS) yang disandi oleh gen P5CS. Penelitian ini bertujuan untuk mengklon fragmen gen P5CS dari S. officinarum varietas PSJT 941. Amplifikasi fragmen gen P5CS dilakukan dengan teknik Reverse TranscriptionPolymerase Chain Reaction (RT-PCR) menggunakan primer spesifik gen P5CS. Fragmen DNA hasil RT-PCR berukuran 984 bp, 975 bp, dan 1725 bp diklon ke dalam Escherichia coli XL 1-Blue menggunakan vektor plasmid pGEM-T Easy. Hasil analisis BLAST menunjukkan bahwa sekuen fragmen gen produk RT-PCR yang berasal dari S. officinarum PSJT 941 memiliki homologi yang sangat tinggi (99%) dengan gen P5CS pada S. officinarum yang ada dalam pusat data Genbank.

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Stress-inducible OsP5CS2 gene is essential for salt and cold tolerance in rice

Cited by 138 publications

References 40 publications

Alterations in fatty acid composition due to cold exposure at the vegetative stage in rice

Alterations in fatty acid composition due to cold exposure at the vegetative stage in rice

Extreme climatic events: impacts of drought and high temperature on physiological processes in agronomically important plants

Kloning parsial gen penyandi P5CS dari tebu (Saccharum officinarum L.) Cloning of P5CS-encoding gene fragment from sugarcane (Saccharum officinarum L.)

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